Gold-Nanoparticle-Probe–Based Assay for Rapid and Direct Detection of Mycobacterium tuberculosis DNA in Clinical Samples

Baptista, Pedro V.; Kozioł-Montewka, Maria; Paluch-Oleś, Jolanta; Dória, Gonçalo; Franco, Ricardo

doi:10.1373/clinchem.2005.065391

Cited by 190 publications

(129 citation statements)

References 11 publications

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“…For example, direct examination of clinical samples has successfully demonstrated M. tuberculosis DNA after an initial round of PCR, using a colorimetric method based on an M. tuberculosis probe linked to gold nanoparticles (Baptista et al, 2006).…”

Section: Other Methods Of Mtbc Adna Detection In Liquid Systemsmentioning

confidence: 99%

Molecular Biomarkers for Ancient Tuberculosis

Minnikin¹,

Lee²,

Wu³

et al. 2012

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

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Section: Other Methods Of Mtbc Adna Detection In Liquid Systemsmentioning

confidence: 99%

Molecular Biomarkers for Ancient Tuberculosis

Minnikin¹,

Lee²,

Wu³

et al. 2012

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

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“…It is a promising field as more and improved techniques are becoming available for clinical diagnostics with increased sensitivity at lower costs (Baptista et al, 2008;Baptista et al, 2005;Doria et al, 2007b;Baptista et al, 2006).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

“…This method has been successfully applied to detect eukaryotic gene expression without retro-transcription or PCR amplification steps (Conde et al, 2010b;Baptista et al, 2005); to distinguish fully complementary from mismatched sequences, with a single base mismatch i.e. to detect common mutations within the β-globin gene (Doria et al, 2007b); and in a fast and straightforward assay for Mycobacterium tuberculosis DNA detection in clinical samples (Costa et al, 2009;Baptista et al, 2006).…”

Section: Nanodiagnosticsmentioning

confidence: 99%

Multifunctional Gold Nanocarriers for Cancer Theranostics: From Bench to Bedside and Back Again?

Conde

Tian²,

Baptista

et al. 2014

Nano-Oncologicals

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After a quarter of century of rapid technological advances, research has revealed the complexity of cancer, a disease intimately related to the dynamic transformation of the genome. However, the full understanding of the molecular onset of this disease is still far from achieved and the search for mechanisms of treatment will follow closely.It is here that Nanotechnology enters the fray offering a wealth of tools to diagnose and treat cancer.It is indisputable that the use of gold nanocarriers has been gaining momentum as vectors for therapy and diagnostic strategies, combining the AuNPs' ease of functionalization with numerous biomolecules, high loading capacity and fast uptake by target cells. In fact, over the last decade nearly 12.000 research papers focusing on multifunctional gold nanocarriers have been published in peer-reviewed journals. Some of the described nanosystems will most likely revolutionize our understanding of biological mechanisms and push forward the clinical practice through their 2 integration in future diagnostics platforms. Nevertheless, very little gave fruitful results in order to improve a bench-to-bedside approach to translational research. On the basis of theoretical and experimental results obtained so far are we or not at the point: from bench to bedside and back again? As you will see, the answers to this question are complex, but one thing is clear: Translation into clinics is a tortuous and difficult path. Here, we provide a critical review about the available multifunctional gold nanocarriers for in vitro application and in vivo cancer targeting on nanodiagnostics and therapy.

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“…The first application of AuNPs for the molecular diagnostics of M. tuberculosis was introduced by Baptista et al (Baptista et al, 2006). The method consists in differential stabilization of gold nanoprobes in the presence of different DNA targets.…”

Section: Noble Metal Nanoparticlesmentioning

confidence: 99%

“…The gold nanoprobes were functionalized with thiol-modified oligonucleotides harboring a sequence derived from the M. tuberculosis RNA polymerase β-subunit gene sequence suitable for mycobacteria identification. The methodology was tested in clinical samples demonstrating high efficiency when combined with an initial round of PCR for target amplification (Baptista et al, 2006) -see Figure 1.…”

Section: Noble Metal Nanoparticlesmentioning

confidence: 99%