Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation

Yao, Pei-Li; Tsai, Meng‐Feng; Lin, Ying-Zu; Wang, Chien-Hsun; Liao, Wen-Chieh; Chen, Jeremy J.W.

doi:10.1186/1465-9921-6-89

Cited by 21 publications

(14 citation statements)

References 41 publications

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“…A number of mediators released from macrophages that play an important role in asthmatic inflammation are implied for their involvement either in the beginning or persisting of inflammation [25,26]. IL-1β, IL-6, IL-8, platelet activating factor (PAF) and tumour necrosing factor α (TNFα) are markers released from macrophages and stimulate many inflammatory cells as well as the production of acute phase proteins [7,9,26]. This stimulation might be the cause of the augmentation of well known acute phase reactant SAA in bronchial tissue.…”

Section: Discussionmentioning

confidence: 99%

Serum amyloid A (SAA) in induced sputum of asthmatics: A new look to an old marker

Özşeker

Büyüköztürk

Depboylu

et al. 2006

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

Serum amyloid A (SAA) in induced sputum of asthmatics: A new look to an old marker

Özşeker

Büyüköztürk

Depboylu

et al. 2006

International Immunopharmacology

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“…The CM is a serumfree medium with 24 h incubation with phorbol myristate acetate-treated THP-1 cells. Our previous studies and the other reports showed that the CM might contain proinflammatory cytokines such as tumor necrosis factor-a, IL-1, IL-8, and IL-12; anti-inflammatory cytokines such as transforming growth factor-h, IL-4, IL-10, and IL-13; and IL-6 with both proinflammatory and anti-inflammatory properties (3,11,27,39,40). Previous reports have shown that the imbalance between proinflammatory and anti-inflammatory cytokines can influence neoplastic outcome (3).…”

Section: Discussionmentioning

confidence: 99%

Anticancer effects of tanshinone I in human non-small cell lung cancer

Lee¹,

Sher

Chen

et al. 2008

Molecular Cancer Therapeutics

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116

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Tanshinones are the major bioactive compounds of Salvia miltiorrhiza Bunge (Danshen) roots, which are used in many therapeutic remedies in Chinese traditional medicine. We investigated the anticancer effects of tanshinones on the highly invasive human lung adenocarcinoma cell line, CL1-5. Tanshinone I significantly inhibited migration, invasion, and gelatinase activity in macrophage-conditioned medium-stimulated CL1-5 cells in vitro and also reduced the tumorigenesis and metastasis in CL1-5-bearing severe combined immunodeficient mice. Unlike tanshinone IIA, which induces cell apoptosis, tanshinone I did not have direct cytotoxicity. Real-time quantitative PCR, luciferase reporter assay, and electrophoretic mobility shift assay revealed that tanshinone I reduces the transcriptional activity of interleukin-8, the angiogenic factor involved in cancer metastasis, by attenuating the DNA-binding activity of activator protein-1 and nuclear factor-KB in conditioned medium-stimulated CL1-5 cells. Microarray and pathway analysis of tumorrelated genes identified the differentially expressed genes responding to tanshinone I, which may be associated with the Ras-mitogen-activated protein kinase and Rac1 signaling pathways. These results suggest that tanshinone I exhibits anticancer effects both in vitro and in vivo and that these effects are mediated at least partly through the interleukin-8, Ras-mitogen-activated protein kinase, and Rac1 signaling pathways. Although tanshinone I has a remarkable anticancer action, its potential anticoagulant effect should be noted and evaluated.

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“…It is now believed that the anti-inflammatory effect of theophylline is caused by its ability to inhibit the responses of these proinflammatory cells Barnes, 2003). For example, it has been shown that the therapeutic concentration of theophylline (55-110 M) can significantly inhibit the expression and release of TNF-␣, IL-8, IL-5, and IL-13 from human blood monocytes/alveolar macrophages in vitro (Spatafora et al, 1994;Kimura et al, 2003;Yao et al, 2005). In vivo, the drug has been shown to significantly reduce the number of eosinophils and T cells present in the bronchoalveolar lavage fluid of asthmatics (Kidney et al, 1995;.…”

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confidence: 99%

Interactions between Theophylline and Salbutamol on Cytokine Release in Human Monocytes

Ezeamuzie

Shihab²

2010

J Pharmacol Exp Ther

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The combination of ␤ 2 -adrenoceptor agonists (␤ 2 -agonists) with inhaled steroids has become the standard treatment for mild to moderate asthma. Theophylline has also been combined successfully with inhaled steroids. However, the possible interaction between theophylline and ␤ 2 -agonists, with regard to their anti-inflammatory effects, has not been clarified. The aim of this study was to investigate the in vitro interaction between theophylline and salbutamol on cytokine generation from human monocytes and compare it with a similar interaction between dexamethasone and salbutamol. Purified monocytes from normal donors were pretreated with the drugs (alone or in combination) and stimulated with lipopolysaccharide for 24 h. Released tumor necrosis factor-␣ (TNF-␣) and interleukin-6 (IL-6), and their corresponding mRNA expressions, were determined and analyzed. Salbutamol (Ն 0.1 M) significantly inhibited the release of TNF-␣, but also significantly enhanced that of IL-6. In contrast, theophylline (50 M) and dexamethasone (0.1 M) strongly inhibited the generation of both cytokines. It is noteworthy that when the drugs were used in combination the effects of theophylline and salbutamol were additive in inhibiting TNF-␣ release, but theophylline blocked the IL-6-enhancing effect of salbutamol. A similar effect was seen when dexamethasone was combined with salbutamol. These results show that ␤ 2 -agonists have opposing effects on the generation of TNF-␣ and IL-6, but that when they were combined with clinically relevant concentrations of theophylline, theophylline, like dexamethasone, was capable of augmenting the anti-inflammatory effects of the ␤ 2 -agonists while at the same time preventing their proinflammatory effect. Thus, theophylline may have a potentially useful steroid-sparing effect.

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Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation

Cited by 21 publications

References 41 publications

Serum amyloid A (SAA) in induced sputum of asthmatics: A new look to an old marker

Serum amyloid A (SAA) in induced sputum of asthmatics: A new look to an old marker

Anticancer effects of tanshinone I in human non-small cell lung cancer

Interactions between Theophylline and Salbutamol on Cytokine Release in Human Monocytes

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