2012
DOI: 10.1016/j.freeradbiomed.2012.02.043
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Abstract: Glutathione peroxidase 4 (Gpx4) is an antioxidant defense enzyme important in reducing hydroperoxides in membrane lipids and lipoproteins. Gpx4 is essential for survival of embryos and neonatal mice; however, whether Gpx4 is required for adult animals remains unclear. In this study, we generated a floxed Gpx4 mouse (Gpx4(f/f)), in which exons 2–4 of Gpx4 gene are flanked by loxP sites. We then cross-bred the Gpx4(f/f) mice with a tamoxifen (tam)-inducible Cre transgenic mouse (R26CreER mice) to obtain mice in … Show more

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Cited by 187 publications
(130 citation statements)
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“…Animals and Procedures-The generation of Gpx4(f/f) mice has been described previously (10). The Slick H mice and Camk2␣-creERT mice were obtained from The Jackson Laboratories (Bar Harbor, ME).…”
Section: Methodsmentioning
confidence: 99%
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“…Animals and Procedures-The generation of Gpx4(f/f) mice has been described previously (10). The Slick H mice and Camk2␣-creERT mice were obtained from The Jackson Laboratories (Bar Harbor, ME).…”
Section: Methodsmentioning
confidence: 99%
“…We previously generated Gpx4(f/f) mice, a mouse model with floxed Gpx4 alleles (10). To generate a conditional neuron-specific Gpx4 knockout mouse, we crossbred Gpx4(f/f) mice with single-neuron labeling with inducible Cre-mediated knockout, H line (Slick H) mice, which have a Slick transgene that expresses Cre recombinase fused to a mutated ligand-binding domain of the human estrogen receptor (creERT) and YFP/GFP in neurons (12,13), and obtained mice with two floxed Gpx4 alleles and the Slick transgene (Gpx4(f/f);Slick).…”
Section: Paralysis Muscle Atrophy and Death Of Gpx4niko Micementioning
confidence: 99%
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“…Gpx4 is the most abundant lipid hydroperoxide reductase and is shared among the mitochondria, nucleus and cytosol [144]. Deletion of Gpx4 is embryonic lethal [63] and recently, it was shown that cre-recombinase-dependent ablation of Gpx4 results in the death of adult mice in two weeks, underscoring the critical importance of this enzyme [146]. Gpx4 reduces lipid hydroperoxides and hence terminates species that can mitigate lipid peroxidation propagation reactions.…”
Section: Antioxidant Knockout Mice With Lethal Phenotypesmentioning
confidence: 99%
“…The genetic knockout of SOD2 or thioredoxin-2 (TRX2), which plays a vital role in PRX3 reactivation, is embryonically lethal (Hamilton et al, 2012). Loss of glutathione peroxidase-1 (GPX1) accelerates oxidative stress and damage and removing GPX4, which preferentially detoxifies lipid hydroperoxides, is also embryonically lethal (Ardanaz et al, 2010;Yoo et al, 2012). Exposure of mammals to O 2 and its reactive intermediates is unavoidable.…”
Section: Introductionmentioning
confidence: 99%