Glucose capped silver nanoparticles induce cell cycle arrest in HeLa cells

Panzarini, Elisa; Mariano, Stefania; Vergallo, Cristian; Carata, Elisabetta; Fimia, Gian María; Mura, Francesco; Rossi, M.; Vergaro, Viviana; Ciccarella, Giuseppe; Corazzari, Marco

doi:10.1016/j.tiv.2017.02.014

Cited by 52 publications

(39 citation statements)

References 43 publications

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“…ZnO NPs dose and time dependency was shown by Ahamed et al who reported ZnO NP upregulation of BAX and downregulation of BIRC5/survivin and BCL2 in lung cancer cells (A549 cells) [134]. Ag NPs induce oxidative stress resulting in genotoxicity in a TP53/p53-dependent manner in a variety of experimental systems such as adult cells, stem cells and cancer cells [135][136][137][138].…”

Section: Apoptosis Triggered By Npsmentioning

confidence: 91%

Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles

et al. 2018

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Nanomaterials have gained a rapid increase in use in a variety of applications that pertain to many aspects of human life. The majority of these innovations are centered on medical applications and a range of industrial and environmental uses ranging from electronics to environmental remediation. Despite the advantages of NPs, the knowledge of their toxicological behavior and their interactions with the cellular machinery that determines cell fate is extremely limited. This review is an attempt to summarize and increase our understanding of the mechanistic basis of nanomaterial interactions with the cellular machinery that governs cell fate and activity. We review the mechanisms of NP-induced necrosis, apoptosis and autophagy and potential implications of these pathways in nanomaterial-induced outcomes. Abbreviations: Ag, silver; CdTe, cadmium telluride; CNTs, carbon nanotubes; EC, endothelial cell; GFP, green fluorescent protein; GO, graphene oxide; GSH, glutathione; HUVECs, human umbilical vein endothelial cells; NP, nanoparticle; PEI, polyethylenimine; PVP, polyvinylpyrrolidone; QD, quantum dot; ROS, reactive oxygen species; SiO, silicon dioxide; SPIONs, superparamagnetic iron oxide nanoparticles; SWCNT, single-walled carbon nanotubes; TiO, titanium dioxide; USPION, ultra-small super paramagnetic iron oxide; ZnO, zinc oxide.

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Section: Apoptosis Triggered By Npsmentioning

confidence: 91%

Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles

et al. 2018

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“…In our experiments, biotoxicity is not due to the Ag + ions release or the nanoparticle aggregates. To reduce as much as possible the Ag + release we used β-D-glucose for the green chemistry synthesis of AgNPs on the base of our previous data indicating that AgNPs-G are stable, well-dispersed with a minimum Ag + release in culture medium [30]. Indeed, in our experiments we measured the release of only 5% of the amount of the NPs after 10 days in seawater, thus con rming the effectiveness of the synthesis based on β-D-glucose as a reducing agent.…”

Section: Discussionmentioning

confidence: 99%

“…However, studies on the ability of microalgae to remedy the NPs aquatic pollution are still very limited [28]. In this study, we used green chemistry synthesized AgNPs, that were functionalised with Glucose-G (AgNPs-G) to ensure AgNPs stability [29,30,31] and Chlorella vulgaris, one of the most widely used microorganism in testing NPs/NMs effects on aquatic biota but also known to reduce heavy metals from waters. To exploit the ability of C. vulgaris in the removal of AgNPs-G from water, we investigated the e ciency of the microalgae to uptake and retain NPs.…”

Section: Introductionmentioning

confidence: 99%

Toxicity, bioaccumulation and biotransformation of glucose capped silver nanoparticles in green microalgae Chlorella vulgaris

Mariano

Panzarini

Inverno

et al. 2020

Preprint

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BackgroundSilver nanoparticles (AgNPs) are one of the most widely used nanomaterials in consumer products. When discharged into the aquatic environment AgNPs can cause toxicity to aquatic biota, through mechanisms that are still under debate, thus rendering the NPs effects evaluation a necessary step. Different aquatic organism models, i.e. microalgae, mussels, Daphnia magna, sea urchins and Danio rerio, etc. have been largely exploited for NPs toxicity assessment. On the other hand, alternative biological microorganisms abundantly present in nature, i.e. microalgae, are nowadays exploited as a potential sink for removal of toxic substances from the environment. Indeed, the green microalgae Chlorella vulgaris is one of the most used microorganisms for waste treatment.ResultsWith the aim to verify the possible involvement of C. vulgaris not only as a model microorganism of NPs toxicity but also for the protection toward NPs pollution, we used these microalgae to measure the AgNPs biotoxicity and bioaccumulation. In particular, to exclude any toxicity derived by Ag+ ions release, green chemistry synthesised and Glucose coated AgNPs (AgNPs-G) were used. C. vulgaris actively internalised AgNPs-G whose amount increases in a time and dose-dependent manner. The internalised NPs, found inside large vacuoles, were not released back into the medium, even after 1 week, and did not undergo biotransformation since AgNPs-G maintained their crystalline nature. Biotoxicity of AgNPs-G causes an exposure time and AgNPs-G dose-dependent growth reduction and a decrease in chlorophyll-a amount.ConclusionsThese results confirm C. vulgaris as a biomonitoring organism and also suggest it as a bioaccumulating microalgae for possible use in the environment protection.

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“…Here, P53 acts as the guardian of the genome and is encoded by the TP53 gene. This gene acts as a tumor suppressor and is either mutated or inactive in half of all cancer cells [52,56,85,103]. Accordingly, we also know that decreased Bcl-2 expression is influenced by the JNK pathway.…”

Section: Mechanisms Of Proteogenomic Signal Specificity and Apoptosismentioning

confidence: 99%

Microcellular Environmental Regulation of Silver Nanoparticles in Cancer Therapy: A Critical Review

Ganesan

Jang

Suh

et al. 2020

Cancers

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Silver nanoparticles (AgNPs) play significant roles in various cancer cells such as functional heterogeneity, microenvironmental differences, and reversible changes in cell properties (e.g., chemotherapy). There is a lack of targets for processes involved in tumor cellular heterogeneity, such as metabolic clampdown, cytotoxicity, and genotoxicity, which hinders microenvironmental biology. Proteogenomics and chemical metabolomics are important tools that can be used to study proteins/genes and metabolites in cells, respectively. Chemical metabolomics have many advantages over genomics, transcriptomics, and proteomics in anticancer therapy. However, recent studies with AgNPs have revealed considerable genomic and proteomic changes, particularly in genes involved in tumor suppression, apoptosis, and oxidative stress. Metabolites interact biochemically with energy storage, neurotransmitters, and antioxidant defense systems. Mechanobiological studies of AgNPs in cancer metabolomics suggest that AgNPs may be promising tools that can be exploited to develop more robust and effective adaptive anticancer therapies. Herein, we present a proof-of-concept review for AgNPs-based proteogenomics and chemical metabolomics from various tumor cells with the help of several technologies, suggesting their promising use as drug carriers for cancer therapy.

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Glucose capped silver nanoparticles induce cell cycle arrest in HeLa cells

Cited by 52 publications

References 43 publications

Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles

Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles

Toxicity, bioaccumulation and biotransformation of glucose capped silver nanoparticles in green microalgae Chlorella vulgaris

Microcellular Environmental Regulation of Silver Nanoparticles in Cancer Therapy: A Critical Review

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