Global detection and identification of components from crude and processed traditional Chinese medicine by liquid chromatography connected with hybrid ion trap and time‐of‐flight‐mass spectrometry

Abstract

We herein present a chemical profiling method to efficiently process the information acquired by ultra fast liquid chromatography (UFLC)-electrospray ionization source in combination with hybrid ion trap and high-resolution time-of-flight mass spectrometry (UFLC-(ESI)-IT-TOF/MS), facilitating the structural determination of serial components contained in crude or processed traditional Chinese medicine (TCM). Under the optimized UFLC and IT-TOF-MS(n) conditions, over 39 compounds were separated and detected in crude or processed Fructus corni within 25 min. The components were identified by comparing the mass spectra and retention time with reference compounds, or tentatively assigned by elucidating low-energy collision-induced dissociation (CID) fragment ions and matching empirical molecular formula with that of the published compounds. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used to elucidate the processed mechanism of F. corni, which is regularly affected by the processing conditions. This study provides a novel approach and methodology to identify the complicated components from various complex mixtures such as crude TCM, processed TCM, and biological samples. It can be used as a valid analytical method for further understanding the processing mechanism of TCM, along with the intrinsic quality control of TCM and its processed product.

Investigation of the Effect of Rice Wine on the Metabolites of the Main Components of Herbal Medicine in Rat Urine by Ultrahigh-Performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry: A Case Study onCornus officinalis

“…et Zucc (Cornaceae) is a herbal medicine widely used in TCM for medical, food sanitation, and cosmetic purposes [10]. Clinically, it is used both in its crude herbal form and as a processed product [11].…”

Qualitative analysis of major constituents from Xue Fu Zhu Yu Decoction using ultra high performance liquid chromatography with hybrid ion trap time‐of‐flight mass spectrometry

“…Sixteen reference standards of gallic acid (1), chlorogenic acid (4), amygdalin (5), hydroxysafflor yellow A (6), albiflorin (7), ferulic acid (11), liquiritin (15), narirutin (17), naringin (20), hesperidin (21), neohesperidin (22), liquiritigenin (24), isoliquiritigenin (27), glycyrrhizic acid (32), ligustilide (33), and glycyrrhetinic acid (34) were purchased from Chengdu Mann Stewart Biological Technology (Chengdu, China) or Shanghai Source Biological Technology (Shanghai, China). The purity of all standards was above 98%, as revealed by HPLC/UV analysis.…”

“…23,[25][26][27], compounds 10, 13, 16, and 25 were tentatively identified.Liquiritin(15), narirutin(17), naringin(20), hesperidin(21), and neohesperidin(22) contained flavanones aglycone. Liquiritin (15) (C 21 H 22 O 9 ) presented an [M+H] + ion with an accurate mass at m/z 419.1378 and an [M-H] − ion with an accurate mass at m/z 417.1190.…”

Comparative pharmacokinetics of the main compounds of Shanzhuyu extract after oral administration in normal and chronic kidney disease rats

“…Cornus officinalis, a species of dogwood, has been used for thousands of years as an important folk medicine, and it is also considered as one of the 25 vegetable drugs that are most frequently applied in PR China, Japan and Republic of Korea (Cao et al, 2011;Han et al, 2014;Ma et al, 2014). Cornus officinalis exhibits a number of biological activities, including immunological regulation, reducing blood glucose, antishock, antiarrhythmia and antibiosis (Lee et al, 2011;Park et al, 2011).…”