Gingival crevicular fluid level of soluble triggering recep-tor expressed on myeloid cells-1(strem-1) in periodontal health and disease: a case-control study

Abstract

Background: The Triggering Receptor Expressed On Myeloid Cells-1(TREM-1) is a cell-surface receptor of the immunoglobulin superfamily and found to be involved in the amplification of the inflammatory response to various microbial infections, including periodontal diseases. Objectives: The present study was designed to examine gingivalcrevicular fluid(GCF) levels of soluble TREM-1 (sTREM-1) levels in periodontal health and disease as well as evaluate the effect of scaling and/or root planing on the same. Methods: Based on gingival index, probing pocket depth, clinical attachment level, and radiologic parameters (bone loss), 45 subjects were initially divided into three groups-Group, I (Periodontally healthy), Group II (Gingivitis) and Group III (Chronic Periodontitis).From each of the subjects, GCF sample was collected at baseline and scaling and/or root planing was instituted in group II and group III patients. GCF samples were subsequently collected at eight-week interval. Levels of sTREM-1 in collected GCF samples were estimated using enzyme-linked immunosorbent assay. Results: The lowest GCF levels of sTREM-1 were found in periodontal health (69.50±1.8pg/ml) followed by gingivitis (257.17±79pg/ml) and chronic periodontitis (3658.14±55pg/ml) in increasing order, suggesting that levels of sTREM-1 in crevicular fluid increased with the severity of periodontal disease. sTREM-1 levels decreased significantly from baseline to the end of 8 weeks following non-surgical periodontal therapy. Conclusion: Increased GCF levels of sTREM-1 from periodontal health to disease strengthen its association with periodontal status.

Evaluation of “soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), interleukin-1β, and matrix metalloproteinase-8” as a short panel of salivary biomarkers in patients with and without stage III/IV periodontitis and type 2 diabetes mellitus

“…In our study, all the three evaluated biomarkers ("sTREM-1, IL-1β and MMP-8") were found to be elevated in the periodontitis groups compared to the healthy groups. In the Bostanci et al study, 22 they had observed a 3.3-5.6 fold raised salivary sTREM-1 levels in advanced periodontitis cases and similarly, Belibasakis et al in 2014 23 and Surabhigigras et al in 2017 24 reported increased sTREM-1 levels in disease sites and all forms of periodontitis. The results of our study also supports these findings.…”