Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes

Devasenathipathy, Rajkumar; Mani, Veerappan; Chen, Shen‐Ming; Huang, Sheng‐Tung; Huang, Tsung Tao; Lin, Chun Mao; Hwa, Kuo-Yuan; Chen, Ting Yo; Chen, Bo Jun

doi:10.1016/j.enzmictec.2015.06.006

Cited by 113 publications

(40 citation statements)

References 30 publications

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“…Numerous studies (Talbert et al, 2014;Wong et al, 2008;Choi et al, 2015;Singh and Verma, 2013;Röcker et al, 2016) have shown increase in the traditional uses of this enzyme. In addition, GOx has more recently been used in biosensor applications (Devasenathipathy et al, 2015;Turkmen et al, 2014;Hwa, 2015;Lin et al, 2015) and other nanotechnological applications have been demonstrated Liu et al, 2014;Sapountzi et al, 2017). In this context, the new Amazonian source of this enzyme (A. niger LMM01) and production of GOx in a semi-continuous process demonstrates the importance of the present work.…”

Section: Discussionmentioning

confidence: 91%

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Sousa¹,

Santos²,

Cortez³

et al. 2017

Afr. J. Microbiol. Res.

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Glucose oxidase (GOx) has several industrial applications. It is believed that there are several species of fungi that have the ability to produce this enzyme, most of which are unexplored. This work aimed to investigate the production of glucose oxidase (EC 1.1.3.4) by fungi isolated from soil samples of the Amazonian forest. Filamentous fungi were isolated from soil samples from the Adolpho Ducke Forest Reserve, located in Manaus, Amazonas. Strains were subjected to submerged bioprocessing to select for the best GOx producers. Those selected for the production of isolated enzymes were subjected to kinetic tests that evaluated production of the enzyme and consumption of the biomass substrate by the isolates. In addition, experiments to evaluate the optimal carbon, nitrogen and phosphorus sources as well as the influence of the bioprocess factors were carried out. Finally, GOx production was investigated in a semi-continuous system for 7 days. The most frequent isolates isolated from soil samples belonged to the genera Aspergillus, Penicillium and Trichoderma. Aspergillus niger LMM01 was the best GOx producer. Glucose, peptone and KH 2 PO 4 were demonstrated to be the optimal carbon, nitrogen and phosphorus sources, respectively. Multivariate experiments demonstrated that the parameters with the greatest effect on GOx production were pH and agitation. Stable expression results for GOx (7.74 U/ml) were obtained over 7 days in a semi-continuous process. In this context, the new Amazonian source of this enzyme (A. niger LMM01), and enzyme production in a semi-continuous process, demonstrates the importance of the present work.

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Section: Discussionmentioning

confidence: 91%

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Sousa¹,

Santos²,

Cortez³

et al. 2017

Afr. J. Microbiol. Res.

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“…Our electrode shows sensitivity and LOD similar to results by Palanisamy et al 18 which used thick GO hybridized with MWCNT and supported by glassy carbon electrode (GCE) that demonstrated high thermodynamic surface adsorption 59 and specific thermodynamics adsorption and redox reduction mechanism with GOx. 60 Although our electrode underperformed those with thick RGO, 11,18,28,29,53 it demonstrated the feasibility of immobilizing GOx onto monolayer RGO for glucose sensors. The LOD of our electrodes outperformed those reported previously for GOx immobilized hybrids of AuNPs-RGO 56 and CdS-RGO 57 on GCE and RGO-IL on modified Au.…”

Section: Resultsmentioning

confidence: 99%

“…The sensitivity and LOD of the fabricated electrode are compared to various electrodes as shown in Table II. 11,18,28,29,[53][54][55][56][57][58] Most of the electrodes in the review have high sensitivity and good LOD as they used thick GO prepared by a modified Hummers method. Our electrode shows sensitivity and LOD similar to results by Palanisamy et al 18 which used thick GO hybridized with MWCNT and supported by glassy carbon electrode (GCE) that demonstrated high thermodynamic surface adsorption 59 and specific thermodynamics adsorption and redox reduction mechanism with GOx.…”

Section: Resultsmentioning

confidence: 99%

“…24 GO sheets synthesized from graphite powders by the modified Hummers method 25 have been coated on carbon and Pt electrodes and used to immobilize GOx to develop glucose sensors. [26][27][28][29] On the other hand, monolayer graphene has been fabricated by chemical vapor deposition (CVD) on Cu and Ni substrates then transferred to other substrates for chemical and gas sensors. [30][31][32] Monolayer graphene has also been utilized for glucose sensor after immobilizing GOx to its surface through linker molecules.…”

