2011
DOI: 10.1038/nature10098
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Abstract: SummaryGene expression is a multistep process that involves transcription, translation and turnover of mRNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale. Here, we simultaneously measured mRNA and protein abundance and turnover by parallel metabolic pulse labeling for more than 5,000 genes in mammalian cells. While mRNA and protein levels correlated better than previously thought, corresponding half-lives showed … Show more

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Cited by 5,444 publications
(5,500 citation statements)
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References 48 publications
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“…To obtain quantitative information about a broader range of centrosomal proteins, we subjected the above centrosome preparations to shotgun proteomics and estimated protein abundance using the iBAQ method (Schwanhausser et al , 2011; Arike et al , 2012; Ahrne et al , 2013). Figure 3 and Table EV2 summarize the results for 73 core centrosomal proteins [including 58/61 proteins described in Azimzadeh et al (2012)].…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To obtain quantitative information about a broader range of centrosomal proteins, we subjected the above centrosome preparations to shotgun proteomics and estimated protein abundance using the iBAQ method (Schwanhausser et al , 2011; Arike et al , 2012; Ahrne et al , 2013). Figure 3 and Table EV2 summarize the results for 73 core centrosomal proteins [including 58/61 proteins described in Azimzadeh et al (2012)].…”
Section: Resultsmentioning
confidence: 99%
“…The database search results were filtered using the ion score to set the false discovery rate (FDR) to 1% on the peptide and protein level, respectively, based on the number of reverse protein sequence hits in the datasets. After normalizing the quantitative data of the technical replicates using the SafeQuant software tool (Glatter et al , 2012), the summed precursor intensities obtained from Progenesis for each centrosome preparation were aligned with the absolute protein concentrations determined by SRM analysis according to the iBAQ method (Schwanhausser et al , 2011), respectively. The resulting models were applied to estimate absolute protein levels for all proteins quantified in two centrosome preparations, and data were combined by calculating mean protein levels.…”
Section: Methodsmentioning
confidence: 99%
“…The concentration of specific proteins is not only the result of transcription, but also the balance between mRNA degradation, translation, and protein degradation (Schwanhäusser, Busse, & Li, 2011). While stress can alter translation (Picard, Loubière, Girbal, & Cocaign‐Bousquet, 2013), mRNA degradation (Huch & Nissan, 2014), protein degradation (Flick & Kaiser, 2012), and protein activity (Krebs & Holbrook, 2001; Pan et al., 2015), transcriptomic regulation nonetheless can show a clear pattern of response to environmental stressors and, thus, indicate the involvement of genes and physiological processes in the organism's response (de Nadal et al., 2011; Evans & Hofmann, 2012; Evans & Watson‐Wynn, 2014; Anderson et al., 2015).…”
Section: Discussionmentioning
confidence: 99%
“…Raw MS files from QConCat Mlp1 immunoisolation experiments were analyzed by the MaxQuant iBAQ method 64 . Only non-isotopically labeled (not QConCat) peptides were considered.…”
Section: Methodsmentioning
confidence: 99%