Gonadotropin release from the pars distalis of goldfish, Carassius auratus, transplanted beside the brain or into the brain ventricles: Additional evidence for gonadotropin-release-inhibitory factor

The effect of luteinizing hormone-releasing hormone analogue (LHRHa) in combination with different drugs with anti-dopamine and anti-serotonin properties on gonadotropin release and ovulation in the African catfish, Clarias gariepinus

“…The low frequency of ovulation resulting from LHRHa treatment alone has been fully understood following the results of lesion and pars distalis transplantation studies in goldfish Peter and Paulencu, 1980;Peter et al, , 1984. The surge of GTH that precedes oocyte maturation and ovulation is the result of a stimulation by GnRH in combination with a decreased inhibition by GnRIF.…”

Antisense for gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio)

“…Blood was extracted from the caudal vessel of the AS(+) transgenic fish without sperm (n = 65) and controls (n = 34) and stored at 4°C for 3-4 h. Serum was separated by centrifugation and frozen at − 70°C until tested. The GtH content in 50 μl plasma was measured by specific radioimmunoassays (RIA) (Peter et al, 1984;Wang et al, 1997) using a standard carp GtH-II protein and a rabbit-anti-carp-GtH-II antibody (RacGtH-II). Goat-anti-γ-globulin serum (GAR) (Arnel, USA) was used as the secondary antibody.…”

In vitro and in vivo effects of ghrelin on luteinizing hormone and growth hormone release in goldfish

“…GH and LH levels in the perifusate fractions, static culture samples, and serum samples were measured using previously validated RIAs for GH (28) and LH (37). All samples were assayed in duplicate in the same RIA.…”