Global quantitative analysis of protein expression and phosphorylation status in the liver of the medaka fish (Oryzias latipes) exposed to microcystin-LR

A proteomic analysis of prenatal transfer of microcystin-LR induced neurotoxicity in rat offspring

“…Recently, it has been reported that MCLR triggered reorganization of microtubule and actin cytoskeleton components, leading to a loss of their filamentous distribution [35,36]. In agreement with the previous findings, our study demonstrated that MCLR could induce varied expression of the cytoskeleton and its associated proteins in rat brain, indicating that the neurotoxicity of MCLR is involved in disruption of neuronal cytoskeletal architecture.…”

“…Consistent with this, the ultrastructure of the cerebrum and levels of MDA, GSH and AChE activity were detected to be changed in MCLR-treated neonatal rats in our study. We found that the ultrastructure of cerebrum was conspicuously changed by MCLR which is in agreement with the prior findings [35,36], especially the endoplasmic reticulum and mitochondria which may play vital roles in the ER stress and oxidative stress. MCLR was found to inhibit antioxidant GSH level and MDA was increased which indicated that oxidative stress occurs in the brains.…”

Review of recent proteomic applications in aquatic toxicology

“…That study [6] was the first to apply this technique to aquatic toxicology and proved its utility and power. Proteomic research on non-model fish species have examined physiological responses to endocrine disrupting compounds [33,34], Cd [36,37], and microcystin-LR [7,38,39], among other environmental contaminants [30]. Protein profiles have mostly been evaluated in hepatic tissues, but occasionally in gill [37], brain [36], and kidney tissues [40].…”

Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels

“…Proteomics methods have been employed to study the effects of MC exposure in mice, medaka fish (Oryzia sltipes), adult zebrafish (Danio rerio), zebrafish embryos, and human amnion FL cells [12,[19][20][21][22][23]. The proteomics methods have been proved to be useful in identifying early responses to the MCs and, simultaneously identifying the biomarkers of toxicity involved in the effects of MCs on organism [19][20][21].…”

“…The proteomics methods have been proved to be useful in identifying early responses to the MCs and, simultaneously identifying the biomarkers of toxicity involved in the effects of MCs on organism [19][20][21]. The proteomics methods are used in the present study to understand the mechanism of immunotoxicity of MCLR in rats.…”