Genotyping and drug resistance patterns of <i>Mycobacterium tuberculosis</i> strains in five provinces of China

Guo, Yuxin; Y, Liu; Sm, Wang; Cy, Li; Gl, Jiang; Gl, Shi; Cx, Song

doi:10.5588/ijtld.10.0403

Cited by 18 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is similar to the two previous large-scale studies conducted in China [9], [10]. Dong et al analyzed 2,346 M. tuberculosis isolates from 13 provinces and showed that Beijing family isolates were prevalent all over the country (74.08%) [9], while Guo et al reported that 77.8% of 158 strains isolated in five provinces belonged to the Beijing genotype [10]. However, this rate was much lower (62.2%) in a recent study of 4017 isolates from the National Drug Resistance Base-Line Surveillance of Tuberculosis [11].…”

Section: Discussionsupporting

confidence: 92%

“…Our study found that the Beijing family strains were prevalent in the total collection (79.5%). This result is similar to the two previous large-scale studies conducted in China [9], [10]. Dong et al analyzed 2,346 M. tuberculosis isolates from 13 provinces and showed that Beijing family isolates were prevalent all over the country (74.08%) [9], while Guo et al reported that 77.8% of 158 strains isolated in five provinces belonged to the Beijing genotype [10].…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China

Jiao

Liu²,

Han³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

BackgroundTuberculosis (TB) is still a big threat to human health, especially in children. However, an isolation of Mycobacterium tuberculosis culture from pediatric cases remains a challenge. In order to provide some scientific basis for children TB control, we investigated the genotyping and drug resistance characteristics of M. tuberculosis isolates from pediatric cases in China.Methodology/Principal FindingsIn this study, a total of 440 strains including 90 from children (<15 years), 159 from adolescents (15–18 years) and 191 from adults (>18 years) isolated in 25 provinces across China were subjected to spoligotyping and drug susceptibility testing. As a result, Beijing family strains were shown to remain predominant in China (85.6%, 81.1% and 75.4% in three above groups, respectively), especially among new children cases (91.0% vs. 69.6% in previously treated cases, P = 0.03). The prevalence of the Beijing genotype isolates was higher in northern and central China in the total collection (85.1% in northern and 83.9% in central vs. 61.6% in southern China, P<0.001) and a similar trend was seen in all three age groups (P = 0.708, <0.001 and 0.025, respectively). In adolescents, the frequencies of isoniazid (INH)-resistant and ethambutol (EMB)-resistant isolates were significantly higher among Beijing strains compared to non-Beijing genotype strains (P = 0.028 for INH and P = 0.027 for EMB). Furthermore, strong association was observed between resistance to rifampicine (RIF), streptomycin (STR) and multidrug resistance (MDR) among Beijing compared to non-Beijing strains in previously treated cases of children (P = 0.01, 0.01 and 0.025, respectively).Conclusion/SignificanceBeijing family was more prevalent in northern and central China compared to southern China and these strains were predominant in all age groups. The genetic diversity of M. tuberculosis isolates from children was similar to that found in adolescents and adults. Beijing genotype was associated with RIF, STR and MDR resistance in previously treated children.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 86%

A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China

Jiao

Liu²,

Han³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Particular M. tuberculosis genotypes have been found to be related to the risk of transmission. Previous studies [6], [16]–[18] have described the distribution of various genotypes of TB strains from many areas in China. However, a detailed analysis of the population structure of TB strains from the metropolitan area of Beijing, one of the largest cities in the world, with a high prevalence of TB, has not been conducted.…”

Section: Discussionmentioning

confidence: 99%

Genotypic Diversity Analysis of Mycobacterium tuberculosis Strains Collected from Beijing in 2009, Using Spoligotyping and VNTR Typing

