Gold Nanoparticle/Carbon Fiber Hybrid Structure from the Eco-Friendly and Energy-Efficient Process for Electrochemical Biosensing

Abstract

The carbon fiber/metal nanoparticle hybrid structure is a widely studied material combination for various fields such as energy storage, highperformance composites, and biomedical tools. The versatile carbon fiber/gold nanoparticle structure was prepared using an eco-friendly and energy-efficient fabrication process to address the sustainability issue. First, cellulose fiber was prepared by an eco-friendly lyocell process. The study obtained high mechanical strength of the lyocell fiber through high cellulose contents, strict pulp dissolution conditions, modified spinning, and thermal elongation processes. Then, the lyocell fiber was changed to carbon fiber with stabilization under 5% tension and carbonization, resulting in high mechanical strength and Young's modulus. Finally, gold nanoparticles were electrochemically deposited on the lyocell-based carbon fiber surface. The electrochemical properties of the carbon fiber/gold nanoparticle system were drastically improved due to the high surface area of the nanostructured gold particles. Enhanced electrochemical property and biocompatibility from non-toxic material selection enabled the carbon fiber/gold nanoparticles for dopamine detection, a representative electrochemical biosensor. The detection result demonstrated that the hybrid structure provided high sensitivity and selectivity for dopamine sensing. Overall, this fabrication strategy opens up numerous application opportunities to address eco-friendly and sustainable development requirements.

Robust and conductive cellulose hydrogel generated by liquid metal particles for biomedical application

“…A statistical study clari ed the recording performance stability of LMP5 within 50 measurement cycles. The averaged SNR from each cycle (after5,10,15,20,30,40,50) showed no signi cant difference (p > 0.05) compared to SNR from the rst measurement. Hence, the EMG measurement result concludes the LMP5 prepared in this study has good biocompatibility, functionality, and biostability, presenting the LMPs introduced cellulose hydrogel has good potential to use in biomedical applications.…”

“…Next, 20 µL of the supplied 2 mM ethidium homodimer (EthD-1) stock solution and 5 µL of the 4 mM calcein AM stock solution were added to 10 mL of 0.01M PBS (1xPBS), as directed in the protocol. Lastly, 2 mL of mixture solution was introduced to each well; all the methods and protocols were mentioned in the previous study (Lim et al 2022c). The mixture was incubated for 30 min in the live/dead dye solution and monitored by uorescence microscopy (EVOS FL Cell Imaging System, Thermo Fischer Scienti c, USA).…”

Conductive and Robust Cellulose Hydrogel Generated by Liquid Metal for Biomedical Applications

“…Next, 20 μL of the supplied 2 mM ethidium homodimer (EthD-1) stock solution and 5 μL of the 4 mM calcein AM stock solution were added to 10 mL of 0.01 M PBS (1× PBS), as directed in the protocol. Last, 2 mL of mixture solution was introduced to each well; all the methods and protocols were mentioned in a previous study . The mixture was incubated for 30 min in a live/dead dye solution and monitored by fluorescence microscopy (EVOS FL Cell Imaging System, Thermo Fischer Scientific, USA).…”

“…Last, 2 mL of mixture solution was introduced to each well; all the methods and protocols were mentioned in a previous study. 42 The mixture was incubated for 30 min in a live/dead dye solution and monitored by fluorescence microscopy (EVOS FL Cell Imaging System, Thermo Fischer Scientific, USA).…”

Liquid Metal-Based Electronic Textiles Coated with Au Nanoparticles as Stretchable Electrode Materials for Healthcare Monitoring

“…Au NPs are known to offer superior electrochemical performance and biocompatibility due to their biostability. 39,53 To confirm the biocompatibility of the AuLM textile under different conditions (stretched, unstretched, and multiple-time stretched), we conducted a cytotoxicity test using 3T3 cells. The cells were cultured and incubated with 5 × 5 mm 2 that most cells were dead with LM fabrics after 1 day and that green-stained cells (live cells) were rarely observed after 4 days (Figure 5a).…”