Gram-negative endolysins: overcoming the outer membrane obstacle

Sisson, Hazel M; Jackson, Simon A; Fagerlund, Robert D; Warring, Suzanne L; Fineran, Peter C

doi:10.1016/j.mib.2024.102433

Cited by 2 publications

(2 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various studies have reported that membrane permeabilizers, such as chelators, cationic agents, and essential oils, enhance the sensitivity of Gram-negative bacteria to the bactericidal effects of endolysins, serving as adjuvants to potentiate or induce their action [58]. Consequently, the differences in activity observed across different strains in our study and previous reports are primarily attributed to variances in susceptibility to permeabilization treatment rather than disparities in their cell wall composition, which remains relatively consistent among Gram-negative bacteria [58][59][60]. In this context, Zermeño-Cervantes [61] evaluated the efficacy of KZ144 and LysPA26 endolysins against V. parahaemolyticus in natural seawater.…”

Section: Discussionmentioning

confidence: 99%

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Vibrio species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1’s lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only Vibrio alginolyticus GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio splendidus. Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.

show abstract

Section: Discussionmentioning

confidence: 99%

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Because the outer membrane (OM) of Gram-negative bacteria prevents externally applied endolysins from accessing peptidoglycan, controlling the growth of these Gram-negative pathogenic bacteria is difficult ( Sisson et al, 2024b ).…”

Section: Overcoming Challenges Of Gram-negative Endolysins Through Ge...mentioning

confidence: 99%

Endolysins: a new antimicrobial agent against antimicrobial resistance. Strategies and opportunities in overcoming the challenges of endolysins against Gram-negative bacteria

Khan,

Rasheed,

Yang

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Antibiotic-resistant bacteria are rapidly emerging, and the increasing prevalence of multidrug-resistant (MDR) Acinetobacter baumannii poses a severe threat to humans and healthcare organizations, due to the lack of innovative antibacterial drugs. Endolysins, which are peptidoglycan hydrolases encoded by a bacteriophage, are a promising new family of antimicrobials. Endolysins have been demonstrated as an effective therapeutic agent against bacterial infections of A. baumannii and many other Gram-positive and Gram-negative bacteria. Endolysin research has progressed from basic in vitro characterization to sophisticated protein engineering methodologies, including advanced preclinical and clinical testing. Endolysin are therapeutic agent that shows antimicrobial properties against bacterial infections caused by drug-resistant Gram-negative bacteria, there are still barriers to their implementation in clinical settings, such as safety concerns with outer membrane permeabilizers (OMP) use, low efficiency against stationary phase bacteria, and stability issues. The application of protein engineering and formulation techniques to improve enzyme stability, as well as combination therapy with other types of antibacterial drugs to optimize their medicinal value, have been reviewed as well. In this review, we summarize the clinical development of endolysin and its challenges and approaches for bringing endolysin therapies to the clinic. This review also discusses the different applications of endolysins.

show abstract

Gram-negative endolysins: overcoming the outer membrane obstacle

Cited by 2 publications

References 62 publications

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Endolysins: a new antimicrobial agent against antimicrobial resistance. Strategies and opportunities in overcoming the challenges of endolysins against Gram-negative bacteria

Contact Info

Product

Resources

About