Glycodendritic Structures Based on Boltorn Hyperbranched Polymers and Their Interactions with Lens culinaris Lectin

Abstract

Multivalent scaffolds bearing carbohydrates have been prepared to mediate biological processes where carbohydrates are involved. These systems consist of dendritic structures based on Boltorn H20 and H30 hyperbranched polymers to which carbohydrates are linked through a convenient spacer. Mannose has been chosen as a sugar unit to test the viability of this strategy. These glycodendritic compounds have been prepared in a few steps with good yields, showing a high solubility in physiological media and low toxicity. The binding of these dendritic polymers to the mannose-binding lectin Lens culinaris (LCA) was studied using STD-NMR experiments and quantitative precipitation assays. The results demonstrate the existence of a clear interaction between the mannose derivative systems and the Lens lectin where the dendritic scaffold does not have an important role in mannose binding but supplies the necessary multivalence for lectin cluster formation. These glycodendritic structures are able to interact with a receptor, and therefore they can be considered as promising tools for biological studies.

Gold Manno‐Glyconanoparticles: Multivalent Systems to Block HIV‐1 gp120 Binding to the Lectin DC‐SIGN

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A multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus

“…This approach has the potential to afford multivalent DC-SIGN antagonists of high potency, that also display improved accessibility and chemical stability over previously studied materials. Their DC-SIGN binding properties could be studied in Surface Plasmon Resonance (SPR) and in infection cellular models, using azidofunctionalized mannose 5 [23], the pseudo-trisaccharide 2b [24] and pseudo-disaccharide 3b [25] to synthesize appropriate controls.…”

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