Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in�vitro

Xu, Tanzhen; Shen, X. Y.; Sun, Lixian; Chen, Yali; Zhang, Bi‐Qiong; Huang, Dake; Li, Weizu

doi:10.3892/ijmm.2018.4005

Cited by 36 publications

(41 citation statements)

References 46 publications

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“… 24 , 25 Chronic glucocorticoid exposure significantly activated NLRP1 inflammasome in hippocampal neurons, thus to increase neuron-inflammation. 28 Ginsenoside Rg1 protected against neuronal degeneration by inhibiting NLRP1 inflammasome both in H 2 O 2 -treated hippocampal neurons in vitro 9 and in dexamethasone-treated mice. 29 NLRP1 inflammasome activation was proved to result in pyroptosis, a caspase-1-dependent inflammatory form of cell death that is distinct from apoptosis or other cell death mechanisms.…”

Section: Discussionmentioning

confidence: 99%

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Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease

Zhang¹,

Guan²,

Tan³

et al. 2021

NDT

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Background It was indicated that nucleotide-binding oligomerisation domain‑like receptor protein 1 (NLRP1) inﬂammasome-mediated pyroptosis is involveg in the progression of Alzheimer’s disease (AD). This study was designed to explore the effect of Bushen Huoxue Acupuncture on cognitive defect and NLRP1 inflammasome-mediated pyroptosis in AD mouse. Methods Senescence-accelerated mouse prone 8 (SAMP8) mice were used as a model of AD. Bushen Huoxue Acupuncture was performed in four acupoints: “Baihui acupoint” (GV20), “Shenshu acupoint” (BL23), “Xuehai acupoint” (SP10), and “Geshu acupoint” (BL17). Morris water maze test was performed to evaluate the cognitive function of the mouse. The levels of Aβ 1-40 , Aβ 1-42 , IL-1β, and IL-18 were examined by ELISA assay. Neuronal apoptosis and damage in hippocampal tissues were measured using TUNEL and Nissl staining, respectively. The expression of NLRP1, ASC, cleaved caspase-1, IL-1β, and IL-18 was examined using Western blot. Results Bushen Huoxue Acupuncture improved the learning and memory deficits of AD mouse. Meanwhile, Bushen Huoxue Acupuncture decreased the production of Aβ in hippocampal tissues of SAMP8 mice and attenuated the neuronal apoptosis and damage. Furthermore, Bushen Huoxue Acupuncture inhibited NLRP1 inﬂammasome activation in SAMP8 mice. Conclusion Bushen Huoxue Acupuncture could notably attenuate the cognitive defect of mouse AD model and inhibit NLRP1 inflammasome-mediated pyroptosis.

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Section: Discussionmentioning

confidence: 99%

“… 8 Xu et al reported that Ginsenoside Rg1 protect against H 2 O 2 -induced neuronal damage by inhibiting the activation of NLRP1 inflammasome in hippocampal neurons in vitro. 9 These findings suggested that the strategies targeting NLRP1 inflammasome activation might be useful in the therapy of AD.…”

Section: Introductionmentioning

confidence: 97%

Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease

Zhang¹,

Guan²,

Tan³

et al. 2021

NDT

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“…Ginsenoside Rg1 (Rg1) is a bioactive component in ginseng, which has been used to delay senescence since ancient times. Our previous studies suggested that Rg1 could ameliorate H 2 O 2 -induced hippocampal neuron damage in vitro [12]. The APP/PS1 transgenic mouse is the most commonly used AD model animal, which exhibits obvious elevation of Aβ deposition and behavioral abnormalities.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous research indicated that the expressions of NOX2 and its subunits of Rac1, p47phox and p22phox were signi cantly increased in long-term cultured hippocampal neurons [17]. Meanwhile, treatment with Rg1 signi cantly decreased the expressions of NOX2 and p47phox in H 2 O 2 -induced hippocampal neurons [12]. Therefore, we speculated that Rg1 treatment might inhibit NOX2-mediated neuronal oxidative stress and neuroin ammation in APP/PS1 AD mice.…”

mentioning

confidence: 92%

“…It has been reported to be a potential neuroprotective agent in several animal models of neurological diseases and cognitive impairments [10,11]. Our previous study demonstrated that Rg1 was able to alleviate H 2 O 2 -induced neuronal damage by inhibiting NADPH oxidase 2 (NOX2) and nucleotide-binding oligomerization domain (NOD)-like receptor protein 1 (NLRP1) in ammasome activation in hippocampal neurons in vitro [12]. These data suggested that Rg1 treatment might delay the occurrence and development of AD through its antioxidant and anti-in ammatory effects.…”

