Jon M. Steichen, Ganesh H. Iyer, Sheng Li, S. Adrian Saldanha, Michael S. Deal, Virgil L. Woods, Susan S. Taylor
Abstract:Phosphorylation of the activation loop is one of the most common mechanisms for regulating protein kinase activity. The catalytic subunit of cAMP-dependent protein kinase autophosphorylates Thr197 in the activation loop when expressed in Escherichia coli. Although mutation of Arg194 to Ala prevents autophosphorylation, phosphorylation of Thr197 can still be achieved by a heterologous protein kinase, phosphoinositide-dependent protein kinase (PDK1), in vitro. In this study, we examined the structural and functi…
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