Goat’s αS1-casein polymorphism affects gene expression profile of lactating mammary gland

Ollier, Séverine; Chauvet, Stéphanie; Martin, Patrice; Chilliard, Yves; Leroux, Christine

doi:10.1017/s1751731108001584

Cited by 28 publications

(12 citation statements)

References 36 publications

(41 reference statements)

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“…FAS of animal tissues is a homodimer, and each monomer carries seven catalytic domains and an acyl carrier protein in the following order: ketoacyl synthase, acetyl/malonyl transacylase, dehydratase, enoyl reductase, ketoacyl reductase, acyl carrier protein, and thioesterase . In the goat species, numerous studies have reported that FAS is regulated at the transcriptional level . Our study reveals that caprine FAS is also very likely regulated at the posttranslational level, according to the presence of up to 33 phosphosites through the entire protein backbone.…”

Section: Discussionmentioning

confidence: 74%

Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell

Henry¹,

Saadaoui

Bouvier³

et al. 2015

Proteomics

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Mechanisms of milk lipid secretion are highly controversial. Analyzing the fine protein composition of the "milk fat globule membrane" (MFGM), the triple-layered membrane surrounding milk lipid droplets (LDs) can provide mechanistic clues to better understand LD biosynthesis and secretion pathways in mammary epithelial cells (MECs). We therefore combined a high-sensitive Q-Exactive LC-MS/MS analysis of MFGM-derived peptides to the use of an in-house database intended to improve protein identification in the goat species. Using this approach, we performed the identification of 442 functional groups of proteins in the MFGM from goat milk. To get a more dynamic view of intracellular mechanisms driving LD dynamics in the MECs, we decided to investigate for the first time whether MFGM proteins were phosphorylated. MFGM proteins were sequentially digested by lysine-C and trypsin proteases and the resulting peptides were fractionated by a strong cation exchange chromatography. Titanium beads were used to enrich phosphopeptides from strong cation exchange chromatography eluted fractions. This approach lets us pinpoint 271 sites of phosphorylation on 124 unique goat MFGM proteins. Enriched GO terms associated with phosphorylated MFGM proteins were protein transport and actin cytoskeleton organization. Gained data are discussed with regard to lipid secretory mechanisms in the MECs. All MS data have been deposited in the ProteomeXchange with identifier PXD001039 (http://proteomecentral.proteomexchange.org/dataset/PXD001039).

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Section: Discussionmentioning

confidence: 74%

Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell

Henry¹,

Saadaoui

Bouvier³

et al. 2015

Proteomics

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“…The first evidence of the effect of this genetic polymorphism on milk fat secretion arises from transcriptomic studies on mammary tissues. The expression levels of key lipogenic genes as well as genes known to influence membrane fluidity, cell-cell interaction, or chromatin organization are affected by the α s1 -CN genetic polymorphism in the goat (Ollier et al, 2008). Moreover, a study comparing FA profiles in goats displaying strong (A, B, and C alleles), or weak (F and G alleles) genotypes at the CSN1S1 locus demonstrated differences in proportions in milk fat of 17 FA, including the C8 to C12 SFA and palmitic, stearic, oleic, linoleic, and rumenic acids.…”

Section: Introductionmentioning

confidence: 99%

Goat αs1-casein genotype affects milk fat globule physicochemical properties and the composition of the milk fat globule membrane

Cebo

Lopez

Henry

et al. 2012

Journal of Dairy Science

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Milk fat secretion is a complex process that initiates in the endoplasmic reticulum of the mammary epithelial cell by the budding of lipid droplets. Lipid droplets are finally released as fat globules in milk enveloped by the apical plasma membrane of the mammary epithelial cell. The milk fat globule membrane (MFGM) thus comprises membrane-specific proteins and polar lipids (glycerophospholipids and sphingolipids) surrounding a core of neutral lipids (mainly triacylglycerols and cholesterol esters). We have recently described major proteins of the MFGM in the goat and we have highlighted prominent differences between goats and bovine species, especially regarding lactadherin, a major MFGM protein. Here, we show that, in the goat species, the well-documented genetic polymorphism at the α(s1)-casein (CSN1S1) locus affects both structure and composition of milk fat globules. We first evidenced that both milk fat globule size and ζ-potential are related to the α(s1)-casein genotype. At midlactation, goats displaying strong genotypes for α(s1)-casein (A/A goats) produce larger fat globules than goats with a null genotype at the CSN1S1 locus (O/O goats). A linear relationship (R(2)=0.75) between fat content (g/kg) in the milk and diameter of fat globules (μm) was established. Moreover, we found significant differences with regard to MFGM composition (including both polar lipids and MFGM proteins) from goats with extreme genotype at the CSN1S1 locus. At midlactation, the amount of polar lipids is significantly higher in the MFGM from goats with null genotypes for α(s1)-casein (O/O goats; 5.97±0.11mg/g of fat; mean ± standard deviation) than in the MFGM from goats with strong genotypes for α(s1)-casein (A/A goats; 3.96±0.12mg/g of fat; mean ± standard deviation). Two MFGM-associated proteins, namely lactadherin and stomatin, are also significantly upregulated in the MFGM from goats with null genotype for α(s1)-casein at early lactation. Our findings are discussed with regard to techno-functional properties and nutritional value of goat milk. In addition, the genetic polymorphism in the goat species appears to be a tool to provide clues to the lipid secretion pathways in the mammary epithelial cell.

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“…The breeding values of the animals were estimated based on milk yield. Therefore, these differences are very important as they affect CSN1S1 and BLG genotypes on transcriptional level, milk yield, and proteins that have been reported (2,33-35). In addition, the differential expression between breeding value groups may be caused by genetic variation that is related to breeding value groups.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of the Alpha S1 Casein and Beta-Lactoglobulin Genes in Different Physiological Stages of the Adani Goats Mammary Glands

Morammazi¹,

Masoudi²,

Torshizi³

et al. 2016

Iran. J. Biotechnol.

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Background Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired. Objectives In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed high and low breeding values. Materials and Methods The breeding values of the animals were estimated first by applying multi-trait random regression model. Using the biopsy gun, the mammary gland samples were taken and real-time PCR was applied to search the expression of the genes. Fixed factors of the model were the breeding value groups, sampling times and their interactions. Results The interactions were significant for both genes. At milking time, the high breeding value group exhibited more transcriptional levels for BLG and less transcriptional levels for CSN1S1 gene compared with the low breeding value group. The expression patterns of these genes were also different between the two breeding value groups. The maximum level of BLG and CSN1S1 transcriptions were found to occur at drying time. Conclusions A difference in the gene expression was observed between the two groups which indicate the change in the nucleotide sequence for transcription factor binding sites, or miRNA binding sites, otherwise in the coding regions. Therefore, the variations in the coding and promoter regions of this gene should be investigated in the further studies.

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Goat’s αS1-casein polymorphism affects gene expression profile of lactating mammary gland

Cited by 28 publications

References 36 publications

Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell

Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell

Goat αs1-casein genotype affects milk fat globule physicochemical properties and the composition of the milk fat globule membrane

Differential Expression of the Alpha S1 Casein and Beta-Lactoglobulin Genes in Different Physiological Stages of the Adani Goats Mammary Glands

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