Glucose is required to maintain ATP/ADP ratio of isolated bovine cerebral microvessels

Abstract

Isolated bovine cerebral microvessels (ICMV) were incubated with different metabolic fuels to determine the effect of each of them on microvessel energy state. With no fuel added to the medium, the ATP/ADP generally decreased from initial values of 1.5-3 down to 1-1.5 over 4 h; the ATP content also declined approximately 50%. In contrast, with glucose present, the ATP/ADP increased, and the ATP content was maintained. Pyruvate, beta-hydroxybutyrate, glutamate, and oleate were ineffective; oleate added together with carnitine gave some improvement but less than with glucose. Oxygen consumption by ICMV did not differ appreciably in fuel-free or glucose-containing medium. Addition of an inhibitor of fatty acid oxidation, 2-tetradecylglycidate, depressed the ATP/ADP. These results suggest that ICMV require glycolysis to maintain both their content of ATP and their ATP/ADP. They also suggest that endogenous lipid is an important fuel for isolated microvessels.

Effects of negative pressure wound therapy on cellular energetics in fibroblasts grown in a provisional wound (fibrin) matrix

“…The number of mitochondria present in fibroblasts may change, depending upon the metabolic requirements of the cell 13,14 . Because the majority of ATP production in eukaryotic cells takes place in the mitochondria (which can make up nearly 25% of the total volume of a cell), increasing mitochondrial numbers, areas, or volumes, may also be indicators of a higher capacity for OxPhos 15 . Cytochrome c oxidase levels have also been shown to influence ATP synthesis in fibroblasts 16 .…”

Atrial Natriuretic Peptide Regulates Ca2+ Channel in Early Developmental Cardiomyocytes

“…Other reports support the notion that ANP has no direct effect on action potential duration (APD) or contractility of isolated guinea pig ventricular papillary muscle [36]–[38]. While ANP may not have a direct negative chronotropic effect, the receptor linkage to the guanylyl cyclase in rabbit atrium [39], rabbit [40] and rat ventricle [41], [42], as well as rabbit Purkinje fibers [43], [44] has been proven. Moreover, ANP receptor stimulation results in an increase of intracellular cGMP and thereafter a decrease of cAMP levels [14].…”