Glutamic-Oxaloacetic Transaminase, Glutamic-Pyruvic Transaminase, and Lactic Dehydrogenase in the Jejunum

Abstract

Experiments in which isolated pig intestines were used showed the following : (1) GOT, GPT, and LDH are demonstrable in abundance in the liquid recovered from intestines which have been exposed to the action of 50 ml of a 50% glucose solution for 15 min following washings with isotonic glucose solutions. (2) If intestines were exposed to a 50% glucose solution for 4—6 consecutive 15- minute-periods the concentration of enzymes in the liquid recovered would fall. (3) Enzyme electrophoretic investigations of LDH in plasma, in the intestinal content, and in the mucous membrane of the intestine, indicate that the LDH present in the intestinal liquid originates from the intestinal mucous membrane. (4) The presence of GOT, GPT, and LDH in the intestinal liquid may be considered a secretion or diffusion of these enzymes into the intestinal lumen and not exclusively as indicators of cell damage.

The Electrolyte and Protein Concentration in Hypertonic Glucose Solutions 15 Minutes after Intra-Intestinal Injection into Small Intestinal Sections of Varying Lengths

“…These enzymes displayed very specific phenomena which have been de scribed in another place (Marner et al [11]) but the experiments failed to substantiate such a theory of a severe damage of tissues.…”