Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

Rugaie, Osamah Al; Jabir, Majid S.; Kadhim, Rua J.; Karsh, Esraa H.; Sulaiman, Ghassan M.; Mohammed, Salman A. A.; Khan, Riaz A.; Mohammed, Hamdoon A.

doi:10.3390/microorganisms9010101

Cited by 26 publications

(13 citation statements)

References 65 publications

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“…This creates a great desire to synthesize nanoparticles with harnessed size and nanometer morphology in an eco-friendly mode [11]. The size and the shape of nanoparticles are permanently related to the unique characteristics, work, and application of nanomaterials [12,13]. Because of its eco-friendliness, low toxicity along with increased stability, small size, and shape distribution, emerging advances in nanoparticle generation using green syntheses are attracting much attention today [14,15].…”

Section: Introductionmentioning

confidence: 99%

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

Dhanraj

Rajeshkumar

2021

Journal of Nanomaterials

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Selenium is a trace element in the human body present in various enzymes with antioxidant activities and several functional proteins. This study is aimed at synthesizing selenium nanoparticles using Brassica oleracea (broccoli) and characterizing and assessing the antioxidant and antimicrobial effectiveness against cariogenic microorganisms. UV-visible spectrum displayed a peak at 370 nm which confirms the formation of SeNPs. TEM images of synthesized selenium nanoparticles showed polydisperse nanoparticles, spherical. The size of the particles ranged from 10 to 25 nm, and the average particle size obtained was 15.2 ± 1.9 nm . SEM images of nanoparticles were spherical and ranged in size from 10 to 28 nm. The SeNPs showed effective antimicrobial activity against cariogenic pathogens. The SeNPs synthesized with Brassica oleracea extract can be incorporated in toothpaste, gums, and mouthwashes that are cost-effective and biocompatible and used for the prevention of dental caries.

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Section: Introductionmentioning

confidence: 99%

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

Dhanraj

Rajeshkumar

2021

Journal of Nanomaterials

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“…Monocytes realize defense functions against pathogens via numerous mechanisms including phagocytosis and intracellular killing. Previous works have reported that graphene-based and other nanomaterials can both enhance [ 38 , 39 ] and suppress [ 40 , 41 ] the ability of phagocytic cells to engulf pathogens.…”

Section: Resultsmentioning

confidence: 99%

Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood

et al. 2021

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Multiple graphene-based therapeutics have recently been developed, however potential risks related to the interaction between nanomaterials and immune cells are still poorly understood. Therefore, studying the impact of graphene oxide on various populations of immune cells is of importance. In this work, we aimed to investigate the effects of PEGylated graphene oxide on monocytes isolated from human peripheral blood. Graphene oxide nanoparticles with lateral sizes of 100–200 nm and 1–5 μm were modified with linear and branched PEG (GO-PEG). Size, elemental composition, and structure of the resulting nanoparticles were characterized. We confirmed that PEG was successfully attached to the graphene oxide surface. The influence of GO-PEG on the production of reactive oxygen species (ROS), cytokines, phagocytosis, and viability of monocytes was studied. Uptake of GO-PEG by monocytes depends on PEG structure (linear or branched). Branched PEG decreased the number of GO-PEG nanoparticles per monocyte. The viability of monocytes was not altered by co-cultivation with GO-PEG. GO-PEG decreased the phagocytosis of Escherichia coli in a concentration-dependent manner. ROS formation by monocytes was determined by measuring luminol-, lucigenin-, and dichlorodihydrofluorescein-dependent luminescence. GO-PEG decreased luminescent signal probably due to inactivation of ROS, such as hydroxyl and superoxide radicals. Some types of GO-PEG stimulated secretion of IL-10 by monocytes, but this effect did not correlate with their size or PEG structure.

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“…The Student›s t-test was also used to test for significant differences in means. The p value <0.05 was considered to be significant (Al Rugaie et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Synthesis, docking study, and in vitro anticancer evaluation of new flufenamic acid derivatives

Al-Bayati¹,

Mahmood²,

Al-Mazaydeh³

et al. 2021

PHAR

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Novel compounds (6–10) were synthesized and confirmed by spectroscopic analysis, including AT-IR, 1HNMR and CHNS. Their cytotoxic effect was evaluated by MTT assay against two cancer cell lines and two normal cell types. Compound 7 exhibited anticancer activity against MCF-7 breast cancer cell line (GI50 = 63.9 µg/ml, 148 µM), without any effect against A549 lung cancer cells, or the normal cells. Compound 7 caused cytotoxicity in MCF-7 breast cancer cells by apoptotic cell death, as suggested by fragmented nuclei after DAPI staining and agarose gel electrophoresis. In addition, treating MCF-7 cells with compound 7 resulted in an increase in the level of caspase 9 mRNA level, and its activation. Moreover, compound 7-treated MCF-7 cells showed enhanced cytochrome c release from the mitochondria to the cytosol, signifying an induction of the intrinsic apoptotic pathway. Finally, compound 7 exhibited epidermal growth factor receptor (EGFR) kinase inhibitory activity at (EC50 = 0.13 µM), which was matched by molecular docking studies that showed compound 7 might be an important EGFR kinase inhibitor.

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Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

Cited by 26 publications

References 65 publications

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

Interaction of Graphene Oxide Modified with Linear and Branched PEG with Monocytes Isolated from Human Blood

Synthesis, docking study, and in vitro anticancer evaluation of new flufenamic acid derivatives

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