Glycosylphosphatidylinositol-anchoring is required for the proper transport and glycosylation of classical arabinogalactan protein precursor in tobacco BY-2 cells
Abstract:Arabinogalactan proteins (AGPs) are extracellular proteoglycans with many O-linked glycan chains. Precursors to many AGPs contain a C-terminal signal for the addition of a GPI-anchor, yet the role of this modification has not been elucidated. NtAGP1, a tobacco precursor to AGP, comprises a signal peptide, an AGP-coding region and a GPI-anchoring signal, and classified as a member of classical AGP. Using green fluorescent protein (GFP) and sweet potato sporamin (SPO) as tags and tobacco BY-2 cells as the host, … Show more
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