Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection

Guirgis, Bassem S.; Cunha, Claudia Sa E; Gomes, Inês; Cavadas, Miguel; Silva, Isabel; Dória, Gonçalo; Blatch, Gregory L.; Baptista, Pedro V.; Pereira, Eulália; Azzazy, Hassan M.E.; Mota, Maria M.; Prudêncio, Miguel; Franco, Ricardo

doi:10.1007/s00216-011-5489-y

Cited by 74 publications

(46 citation statements)

References 28 publications

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“…Very recently, Guirgis et al [62] described a homogeneous assay based on the fluorescence quenching of cyanine 3B (Cy3B)-labeled recombinant P. falciparum heat shock protein 70 (PfHsp70) upon binding to AuNP functionalized with an anti-Hsp70 monoclonal antibody. Upon competition with the free antigen, the Cy3B-labeled recombinant PfHsp70 is released to solution resulting in an increase of fluorescence intensity.…”

Section: Detection Methods Using Nanogoldmentioning

confidence: 99%

The role of nanogold in human tropical diseases: research, detection and therapy

Almeida

Carabineiro

2013

Gold Bull

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Section: Detection Methods Using Nanogoldmentioning

confidence: 99%

The role of nanogold in human tropical diseases: research, detection and therapy

Almeida

Carabineiro

2013

Gold Bull

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“…Au NP-fluorophore complexes show promise for in vitro diagnostic applications as "noses," with the ability to discern between various bacteria species and strains, proteins in complex solutions, and between cancerous and healthy cell lines [470]. These complexes have also been used for the detection of malaria antigens [471], DNA analysis [472,473], and for in vivo probes for reactive oxygen species, hyaluronidase, and protease detection systems [458]. Aptamers modified with fluorophores and subsequently combined with Au NPs have been used for the multiplex detection of adenosine, K þ ions and cocaine [474], and nanorulers for measuring binding-site distances on live cell surfaces [475].…”

Section: 53mentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

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“…The label will be conjugated to antibody and embedded into conjugate pad. There are various methods available for cross-linking antibody to AuNP [5,6]. The electrostatic adsorption of the antibodies to the AuNPs surfaces is the most commonly used strategies due to its simplicity in practice.…”

Section: Introductionmentioning

confidence: 99%

Covalent Conjugation of Antibody and Gold Nanoparticle for Development of Lateral Flow Immunoassay Test Strip

Phong¹

2018

JST

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Nanotechnology is one of the fastest growing technologies in this era. Gold nanoparticle (AuNP) based immunoassays have been performed on the basis of antigen-antibody interaction using AuNP antibody conjugates. Lateral flow immunoassays(LFA) which are also based on AuNP antibody conjugates are useful innovation in nanotechnology and widely applied in medicine and research fields. However, there are some limitations of the present LFA kits such as sensitivity and stability. In the study, we showed the result of covalent conjugation of antirotavirus antibody and AuNP for generating a lateral flow immunoassay strip to detect rotavirus in fecal samples. The suitable conditions for coating polyethylene glycol (PEG) on the surface of AuNP were 10.0 M PEG for 3 hours at room temperature (25 o C). Optimized conditions for covalent conjugation of antibody and AuNP were pH 4.0, 0.1 g antibody/conjugate, 0.01 mM reactant EDC/NHS [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/(N-hydroxy sulfosuccinimide]. The coupling reaction was carried out at room temperature for 90 min. The conjugate pad, antibody immobilized nitrocellulose membrane strip were created with investigated conditions for generating an LFA test strip. The limit of detection of LFA test strip was determined by 1.6 × 10 5 virus particles/ml, three times lower than that of Rotaclone kit (UK). The generated strip could be used to detect rotavirus in fecal sample of patient.

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Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection

Cited by 74 publications

References 28 publications

The role of nanogold in human tropical diseases: research, detection and therapy

The role of nanogold in human tropical diseases: research, detection and therapy

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Covalent Conjugation of Antibody and Gold Nanoparticle for Development of Lateral Flow Immunoassay Test Strip

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