Graft failure after allogeneic hematopoietic stem cell transplantation

Özdemi̇r, Zehra; Bozdağ, Sinem Civriz

doi:10.1016/j.transci.2018.04.014

Cited by 96 publications

(65 citation statements)

References 47 publications

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“…Evaluation of this was performed in most of the selected studies, which showed that the studies that used allogeneic cell transplantation had greater graft efficiency in a shorter time period than xenogeneic transplantation in the experimental model. Another relevant aspect of the success of transplantation is the functionality of cells after the graft, because graft failure and poor graft function are important issues in the current comprehensions of the interaction between the immune and hematopoietic compartments in these conditions [76]. Therefore, besides the quantitative analysis of the graft, the graft functionality should be adequate in the evaluation of graft efficiency, but this aspect was poorly approached in most of the selected studies.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Oliveira

Nucci

Filgueiras

et al. 2020

Cells

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The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis.

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Section: Discussionmentioning

confidence: 99%

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Oliveira

Nucci

Filgueiras

et al. 2020

Cells

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“…The majority of patients received a cyclophosphamide or busulfan-based myeloablative regimen as recommended. 12,30 Even so, non-myeloablative and reduced-intensity conditioning regimens have been successfully used in small case series, particularly in very Median days to neutrophil engraftment (range) 20 21 19 (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) .59…”

Section: Discussionmentioning

confidence: 99%

“…Primary graft failure (GF) was defined as no sign of neutrophil engraftment after 42 days for umbilical cord and 28 days for the others graft source. 28…”

Section: Me Thodsmentioning

confidence: 99%

Hematopoietic cell transplantation for Diamond Blackfan anemia: A report from the Pediatric Group of the Brazilian Bone Marrow Transplantation Society

Darrigo

Loth

Kuwahara

et al. 2020

European J of Haematology

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Objectives The aim of this study was to analyze the outcomes of children with Diamond‐Blackfan anemia (DBA) treated in Brazil with hematopoietic cell transplantation (HCT). Methods We performed a retrospective analysis of 44 pediatrics patients transplanted between 1990 and 2018. The median age of patients was 5 years, and 57% were male. Twenty‐five received their first HCT from an HLA‐matched sibling donor (MSD), 12 from a HLA matched unrelated bone marrow donor (MUD 10/10, n = 12) and 7 other HLA mismatched donors (MMD). Results After a median follow‐up of 4 years, estimate 5‐year overall survival (OS) for the entire cohort was 70%, 80% for MSD group, 73% for MUD, and 29% for MMD. Thirty‐eight out of the 44 evaluable patients engrafted successfully. Primary and secondary graft failure was observed in five and three patients, respectively. Rates of grade II‐IV and III‐IV acute graft‐versus‐host disease (aGVHD) were 25% and 18%, respectively. Nine patients developed chronic GVHD (cGVHD). Conclusion Overall survival rates observed after HLA matched donors transplant for DBA were comparable to those reported from higher‐income countries and international registries.

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“…13,18 However there is a high rate of failure associated with unsuccessful initial engraftment, loss of donor cells following engraftment, or low stem cell dose. 56 Increased success has been correlated with an increased number of donor HSCs infused, and thus methods of expansion prior to transplant has been an active area of investigation. 19 Currently, there is no reliable method of expanding HSCs without leading to exhaustion following transplant.…”

Section: Introductionmentioning

confidence: 99%

Perivascular Secretome Influences Hematopoietic Stem Cell Maintenance in a Gelatin Hydrogel

Barnhouse

Petrikas

Crosby

et al. 2020

Preprint

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Adult hematopoietic stem cells (HSCs) produce the body's full complement of blood and immune cells. They reside in specialized microenvironments, or niches, within the bone marrow. The perivascular niche near blood vessels is believed to help maintain primitive HSCs in an undifferentiated state but demonstration of this effect is difficult. In vivo studies make it challenging to determine the direct effect of the endosteal and perivascular niches as they can be in close proximity, and two-dimensional in vitro cultures often lack an instructive extracellular matrix environment. We describe a tissue engineering approach to develop and characterize a three-dimensional perivascular tissue model to investigate the influence of the perivascular secretome on HSC behavior. We generate 3D endothelial networks in methacrylamide-functionalized gelatin hydrogels using human umbilical vein endothelial cells (HUVECs) and mesenchymal stromal cells (MSCs). We identify a subset of secreted factors important for HSC function, and examine the response of primary murine HSCs in hydrogels to the perivascular secretome. Within 4 days of culture, perivascular conditioned media promoted maintenance of a greater fraction of hematopoietic stem and progenitor cells. This work represents an important first-generation perivascular model to investigate the role of niche secreted factors on the maintenance of primary HSCs.

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Graft failure after allogeneic hematopoietic stem cell transplantation

Cited by 96 publications

References 47 publications

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Hematopoietic cell transplantation for Diamond Blackfan anemia: A report from the Pediatric Group of the Brazilian Bone Marrow Transplantation Society

Perivascular Secretome Influences Hematopoietic Stem Cell Maintenance in a Gelatin Hydrogel

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