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confidence: 99%

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Functionalization of CVD Grown Graphene with Downstream Oxygen Plasma Treatment for Glucose Sensors

Hadish

Jou

Huang

et al. 2017

J. Electrochem. Soc.

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The development of graphene-based devices requires control over the functionalization and modification of the graphene surface. We studied the modification of the monolayer graphene surface with downstream oxygen plasma treatment to form graphene oxide (GO) for use in glucose biosensors. Amperometric and voltammetric methods were applied to GO for glucose oxidase (GOx) immobilization with a simultaneous reduction of GO to reduce graphene oxide. The fabricated glucose sensor shows a sensitivity of 0.118 μA mM −1 cm −2 and a detection limit of 0.0526 mM of glucose. This method provides fast and simultaneous immobilization of GOx and reduction of GO, and can be used in the fabrication of other electrochemical biosensors. Glucose oxidase has been recognized as an enzyme for monitoring glucose. In amperometric biosensors for glucose detection, GOx is immobilized on a nanomaterial surface and catalytically oxidizes glucose for high sensitivity and selectivity.9-11 However, enzyme immobilization is complicated by poor electrical communication of the active sites of the enzyme and the electrode surface, and by enzyme leaching. [12][13][14][15] Recently, graphene has been used to modify biosensors, offering advantages for enzyme immobilization on graphene surfaces. [16][17][18] Pristine graphene (PrG), a monolayer structure composed of one-atom thick two-dimensional honeycomb lattices of sp 2 carbon atoms, has extraordinary properties such as high intrinsic mobility and excellent thermal and electrical conductivity. [19][20][21][22] Although the bulk properties of graphene are very promising for a variety of applications, the development of graphene-based devices requires great control over the functionalization and modification of the graphene surface. Graphene oxide (GO) is one branch of functionalized graphene. GO is typically covalently functionalized with hydroxyl, epoxy, carbonyl, and carboxyl groups on its basal planes and at its edges, 23 resulting in a hybrid structure comprising a mixture of sp 2 and sp 3 hybridized carbon atoms. 24 GO sheets synthesized from graphite powders by the modified Hummers method 25 have been coated on carbon and Pt electrodes and used to immobilize GOx to develop glucose sensors. [26][27][28][29] On the other hand, monolayer graphene has been fabricated by chemical vapor deposition (CVD) on Cu and Ni substrates then transferred to other substrates for chemical and gas sensors. [30][31][32] Monolayer graphene has also been utilized for glucose sensor after immobilizing GOx to its surface through linker molecules. 33,34 With the aid of lithography and plasma etch techniques, monolayer-graphene-based devices can be patterned into arrays 32 and integrated with Si microelectronics technology. 35,36 In this work we synthesized GO from monolayer graphene by a downstream oxygen plasma. 37,38 The plasma-assisted technique has the advantages of short treatment time, an environmentally friendly manufacturing process, and the generation of numerous active species. 39 The immobilization of GOx en...

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“…An innovative glucose biosensor, developed by Devasenathipathy et al, 30 involved the coupling of biopolymer pectin-stabilized AuNPs electrodeposited on graphene and multiwalled carbon nanotubes (GR-MWCNTs/AuNPs) as shown in Figure 4. The biosensor was prepared by adding multiwalled carbon nanotubes (MWCNTs) in graphite oxide (GO) and ultrasonicating them to obtain GO-MWCNTs.…”

mentioning

confidence: 99%

Nanomaterial-based biosensors for biological detections

Su¹,

Li²,

Jin³

et al. 2017

AHCT

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Nanomaterial-based biosensors have become one of the major topics in the field of diagnostics. With the growing demand on devices with improved sensitivity and selectivity, rapid response time, and low cost, four categories of nanomaterials have become popular in biosensor research: gold nanoparticles, graphene, carbon nanotubes, and photonic crystals. The ongoing research has brought new designs of biosensors based on nanomaterials, which have greatly improved the potential of field-deployable microfabricated devices. This review describes the recent technologies employing the aforementioned nanomaterials for electrochemical detection of biomolecules, including glucose, DNA, protein, toxins, and so on. We envisage that miniaturized lab-on-a-chip devices employing these nanomaterials will soon be an essential part of our daily life.

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Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes

Cited by 113 publications

References 30 publications

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Aspergillus niger LMM01: A new source of glucose oxidase in Amazon

Functionalization of CVD Grown Graphene with Downstream Oxygen Plasma Treatment for Glucose Sensors

Nanomaterial-based biosensors for biological detections

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