Global distribution of mating types shows limited opportunities for mating across populations of fungi causing boxwood blight disease

Malapi‐Wight, Martha; Veltri, Daniel; Gehesquière, Bjorn; Heungens, Kurt; Rivera, Yazmín; Salgado‐Salazar, Catalina; Crouch, Jo Anne

doi:10.1016/j.fgb.2019.103246

Cited by 16 publications

(15 citation statements)

References 95 publications

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“…This was consequently recognised as a new mating type gene and is designated here as MAT1-2-12 . This gene was previously designated as MAT1-2-2 by Malapi-Wight et al (2019) .…”

Section: Resultsmentioning

confidence: 99%

“…All additional available genome sequences for Calonectria spp. published to date ( Malapi-Wight et al 2016a , b , 2019 , Ye et al 2017 , LeBlanc et al 2019 ) were also screened for inclusion in this study of the mating type locus. These included three genome sequences of Ca.…”

Section: Methodsmentioning

confidence: 99%

“…Mating strategies have consequently served as important criteria in the taxonomy of Calonectria ( Schoch et al 1999 , Lombard et al 2010a ). Similarly, using genome sequences and PCR amplification of MAT genes, populations of Calonectria species have been defined based on their mating type ( Malapi-Wight et al 2014 , 2019 ). For example, Malapi-Wight et al (2019) showed in a collection from four continents, that all isolates of Ca.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, using genome sequences and PCR amplification of MAT genes, populations of Calonectria species have been defined based on their mating type ( Malapi-Wight et al 2014 , 2019 ). For example, Malapi-Wight et al (2019) showed in a collection from four continents, that all isolates of Ca. henricotiae were MAT1-1 whereas all isolates of Ca.…”

Section: Introductionmentioning

confidence: 99%

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Mating genes in Calonectria and evidence for a heterothallic ancestral state

Wingfield

et al. 2020

persoonia

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The genus Calonectria includes many important plant pathogens with a wide global distribution. In order to better understand the reproductive biology of these fungi, we characterised the structure of the mating type locus and flanking genes using the genome sequences for seven Calonectria species. Primers to amplify the mating type genes in other species were also developed. PCR amplification of the mating type genes and multi-gene phylogenetic analyses were used to investigate the mating strategies and evolution of mating type in a collection of 70 Calonectria species residing in 10 Calonectria species complexes. Results showed that the organisation of the MAT locus and flanking genes is conserved. In heterothallic species, a novel MAT gene, MAT1-2-12 was identified in the MAT1-2 idiomorph; the MAT1-1 idiomorph, in most cases, contained the MAT1-1-3 gene. Neither MAT1-1-3 nor MAT1-2-12 was found in homothallic Calonectria (Ca.) hongkongensis, Ca. lateralis, Ca. pseudoturangicola and Ca. turangicola. Four different homothallic MAT locus gene arrangements were observed. Ancestral state reconstruction analysis provided evidence that the homothallic state was basal in Calonectria and this evolved from a heterothallic ancestor.

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“…This was consequently recognised as a new mating type gene and is designated here as MAT1-2-12 . This gene was previously designated as MAT1-2-2 by Malapi-Wight et al (2019) .…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mating genes in Calonectria and evidence for a heterothallic ancestral state

Wingfield

et al. 2020

persoonia

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“…2019 , LeBlanc et al. 2019 , Malapi-Wight et al. 2019 ), emphasizing a need for population biology studies on these fungi in the future.…”

Section: Discussionmentioning

confidence: 99%

Reconsideration of species boundaries and proposed DNA barcodes for Calonectria

Liu

Wingfield

et al. 2020

Studies in Mycology

123

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Calonectria represents a genus of phytopathogenic ascomycetous fungi with a worldwide distribution. In recent years, there has been an increase in the number of taxonomic studies on these fungi. Currently, there are 169 described species of Calonectria based on comparisons of DNA sequence data, combined with morphological characteristics. However, for some of these species, the sequence data utilised at the time of their description were relatively limited. This has justified an urgent need to reconsider the species boundaries for Calonectria based on robust genus-wide phylogenetic analyses. In this study, we utilised 240 available isolates including the ex-types of 128 Calonectria species, and re-sequenced eight gene regions ( act , cmdA , his3 , ITS, LSU, rpb2, tef1 and tub2 ) for them. Sequences for 44 Calonectria species, for which cultures could not be obtained, were downloaded from GenBank. DNA sequence data of all the 169 Calonectria species were then used to determine their phylogenetic relationships. As a consequence, 51 species were reduced to synonymy, two new species were identified, and the name Ca. lauri was validated. This resulted in the acceptance of 120 clearly defined Calonectria spp. The overall data revealed that the genus includes 11 species complexes, distributed across the Prolate and Sphaero-Naviculate Groups known to divide Calonectria . The results also made it possible to develop a robust set of DNA barcodes for Calonectria spp. To accomplish this goal, we evaluated the outcomes of each of the eight candidate DNA barcodes for the genus, as well as for each of the 11 species complexes. No single gene region provided a clear identity for all Calonectria species. Sequences of the tef1 and tub2 genes were the most reliable markers; those for the cmdA , his3 , rpb2 and act gene regions also provided a relatively effective resolution for Calonectria spp., while the ITS and LSU failed to produce useful barcodes for species discrimination. At the species complex level, results showed that the most informative barcodes were inconsistent, but that a combination of six candidate barcodes ( tef1 , tub2 , cmdA , his3 , rpb2 and act ) provided stable and reliable resolution for all 11 species complexes. A six-gene combined phylogeny resolved all 120 Calonectria species, and revealed that tef1 , ...

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