Glycerol degradation in the thermoacidophilic crenarchaeon<i>Sulfolobus acidocaldarius</i>involves an unusual glycerol-3-phosphate dehydrogenase

Schmerling, Christian; Schroeder, Carsten; Zhou, Xiaoxiao; Busche, Tobias; Kalinowski, Jörn; Montero, Lidia; Schmitz, Oliver; Kaschani, Farnusch; Ninck, Sabrina; Kaiser, Markus; Bost, Jan; Waßmer, Bianca; Albers, Sonja V.; Siebers, Bettina; Bräsen, Christopher

doi:10.1101/2024.02.29.582781

Cited by 1 publication

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“…S. acidocaldarius strains MW001 (uracil auxotrophic) (31) and MW001 adapted to growth on glycerol designated herein MW00G (32) were cultured aerobically (Innova®44, New Brunswick, Germany) in Brock´s basal medium at 75°C, pH 3, 180 rpm (33) supplemented with 10 µg/ml of uracil as well as 0.1% (w/v) N-Z-amine and 0.3% (w/v) D-xylose (for induction of homologous overexpression). The Gelrite solidified Brock medium used for S. acidocaldarius MW001 and MW00G based cloning and transformation procedures was prepared as mentioned before (31).…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

De novosynthesis of fatty acids in Archaea via an archaeal fatty acid synthase complex

Schmerling,

Zhou,

Görs

et al. 2024

Preprint

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Archaea synthesize membranes using isoprenoid-based ether lipids, whereas Bacteria and Eukarya usefattyacid-based ester lipids. While the factors responsible for this “lipid divide” remain unclear, this has important implications for understanding the evolutionary history of eukaryotes, which likely originated from within the Archaea and therefore changed membrane composition from isoprenoid-based to fatty acid-based lipids. Here, using13C labelling studies, we demonstrate that the archaeal model organismsSulfolobus acidocaldariusandHaloferax volcaniiare capable ofde novofatty acid synthesis. Biochemical characterization andin vitropathway reconstitution identify the key enzymes of a newly proposed fatty acid synthesis pathway inS. acidocaldariusand show that ketothiolase, ketoacyl-CoA reductase, and hydroxyacyl-CoA dehydratase form a stable assembly mediated by a DUF35 domain protein, which represents the first characterization of an archaeal fatty acid synthase complex. The final step is catalysed by an NADPH-dependent enoyl-CoA reductase. Deletion of the enoyl-CoA reductase demonstrate that this pathway operatesin vivoin S. acidocaldarius. The presented results including phylogenetic analysis reveal that the potential to synthesize fatty acids is widespread across archaeal lineages. Collectively, our findings demonstrate that archaea are capable of synthesizing fatty acids, elucidate the molecular mechanisms involved in this process and provide additional insights into the evolutionary histories of fatty acid synthesis in archaea.

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Section: Strains and Growth Conditionsmentioning

confidence: 99%