2021
DOI: 10.1167/tvst.10.9.14
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Abstract: Purpose Compelling new evidence reveals a close link between the gut microbiome and the pathogenesis of neovascular age-related macular degeneration (nAMD). Germ-free (GF) animal models are the current gold standard for studying host the microbe interactions in vivo; yet, no GF animal models of nAMD are available today. This protocol describes gnotobiotic operations and assembly for a laser-induced choroidal neovascularization (CNV) model in GF mice to study the gut microbiome in neovascular AMD. … Show more

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Cited by 5 publications
(3 citation statements)
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“…Interestingly, when intestinal microbiota are altered in animal AMD models, either via antibiotic depletion, microbiota transplantation or diet alterations, AMD features are arrested or even reversed [14,15,30]. Using our germ-free CNV model, we found that mice devoid of microbiota are somewhat protected from nAMD [31 ▪ ], and develop smaller CNV lesions (unpublished data, Skondra laboratory). Biswas et al [32 ▪▪ ] show that efoxitine, a translocator protein (TSPO) activator, can potentially combat the effects of HFD-induced damage to the RPE byrestructuring the gut microbiome.…”
Section: Age-related Macular Degenerationmentioning
confidence: 68%
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“…Interestingly, when intestinal microbiota are altered in animal AMD models, either via antibiotic depletion, microbiota transplantation or diet alterations, AMD features are arrested or even reversed [14,15,30]. Using our germ-free CNV model, we found that mice devoid of microbiota are somewhat protected from nAMD [31 ▪ ], and develop smaller CNV lesions (unpublished data, Skondra laboratory). Biswas et al [32 ▪▪ ] show that efoxitine, a translocator protein (TSPO) activator, can potentially combat the effects of HFD-induced damage to the RPE byrestructuring the gut microbiome.…”
Section: Age-related Macular Degenerationmentioning
confidence: 68%
“…Germ-free and gnotobiotic animal models with specific microbial populations of interest are considered ideal to answer proof-of-concept questions of causal relationships of the gut microbiome and diseases [43,44]. Our team has described detailed protocols for performing sterile mouse procedures and creating germ-free and gnotobiotic animal retinal diseases models our germ-free nAMD model, a unique and valuable tool to investigate host-microbial interactions in nAMD [31 ▪ ]. Although some associative studies have been performed over the last decade, we have only recently begun to explore the retinal transcriptional signatures linking the microbial organ and retinal homeostasis and physiology relating to AMD.…”
Section: Age-related Macular Degenerationmentioning
confidence: 99%
“…Mice were lasered at an average age of 16 weeks for both groups. The GF lasering was performed under sterile conditions inside of a laminar flow hood, and all mice were sacrificed by CO 2 inhalation one week after the laser session, as described in detail in our paper [ 54 ]. After the procedure, GF mice were placed in hermetically sealed, ventilated cages and kept under sterile conditions.…”
Section: Methodsmentioning
confidence: 99%