Ginkgo biloba repair spinal cord ischemia reperfusion injury in rats by regulating Ferroptosis signaling pathway

Abstract

Ferroptosis is a hot topic in recent years. As a new type of cell death, its main signs are iron overload and lipid peroxidation.Spinal cord ischemia reperfusion injury (SCIRI) is often accompanied by reactive oxygen-induced oxidative stress, and the spinal cord is rich in polyunsaturated fatty acids, so it is very easy to undergo lipid peroxidation under the attack of oxygen free radicals after ischemia reperfusion injury, and eventually lead to degeneration and necrosis of nerve cells.Therefore, inhibition of reactive oxygen species accumulation is essential to reduce nerve cell death after SCIRI.Our previous studies have shown that Ginkgo biloba (GB) can remove oxygen free radicals produced during ischemia reperfusion in the spinal cord of rats, but the specific mechanism of action is unclear.In this study, we first obtained the genes of the SCIRI group and model group through bioinformatics analysis, and then intersects the genes related to ferroptosis to verify the most specific genes.After subsequent experiments, we found that Ginkgo biloba extract (GBE) can significantly increase glutathione peroxidase 4 (GPX4),solute carrier family7 member11 (SLC7A11) and augmenter of liver regeneration (ALR), decrease the expression of 4-hydroxy-2-nonenal (4-HNE) and transferrin receptor 1 (TfR1), alleviating nerve injury after SCIRI in rats.