Glucosyltransferase Mutants of
            <i>Leuconostoc mesenteroides</i>
            NRRL B-1355

Smith, M. R.; Zahnley, James C.; Goodman, Nelson

doi:10.1128/aem.60.8.2723-2731.1994

Cited by 34 publications

(29 citation statements)

References 26 publications

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“…Dextran production is influenced by a number of factors: physical and chemical properties (Jeanes, 1965), such as solubility, viscosity, specific optical rotation and the content of nitrogen, phosphorus and ash in the medium, which further depends on the specific microorganisms involved (Jeanes et al, 1954). A single enzyme can catalyze the synthesis of several types of dextran linkages, thereby permitting the formation of a branched polymer (Neely & Nott, 1962;Smith, Sahnley, & Goodman, 1994). On the other hand, certain bacterial strains have been shown to produce dextrans of different structures, which have been attributed to the excretion of different dextransucrases (Côté & Robyt, 1982;Figures & Edwards, 1981;Zahley & Smith, 1995).…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Vettori¹,

Franchetti²,

Contiero³

2012

Carbohydrate Polymers

107

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This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1 H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% ␣-(1,6) linkages in the main chains and 2.1% ␣-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% ␣-(1,6) linkages in the main chains and 5% ␣-(1,3) branch linkages.

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Section: Introductionmentioning

confidence: 99%

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Vettori¹,

Franchetti²,

Contiero³

2012

Carbohydrate Polymers

107

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show abstract

“…The frequency and nature of the branch points mainly depend on the origin of the dextransucrase (i.e., the producing microorganism) (9). A single enzyme can catalyze the synthesis of several types of linkages, thus permitting, on its own, the formation of a branched polymer (20,31). On the other hand, certain bacterial strains have been shown to produce dextrans of various structures, and this was attributed to the excretion by the microorganism of different dextransucrases (1,5,33).…”

mentioning

confidence: 99%

Characterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium by Leuconostoc mesenteroides NRRL B-1299

Dols¹,

Remaud‐Siméon²,

Willemot³

et al. 1998

Appl Environ Microbiol

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When grown in glucose or fructose medium in the absence of sucrose,Leuconostoc mesenteroides NRRL B-1299 produces two distinct extracellular dextransucrases named glucose glucosyltransferase (GGT) and fructose glucosyltransferase (FGT). The production level of GGT and FGT is 10 to 20 times lower than that of the extracellular dextransucrase sucrose glucosyltransferase (SGT) produced on sucrose medium (traditional culture conditions). GGT and FGT were concentrated by ultrafiltration before sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Their molecular masses were 183 and 186 kDa, respectively, differing from the 195 kDa of SGT. The structural analysis of the dextran produced from sucrose and of the oligosaccharides synthesized by acceptor reaction in the presence of maltose showed that GGT and FGT are two different enzymes not previously described for this strain. The polymer synthesized by GGT contains 30% α(1→2) linkages, while FGT catalyzes the synthesis of a linear dextran only composed of α(1→6) linkages.

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“…For this reason, it was classified under a reference number different from the number used for all other GTFs and received the reference EC 2.4.1.140. When incubated in the presence of a sugar acceptor like maltose, ASR keeps its specificity and catalyzes the formation of oligoalternans [3–5]. Whereas many genes encoding either dextransucrases or mutansucrases are available, no genes encoding ASR have been previously reported.…”

Section: Introductionmentioning

confidence: 99%

Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase fromLeuconostoc mesenteroidesNRRL B-1355

Argüello-Morales

Remaud‐Siméon

Pizzut

et al. 2000

FEMS Microbiology Letters

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The gene encoding alternansucrase (ASR) from Leuconostoc mesenteroides NRRL B-1355, an original sucrose glucosyltransferase (GTF) specific to alternating alpha-1,3 and alpha-1,6 glucosidic bond synthesis, was cloned, sequenced and expressed into Escherichia coli. Recombinant enzyme catalyzed oligoalternan synthesis from sucrose and maltose acceptor. From sequence comparison, it appears that ASR possesses the same domains as those described for GTFs specific to either contiguous alpha-1,3 osidic bond or contiguous alpha-1,6 osidic bond synthesis. However, the variable region and the glucan binding domain are longer than in other GTFs (by 100 and 200 amino acids respectively). The N-catalytic domain which presents 49% identity with the other GTFs from L. mesenteroides possesses the three determinants potentially involved in the glucosyl enzyme formation.

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Glucosyltransferase Mutants of Leuconostoc mesenteroides NRRL B-1355

Abstract: Leuconostoc mesenteroides NRRL B-1355 produces dextrans and alternan from sucrose. Alternan is an unusual dextran-like polymer containing alternating a(1->6)/a(1-*3) glucosidic bonds. Cultures were

Cited by 34 publications

References 26 publications

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Characterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium by Leuconostoc mesenteroides NRRL B-1299

Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase fromLeuconostoc mesenteroidesNRRL B-1355

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