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confidence: 99%

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Ginsenoside Rg1 alleviates Aβ deposition and neuronal damage by inhibiting NLRP1 inflammasome activation in APP/PS1 mice

Zhang¹,

Su²,

Sun³

et al. 2020

Preprint

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Background: Oxidative stress and neuroinflammation play important roles in the whole pathogenesis of Alzheimer's disease (AD). NADPH oxidase 2 (NOX2) is an important enzyme that is responsible for ROS generation in many neurodegenerative diseases. The nucleotide-binding oligomerization domain (NOD)-like receptor protein 1 (NLRP1) inflammasome is responsible for the formation of pro-inflammatory molecules in neurons. However, it is still unclear whether inhibition of NOX2 and NLRP1 inflammasome decreases amyloid-beta (Aβ) generation and deposition in APP/PS1 mice. Ginsenoside Rg1 (Rg1) is an active component in ginseng, and maybe a potential agent for neurodegenerative diseases. In this study, we investigated the effects and mechanisms of Rg1 treatment on cognitive performance, neuronal damage, Aβ deposition and NOX2-NLRP1 inflammasome activation in APP/PS1 mice.Methods: WT and APP/PS1 mice were used in the experiment from 6 months (M) old to 9M old, and 6M APP/PS1 mice were used as pre-treatment controls. The open field test (OFT) and Morris water maze (MWM) were used to detect behavioral change and cognitive function. The H&E and Nissl staining were used to assess neuronal damage. We further examined PSD95 expression, Aβ generation and deposition, Tau and p-Tau expression, NOX2-mediated ROS generation and NLRP1 inflammasome activation by using immunofluorescence, western blot analysis, and real time q-PCR.Results: Rg1 treatment for 12 weeks alleviated learning and memory impairments and neuronal damage, decreased p-Tau level, APP expression and Aβ deposition in APP/PS1 mice. Meanwhile, Rg1 treatment significantly decreased the levels of ROS and IL-1β, and reduced the expressions of NOX2 and NLRP1 inflammasome in the brain cortex and hippocampus in APP/PS1 mice. Furthermore, apocynin (a NOX inhibitor) and NLRP1-siRNA treatment also alleviated cognitive impairments, neuronal damage and Aβ deposition, and reduced the expression of NLRP1 inflammasome in brain cortex and hippocampus in APP/PS1 mice.Conclusions: Rg1 treatment could alleviate learning and memory impairment, neuronal damage, and reduce Aβ generation and deposition by inhibiting NLRP1 inflammasome activation in APP/PS1 mice.

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The roles of natural triterpenoid saponins against Alzheimer's disease

Zhou

et al. 2023

Phytotherapy Research

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The aging of the world population and increasing stress levels in life are the major cause of the increased incidence of neurological disorders. Alzheimer's disease (AD) creates a huge burden on the lives and health of individuals and has become a big concern for society. Triterpenoid saponins (TS), representative natural product components, have a wide range of pharmacological bioactivities such as anti‐inflammation, antioxidation, antiapoptosis, hormone‐like, and gut microbiota regulation. Notably, some natural TS exhibited promising neuroprotective activity that can intervene in AD progress, especially in the early stage. Recently, studies have indicated that TS play a pronounced positive role in the prevention and treatment of AD. This review discusses the recent research on the neuroprotection of TS and proceeds to detail the action mechanisms of TS against AD, hoping to provide a reference for drug development for anti‐AD.

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Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in�vitro

Cited by 36 publications

References 46 publications

Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease

Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease

Ginsenoside Rg1 alleviates Aβ deposition and neuronal damage by inhibiting NLRP1 inflammasome activation in APP/PS1 mice

The roles of natural triterpenoid saponins against Alzheimer's disease

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