Getting the big beast to work—Systems biotechnology of Bacillus megaterium for novel high-value proteins

Korneli, Claudia; David, Florian; Biedendieck, Rebekka; Jahn, Dieter; Wittmann, Christoph

doi:10.1016/j.jbiotec.2012.06.018

Cited by 47 publications

(33 citation statements)

References 82 publications

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“…For example the Gram-positive soil bacterium Bacillus megaterium, has been used for many decades in industrial biotechnology. B. megaterium has a high secretion capacity directly into the surrounding medium as it is lacking an outer cell membrane [8]. Many reports of the usefulness of bacteria strain in the plant cultivation can be found in the literature.…”

Section: Introductionmentioning

confidence: 99%

Production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium

et al. 2015

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Abstract:In this paper, the production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium is presented. The biofertilizers used in this study contain phosphorus compounds that are in available form to plants as well as components of growth medium. The solubilization was performed under two conditions; with chlorides and with sulphates instead of chlorides. Three biofertilizer forms are proposed in relation to the doses of bones applied in the solubilization process (4, 10 or 20 g L -1 ). The solubilization degree varied according to the bacterial medium formulation and the bones doses. The replacement of chlorides with sulphates yielded a lower growth rate, and resulted, in a lower solubilization. The specific growth rate of the cells of B. megaterium in a sulphate medium was lower than compared with the specific growth rate of cell culture in a medium of chlorides of about 22.4, 39 and 14%, for 4, 10 and 20 g L -1 of bones concentration, respectively. In the stationary phase, the solubilization factor (SF) was higher (61.7%) for the solubilization process conducted in a medium with chlorides − C bone 4 g L -1 , compared with the solubilization process conducted in the medium of sulphates (52.7%).

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Section: Introductionmentioning

confidence: 99%

Production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium

et al. 2015

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“…Vorteile sind das Fehlen alkalischer Proteasen und eine stabile Plasmidreplikation. Als Bodenbakterium ist es ein guter Proteinsekretierer und kann eine breite Auswahl an Kohlenstoffquellen, u. a. auch Alt-Glycerin oder Melasse [2,3], nutzen, was für nachhaltige Kultivierungsprozesse von Bedeutung ist.…”

Section: Bacillus Megaterium Als Expressionswirtunclassified

“…1: Bacillus megaterium-Vektoren. Ausgangsshuttlevektor (A) und Variationen des Vektors bezüglich Promotor (Xylose-, Saccharoseinduzierbar; Phagen-Promotoren T7, K1E, SP6) (B) [5,8,10], Affinitätstag (C) [7,10], Signalsequenz (SP) (D) [5,10], Systeme zur Sekretion und Reinigung (E) [3,5] …”

Section: Bacillus-expressionssystemeunclassified

Bacillus megaterium — ein Produktionssystem für rekombinante Proteine

et al. 2014

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During the past years plasmid systems for recombinant protein production using Bacillus megaterium were developed. These systems are based on multicopy plasmids with functional elements for cloning in Escherichia coli and induced gene expression in B. megaterium. Different promoters, signals for protein secretion and affinity tags for purification arranged in a high variety of combinations are available. By using these systems, recombinant proteins can be produced and secreted in the grams per litre scale.

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“…In urge of improvement in salinity tolerance worldwide, use of biological agents has been reported from many years (Glick et al, 2007;Yang et al, 2008;Zahir et al, 2008;Adesemoye et al, 2009;Bhattacharyya and Jha, 2012). These microbes termed as plant growth promoting rhizobacteria (PGPR), regulate hormonal and nutritional balance, solubilize nutrients, produce plant growth regulators and induce resistance in plants (Korneli et al, 2012;Nadeem et al, 2014). Plant growth promoting rhizobacteria is a varied group of colonizing bacteria, which When grown with plants cause growth stimulation and direct indirect promotions (Vessey, 2003;Banchio et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

PGPR Potentially Improve Growth of Tomato Plants in Salt-Stressed Environment

Zameer

Zahid

Tabassum

et al. 2016

Turkish JAF Sci.Tech.

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Plant growth promoting rhizobacteria are colonized bacterial species that has the capability to improve plant growth by certain direct and indirect means. Environmental factors including both biotic and abiotic stresses are among the major constraints to crop production. In the current study, the effectiveness of microbial inoculation (Bacillus megaterium) for enhancing growth of tomato plants under salt stress conditions has been investigated. Significant improvement in shoot length, root length, leaf surface area, number of leaves, total weight of the shoot and root was observed in tomato plants inoculated with zm7 strain post 15 and 30 days of its application. Zm3, Zm4 and Zm6 strains improved the morphological parameters as compared to the control. Chlorophyll content a, chlorophyll content b, anthocyanin and carotenoid content was increased in tomato plants subjected to Zm7, Zm6 and Zm4 strains. Stress responsive genes; metallothionein and glutothion gene were found highly expressed in Zm7 treated tomato plants as compared to control, untreated plants. Significant correlation of anthocyanin was reported for carotenoids, chlorophyll-b, shoot weight and total weight of seedling while carotenoids were significantly correlated with leaf surface area, root length, chlorophyll-b and anthocyanin. Overall, Zm7 strain proved best for improvement in salt stressed plant’s morphological parameters and biochemical parameters as compared to control, untreated plants.

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Getting the big beast to work—Systems biotechnology of Bacillus megaterium for novel high-value proteins

Cited by 47 publications

References 82 publications

Production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium

Production of phosphate biofertilizers from bones by phosphate-solubilizing bacteria Bacillus megaterium

Bacillus megaterium — ein Produktionssystem für rekombinante Proteine

PGPR Potentially Improve Growth of Tomato Plants in Salt-Stressed Environment

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