GhLIP1, a lipoic acid synthase gene, negatively regulates leaf senescence in cotton

GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance

“…To assess the phylogenetic relationships among GhBEE3-Like and its homologous proteins, the BLAST program in NCBI ( ) was applied to search all homologous genes, except BEE3 gene. The phylogenetic tree was generated in MEGA software (version 7.0) by adopting the neighbor-joining (NJ) algorithm, and the parameter setting was adopted from the previous study ( Chen et al., 2021 ).…”

“…To analyze the functions of GhBEE3-Like gene under drought stress. VIGS technology was applied to produce TRV2 (control) and TRV2:GhBEE3-Like ( GhBEE3-Like gene knock-down) cottons according to previous studies ( Pang et al., 2013 ; Chen et al., 2021 ). In the VIGS technology system, GhPDS was used as a marker gene to evaluate the presence of albino phenotype in TRV2:GhPDS cotton after knock-down.…”

“…To examine whether GhBEE3-Like is highly expressed in Arabidopsis , the leaves of Col-0 and transgenic lines were collected for total RNA extraction and cDNA synthesis. PCR was carried out in a heated-lid thermal cycler (Applied Biosystems, USA) as previously described ( Chen et al., 2021 ). The primers of the internal reference AtUBQ10 (At4g05320) are listed in Supplementary Table 1 .…”

“…To analyze the tissue expression patterns of GhBEE3-Like gene and whether GhBEE3-Like can respond to drought stress or BR treatment, the expression levels of GhBEE3-Like and stress-related genes were detected using the Applied Biosystems QuantStudio 6 Flex system according to a previous report ( Chen et al., 2021 ). GhHIS3 gene was employed as an internal reference.…”