et al. 2014

View full text Add to dashboard Cite

BackgroundTuberculosis (TB) is a serious problem in China. While there have been some studies on the nationwide genotyping of Mycobacterium tuberculosis (M. tuberculosis), there has been little detailed research in Beijing, the capital of China, which has a huge population. Here, M. tuberculosis clinical strains collected in Beijing during 2009 were genotyped by classical methods.Methodology/Principal FindingsOur aim was to analyze the genetic diversity of M. tuberculosis strains within the Beijing metropolitan area. We characterized these strains using two standard methods, spoligotyping (n = 1585) and variable number of tandem repeat (VNTR) typing (n = 1053). We found that the most prominent genotype was Beijing family genotype. Other genotypes included the MANU, T and H families etc. Spoligotyping resulted in 137 type patterns, included 101 unclustered strains and 1484 strains clustered into 36 clusters. In VNTR typing analysis, we selected 12-locus (QUB-11b, MIRU10, Mtub21, MIRU 23, MIRU39, MIRU16, MIRU40, MIRU31, Mtub24, Mtub04, MIRU20, and QUB-4156c) and named it 12-locus (BJ) VNTR. VNTR resulted in 869 type patterns, included 796 unclustered strains and 257 strains clustered into 73 clusters. It has almost equal discriminatory power to the 24-locus VNTR.Conclusions/SignificanceOur study provides a detailed characterization of the genotypic diversity of M. tuberculosis in Beijing. Combining spoligotyping and VNTR typing to study the genotyping of M. tuberculosis gave superior results than when these techniques were used separately. Our results indicated that Beijing family strains were still the most prevalent M. tuberculosis in Beijing. Moreover, VNTR typing analyzing of M. tuberculosis strains in Beijing was successfully accomplished using 12-locus (BJ) VNTR. This method used for strains genotyping from the Beijing metropolitan area was comparable. This study will not only provide TB researchers with valuable information for related studies, but also provides guidance for the prevention and control of TB in Beijing.

show abstract

“…These results are in accordance with data from previous studies using MIRU-VNTR typing for genotyping of strain collections comprising a higher proportion of Beijing strains. It has already been shown that 12-loci MIRU-VNTR typing was not sufficient to discriminate Beijing family strains (6,9). Similarly, Hanekom and colleagues reported that even 15-and 24-loci MIRU-VNTR typing has a reduced discriminatory power compared to IS6110 typing for strains of the Beijing lineage in Cape Town, South Africa, and that the inclusion of more variable loci might be necessary to reach the same level as that with IS6110 RFLP typing (7).…”

Section: Discussionmentioning

confidence: 99%

“…In comparative populationbased evaluations, we and others found a similar if not slightly better performance of 15-or 24-loci MIRU-VNTR typing compared to IS6110 RFLP typing for discrimination of clinical isolates and detection of transmission chains in TB patient populations (13). Few subsequent studies compared MIRU-VNTR typing with the "gold standard" IS6110 RFLP typing, because the latter is rather laborious and more difficult to perform (6,7,15). The majority of the studies confirmed the initial finding that 24-loci MIRU typing can be used for MTBC genotyping with a similar performance as IS6110 typing, but some studies suggested a lower discriminatory power of 24-loci MIRU-VNTR typing in settings where Beijing strains are dominant (7,15).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Mycobacterium tuberculosis Typing Methods in a 4-Year Study in Schleswig-Holstein, Northern Germany

et al. 2011

View full text Add to dashboard Cite

In order to evaluate the discriminatory power of different methods for genotyping of Mycobacterium tuberculosis complex (MTBC) isolates, we compared the performance of (i) IS6110 DNA fingerprint typing, (ii) spoligotyping, and (iii) 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing in a long-term study on the epidemiology of tuberculosis (TB) in Schleswig-Holstein, the northernmost federal state of Germany. In total, we analyzed 277 MTBC isolates collected from patients between the years 2006 and 2010. The collection comprised a broad spectrum of 13 different genotypes, among which strains of the Haarlem genotype (31%) were most prominent, followed by strains belonging to the Delhi and Beijing lineages (7% and 6%, respectively). On the basis of IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping analyses, 211 isolates had unique patterns (76%) and 66 isolates (24%) were in 20 clusters. MIRU-VNTR combined with spoligotyping analyses revealed 202 isolates with unique patterns (73%) and 75 isolates in 18 clusters (27%). Overall, there was 93.1% concordance between the typing results obtained; 198 strains were identified as unique, and 60 isolates were clustered by both typing combinations (including all 31 isolates with confirmed epidemiological links). Of the remaining 19 isolates with discrepant results, 15 were falsely clustered by MIRU-VNTR (six Beijing genotype strains) and four were clustered by IS6110 RFLP (low IS6110 copy number) only. In conclusion, in the study population investigated, a minority of isolates, especially of the Beijing genotype, clustered by standard 24-loci MIRU-VNTR and without an obvious epidemiological link may require second-line typing by IS6110 RFLP or hypervariable MIRU-VNTR loci.

show abstract

Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains in five provinces of China

Abstract: The M. tuberculosis Beijing genotype varies in prevalence in different regions of China and is solely associated with RMP resistance.

Cited by 18 publications

References 0 publications

A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China

A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China

Genotypic Diversity Analysis of Mycobacterium tuberculosis Strains Collected from Beijing in 2009, Using Spoligotyping and VNTR Typing

Evaluation of Mycobacterium tuberculosis Typing Methods in a 4-Year Study in Schleswig-Holstein, Northern Germany

Contact Info

Product

Resources

About