GhCDNC and GhCYP706B1 genes mediate gossypol biosynthesis in upland cotton

Maryam, Hira; Ali, Zulfiqar; Saddique, Muhammad Abu Bakar; Nawaz, Fahim

doi:10.1007/s11033-022-07355-8

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…Twenty μL final volume of PCR reactions containing 12.8 μL ddH 2 O, 1 μL cDNA (20 ng/μL) as reported by Maryam et al [ 14 ], 0.4 μL of each primer (10 μM) (list of primers are given in Table 3 ), 2 μL rTaq buffer (10×), 1.6 μL MgCl 2 (25 mM), 1.6 μL dNTPs (10 mM), and 0.2 μL rTaq DNA polymerase (5 U/μL) (Takara Bio Inc.; Shiga, Japan). PCR was performed in a thermo-cycler (T100 Thermal Cycler, Bio-Rad Laboratories, Inc., Hercules, CA, USA) set with an initial denaturation temperature of 94 °C for 3 min, 35 cycles of denaturation at 94 °C for 30 s, annealing at 50–60 °C for 30 s and extension at 72 °C for 60 s/kb of amplicon followed by a final extension at 72 °C for 3 min/kb of the amplicon.…”

Section: Methodsmentioning

confidence: 99%

“…PCR was performed in a thermo-cycler (T100 Thermal Cycler, Bio-Rad Laboratories, Inc., Hercules, CA, USA) set with an initial denaturation temperature of 94 °C for 3 min, 35 cycles of denaturation at 94 °C for 30 s, annealing at 50–60 °C for 30 s and extension at 72 °C for 60 s/kb of amplicon followed by a final extension at 72 °C for 3 min/kb of the amplicon. Resolve amplicons on 1% agarose gel [ 14 , 15 ]. The band intensity was identified on ImageJ software ( accessed on 6 December 2021) [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…These dwarfing genes are well known to reduce cell size in coleoptiles, culms and leaves of wheat. As compared to standard height genotypes ( Rht - B1a and Rht-D1a ) of wheat, GA-insensitive wheat is poor in the case of leaf area development [ 14 ]. The alternative height-reducing genes (GA-sensitive) Rht8 and Rht13 shorten plant height and enhanced grain yield [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…As compared to standard height genotypes ( Rht - B1a and Rht-D1a ) of wheat, GA-insensitive wheat is poor in the case of leaf area development [ 14 ]. The alternative height-reducing genes (GA-sensitive) Rht8 and Rht13 shorten plant height and enhanced grain yield [ 14 ]. The GA-sensitive ( Rht13 ) is the most important dwarfing gene that controls the plant height of wheat without influencing the seedling growth, grain number and grain yield [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Effects of GA-Responsive Dwarfing Gene Rht13 on Plant Height, Peduncle Length, Internodal Length and Grain Yield of Wheat under Drought Stress

Khalid

Ali

Tahir

et al. 2023

Genes

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Reduction in plant height is generally associated with an increase in lodging resistance, drought tolerance and grain yield of wheat worldwide. Historically, a significant increase in grain yield was observed through the introduction of semi-dwarf wheat varieties utilizing the gibberellic acid-insensitive Rht genes (Rht1 or Rht2). The gibberellic acid sensitive (GA-sensitive) reduced height (Rht) genes are available that are alternatives to gibberellic acid insensitive (GA-insensitive) Rht genes, having a neutral effect on coleoptile length seedling vigor suggesting their potential in using alone or in combination with GA-insensitive Rht genes to improve grain yield and drought tolerance in wheat. This study was conducted to evaluate parents and F1 crosses under drought stress. The crossing was done using line × tester mating design, comprising eight lines and five testers having different GA-sensitive and GA-insensitive Rht genes. Parents and F1 crosses were sown in the field under RCBD with three replications in normal and drought stress. Data were recorded for morpho-physiological traits. The mean comparison showed significant differences among parents and hybrids for most of the studies’ traits. The general combining ability showed that line 1 is the good general combiner for days to heading, lodging (%), plant height, peduncle length, internodal length and days to maturity under normal conditions while L5 was the good general cobiner for chlorophyll contents and stomatal conductance both under normal and drought stress. The spcaicfic combing ability estimases showed that the cross L1 × T1 was best for days to heading, lodging (%), plant height and internodal length both under normal and drought stress. F1 hybrids showed a significant reduction in plant height (18–25%), peduncle length (20–28%) and increased grain yield (15–18%) under drought stress. Expression analysis showed upregulation of Rht13 at the middle part of the peduncle internode under drought stress. From the expression analysis, five crosses were selected, and their segregating population was raised and space-plated. Rht13 genes reduced plant height (−30 to −45%), peduncle length (−30 to −53%), peduncle internode length (−28% to −48%), increased spike length (+20% to +50%), number of grains per spike (+17 to +26%) and grain yield per plant (+29% to +50%) compared to Rht1 gene. These results suggested the possibility of using the GA-sensitive Rht13 gene for the development of high-yielding and drought-tolerant wheat varieties.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Effects of GA-Responsive Dwarfing Gene Rht13 on Plant Height, Peduncle Length, Internodal Length and Grain Yield of Wheat under Drought Stress

Khalid

Ali

Tahir

et al. 2023

Genes

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The nutritional and industrial significance of cottonseeds and genetic techniques in gossypol detoxification

Ninkuu,

Liu,

Zhou

et al. 2023

Plants People Planet

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Societal Impact StatementGossypol and its derivatives represent a class of toxic and immunosuppressive compounds that are naturally synthesized in cottonseed. These compounds pose several health hazards to humans and animals, such as heart and lung damage, breathing difficulties, and death in swine. In poultry, gossypol reduces egg production and slows growth. Studies have also shown that gossypol can indirectly harm humans and animals through the food chain. Although several physical and chemical approaches are adopted to reduce gossypol levels in cottonseed before food and feed processing, these techniques are expensive. Therefore, genetically regulating gossypol production in cotton could provide a cheaper alternative.SummaryCotton (Gossypium spp.), the most important fiber crop, is cultivated in over a hundred countries to provide raw materials for the growing textile industry. The seed obtained after delinting cotton is a rich source of protein with a vast potential for oil and feed production. Cottonseed oil production is estimated at 5.08 million metric tons and is expected to generate over 6.56 billion United States Dollars in revenue by 2029. The cake from defatted cottonseed is used as animal feed and food supplements. However, the contamination of gossypol in cottonseed limits its use. Gossypol ingestion impairs weight gain and causes anorexia, respiratory distress, and death under extreme exposure. This review highlights the significance of cottonseed oil and meal; the pharmacological uses and impact of gossypol; the chemistry, toxicity, and bioactivity of gossypol; and the physical and chemical methods used in gossypol removal during feed and food supplement processing. In addition, the biosynthetic pathway of gossypol and attempts to genetically transform some key regulators of this pathway to produce glandless cottonseed or reduce the gossypol levels in the seed are discussed.

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