GhCYS2 governs the tolerance against cadmium stress by regulating cell viability and photosynthesis in cotton

Meng, Yuan; Cui, Yupeng; Peng, Fanjia; Guo, Lixue; Cui, Ruifeng; Xu, Nan; Huang, Hui; Han, Mingge; Fan, Yapeng; Zhang, Menghao; Sun, Yupin; Wang, Lidong; Yang, Zhining; Liu, Mengyue; Chen, Wenhua; Ni, Kesong; Wang, Delong; Zhao, Lanjie; Lu, Xuke; Chen, Xiugui; Wang, Junjuan; Wang, Shuai; Ye, Wuwei

doi:10.1016/j.ecoenv.2023.115386

Cited by 1 publication

(1 citation statement)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For determination of proline (Pro) and malondialdehyde (MDA) contents, the leaves of control and NaCl-treated cotton seedlings were collected at each time point and immediately frozen in liquid nitrogen and stored at −80°C to determine the content of Pro and MDA. The content of Pro was determined using the test kit from Nanjing Jiancheng Bioengineering Institute, the content of MDA was determined using the test kit from Beijing Solarbio Science & Technology Co., Ltd. ( Liu et al., 2022 ; Meng et al., 2023 ).…”

Section: Experimental Materials and Methodsmentioning

confidence: 99%

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

Chen,

Cui,

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton.

show abstract

Section: Experimental Materials and Methodsmentioning

confidence: 99%