Glycosylation of proteins of human skin fibroblasts is changed by rosmarinic acid

Abstract

Glycosylation is a common post-translational process of protein modification. Glycans participate in many crucial biological functions like cell differentiation, cell adhesion, cell-cell interactions, and regulation of signaling pathways. Rosmarinic acid (RA) is a natural flavonoid with many pharmacological activities including anti-inflammatory, anti-oxidative, anti-bacterial, or anti-fibrotic. In this study, we aimed to determine the effect of 25, 50, and 100 μM rosmarinic acid on specific carbohydrate antigens in human skin fibroblasts. ELISA-liked test with biotinylated lectins was used to assess the level of sugar structures in cell lysates and culture supernatant. RT-PCR was applied to determine mRNA of selected glycosyltransferases responsible for formation of sugar antigens. Rosmarinic acid inhibited the expression of Tn, T antigens and their sialylated forms, fucosylated antigens, di NAclactosamine, and mannose antigens. All used doses of RA significantly decreased core 1 β1-3galactosyltransferase mRNA and 25 and 50 μM acid significantly inhibited GalNAcα2-6-sialyltransferase mRNA. The results indicate that rosmarinic acid, due to decreasing effect on specific sugar antigens, can change some of crucial carbohydrate functions in skin fibroblasts, e.g., involved in cell adhesion and cell-cell interactions.

An integrative analysis to predict the Active Ingredients and explore Polypharmacological Mechanisms of Orthosiphon stamineus Benth. for human health

“…However, the water solubility of the Orthosiphol A is poor, and the gastrointestinal absorption is low in our ADMET model. Different from Orthosiphol A, Rosmarinic acid, a water-soluble and absorbable polyphenol that been researched endlessly, had several exciting effects, including antioxidant(Shen et al, 2017), anti-inflammatory(Luo et al, 2020), anti-tumor(Kim et al, 2015), antimicrobial(Slobodnikova et al, 2013), anti-diabetic(Ngo et al, 2018), neuroprotective(Ghasemzadeh and Hosseinzadeh, 2020; Hasanein and Mahtaj, 2015) and nephroprotective activities(Domitrovic et al, 2014; Al-Dhabi et al, 2014) even cosmetic effects(Iwona and Katarzyna, 2020). Similarly, two flavonoids Eupatorin and Sinensetin also been confirmed to possess almost the same pharmacological effects as O. aristatus (Han et al, 2020; Laavola et al, 2012; Chung et al, 2020; Ashraf et al, 2018).…”

“…However, the water solubility of the Orthosiphol A is poor, and the gastrointestinal absorption is low in our ADMET model. Different from Orthosiphol A, Rosmarinic acid, a water-soluble and absorbable polyphenol that been researched endlessly, had several exciting effects, including antioxidant 35 , anti-inflammatory 36 , anti-tumor 37 , antimicrobial 38 , anti-diabetic 39 , neuroprotective 40,41 and nephroprotective activities 42,43 even cosmetic effects 44 . Similarly, two flavonoids Eupatorin and Sinensetin also been confirmed to possess almost the same pharmacological effects as O. aristatus 4,[45][46][47] .…”

The Effects of Rosmarinic Acid on Expression of Akt Genes in the Hippocampus of Diabetic Rats and DNA Glycation Inhibition.

“…This fructose concentration correlates to the minimum concentration needed for in vitro DNA glycation 49 . It has also been shown that 25 μM rosmarinic acid reduces the glycosylation of human broblasts in vitro 50 . 0.02 % (w/v) NaN3 was added to the solution and puri ed into a low proteinbinding lter (-GV 0.22 µm lter unit, Millipore) to avoid bacterial contamination.…”

Rosmarinic acid inhibits DNA glycation and modulates the expression of Akt1 and Akt3 partially in the hippocampus of diabetic rats

“…This fructose concentration correlates to the minimum concentration needed for in vitro DNA glycation 49 . It has also been shown that 25 µM rosmarinic acid reduces the glycosylation of human fibroblasts in vitro 50 . 0.02% (w/v) NaN3 was added to the solution and purified into a low protein-binding filter (-GV 0.22 µm filter unit, Millipore) to avoid bacterial contamination.…”