Genotypic versus phenotypic methods in the detection of Listeria monocytogenes prosthetic joint infection

Žaloudíková, Barbora; Kelbl, Martin; Paša, Libor; Freiberger, Tomáš

doi:10.1099/jmm.0.006106-0

Cited by 6 publications

(14 citation statements)

References 17 publications

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“…A remarkable feature of prosthetic joint infection with L. monocytogenes is the long period between implantation of the prosthetic device and manifestation of the infection. The late occurrence of the infection in prosthetic joints suggests a transient bacteremia and hematogenous route of infection and most likely is not associated with the surgical operation itself [1][2][3][4]7,12,[14][15][16]18,20,21]. As in the present patient, the disease expresses as severe joint pain and fever.…”

Section: Discussionmentioning

confidence: 97%

“…Isolation of bacteria from multiple samples increases the likelihood of diagnosis [2,12]. Co-infection with S. aureus has been reported [22].…”

Section: Discussionmentioning

confidence: 99%

“…Nieman et al, were the first to report in 1980, on L. monogytogenes arthritis [11]. Meningitis and sepsis in neonatal infants, bacteremia, endocarditis, prosthetic valve endocarditis, and endophthalmitis are the infections reported most frequently; osteomyelitis, septic arthritis, and prosthetic joint infections are rare [1][2][3][4][5]7,[12][13][14][15][16][17][18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…In this setting, molecular methods of pathogen detection such as broad-range polymerase chain reaction and sequencing are useful when a rare or unexpected micro-organism is present or the sample is collected during antibiotic treatment [2]. This distinction is clinically relevant because standard treatment for S. aureus with a penicillinase-resistant penicillin is inadequate for Listeria [23].…”

mentioning

confidence: 98%

“…They have been described in prosthetic valves, stent grafts, and prosthetic joints as a result of the high affinity of the microorganism for foreign bodies [1,2]. Prosthetic joint infections usually occur in patients with malignant disease, diabetes mellitus, chronic kidney disease, or liver disease, or in elderly or immunocompromised patients [3][4][5][6].…”

mentioning

confidence: 99%

See 4 more Smart Citations

Late Hip Arthroplasty Infection Caused byListeria monocytogenesin a Non-Immunocompromised Patient

Mavrogenis

Savvidou

Vlasis

et al. 2011

Surgical Infections

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Predisposing conditions and co-morbidities usually are required for L. monocytogenes infection. Listeria is fastidious and is not a recognized laboratory isolate or hospital contaminant; routine cultures therefore may be negative. In approximately one-half of the reported cases, the prosthetic joint infections were treated successfully by two-stage revision surgery plus long-term antibiotic therapy. In most cases, ampicillin and gentamicin are the first choice. Cephalosporins are rarely effective.

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Section: Discussionmentioning

confidence: 97%

“…Isolation of bacteria from multiple samples increases the likelihood of diagnosis [2,12]. Co-infection with S. aureus has been reported [22].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 3 more Smart Citations

Late Hip Arthroplasty Infection Caused byListeria monocytogenesin a Non-Immunocompromised Patient

Mavrogenis

Savvidou

Vlasis

et al. 2011

Surgical Infections

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Unusual Listeria monocytogenes hematogenous infection in total knee replacement treated with one-stage revision surgery

et al. 2019

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Septic arthritis due to Listeria monocytogenes (LM) is extremely rare and most infections due to this organism are seen in immunocompromised patients. We describe a patient without immunological compromise, with a late total knee arthroplasty infection caused by LM treated with one-stage revision surgery. She had an elevated erythrocyte sedimentation rate (79 mm/h) and C-reactive protein (13 mg/dL). Aspiration of the knee joint yielded purulent fluid; cultures showed LM. The patient was given 6 weeks of intravenous ampicillin, followed by trimethoprim/sulfamethoxazole, and finally amoxicillin orally for 7 months. Two years after revision surgery, radiographs showed no evidence of implant loosening. This is a single case and although one-stage approach seemed to have worked, it should not be recommended on the basis of a single report.

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Genomic analysis of a rare recurrent Listeria monocytogenes prosthetic joint infection indicates a protected niche within biofilm on prosthetic materials

Hutchins

Sayavedra

Díaz

et al. 2021

Sci Rep

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Listeria monocytogenes is a rare cause of prosthetic joint infections (PJI). In this study, we describe a case of recurrent L. monocytogenes infections, 39 months apart, following debridement and retention of a prosthetic hip. Despite numerous studies reporting persistent L. monocytogenes in human infections, the genomic and phenotypic changes that clinically relevant strains undergo in the host are poorly understood. Improved knowledge of how PJI occurs is needed to improve the management of prosthetic infections. We used a combination of long- and short-read sequencing to identify any potential genomic differences between two L. monocytogenes isolates that occurred over 39-month incubation in the host. The isolates, QI0054 and QI0055, showed three single nucleotide polymorphisms and three insertions or deletions, suggesting that the recurrent infection was caused by the same strain. To identify potential differences in the capacity for persistence of these isolates, their biofilm-forming ability and potential to colonize prosthesis-relevant materials was investigated both in microtitre plates and on prosthetic material titanium, stainless steel 316 and ultra-high molecular weight polyethylene. Whilst the L. monocytogenes isolate from the most recent infection (QI0055) was able to form higher biofilm in microtitre plates, this did not lead to an increase in biomass on prosthetic joint materials compared to the initial isolate (QI0054). Both clinical isolates were able to form significantly more biofilm on the two metal prosthetic materials than on the ultra-high molecular weight polyethylene, in contrast to reference strain Scott A. Transcriptomics revealed 41 genes overexpressed in biofilm state and 643 in planktonic state. Moreover, genes with mutations were actively expressed in both isolates. We conclude the isolates are derived from the same strain and hypothesize that L. monocytogenes formed biofilm on the prosthetic joint materials, with minimal exposure to stresses, which permitted their survival and growth.

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Genotypic versus phenotypic methods in the detection of Listeria monocytogenes prosthetic joint infection

Cited by 6 publications

References 17 publications

Late Hip Arthroplasty Infection Caused byListeria monocytogenesin a Non-Immunocompromised Patient

Late Hip Arthroplasty Infection Caused byListeria monocytogenesin a Non-Immunocompromised Patient

Unusual Listeria monocytogenes hematogenous infection in total knee replacement treated with one-stage revision surgery

Genomic analysis of a rare recurrent Listeria monocytogenes prosthetic joint infection indicates a protected niche within biofilm on prosthetic materials

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