Genotypic variation in salt tolerance of Ulmus pumila plants obtained by shoot micropropagation

Mu, Dianzani; Zwiazek, Janusz J.; Li, Zhouqi; Zhang, Wenqing

doi:10.1007/s11738-016-2189-4

Cited by 5 publications

(9 citation statements)

References 69 publications

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“…We found that high accumulation of sugars and more activities of SOD enzyme in leaf tissue contribute to the osmotic adjustment and ROS scavenging system under salinity treatment; the sugar content and SOD activity play key roles in U. pumila's tolerance to salt stress, and are promising indicators for U. pumila species ex vitro selections. The ex vitro selection results align with the previous in vitro studies [37] and is promising for the MVF development.…”

supporting

confidence: 83%

“…Urban forestry also adopted varietal deployment (e.g., vegetative reproduction) for horticultural application. In broad-leaf trees such as elms, MVF is promising as the result of the tissue culture and somatic embryogenesis technology for the landscape-scale deployment of elite varieties that are tolerant to abiotic stresses such as salinity [37]. MVF also enables the tree variety production from ex vitro selection for urban forest development.…”

Section: Introductionmentioning

confidence: 99%

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Developing a salinity tolerance indicator for tree varieties at challenging sites and urban forests based on inferences of physiological responses: an example of Ulmus pumila

Ding

2021

Trees

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Elevated salinity is one of the major environmental limitation factors of plant growth and development and salinity stress compromises the production and survival of plantation and urban forests and agricultural crops in the arid, semi-arid, and intertidal zones. Ulmus pumila, a salt-indigenous tree species in Asia and is widely deployed in salt-affected areas in China, and U.pumila is promising for multi-varietal forestry in plantation and urban forests. The comprehensive mechanism of the intraspecific salt tolerance is still not clear yet. Here, we investigated the physiological responses of the salinity stress based on the antioxidant enzyme activities, osmotic adjustments, and gas exchange among salt-tolerant U. pumila genotypes for 100 days under five different NaCl levels (0%, 0.3%, 0.5%, 0.7%, and 0.9% w/v) with natural surroundings and rain shade at age-2. Salt stress decreased height (HR), ground diameter (DR), and dry weight (biomass) were significantly different among genotypes. HR and performance indices were positively correlated with photosynthesis rate (Pn), apparent mesophyll conductance (AMC), and chlorophyll (CHLL) with (r= 0.7 -0.8 ***), but were negatively related to the free proline, sugar, and protein accumulation (r=-0.5 ~ -0.7***). We found that high accumulation of sugars and more activities of SOD enzyme in leaf tissue contribute to the osmotic adjustment and ROS scavenging system under salinity treatment; the sugar content and SOD activity play key roles in U. pumila's tolerance to salt stress, and are promising indicators for U. pumila species ex vitro selections. The ex vitro selection results align with the previous in vitro studies [37] and is promising for the MVF development.

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supporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Developing a salinity tolerance indicator for tree varieties at challenging sites and urban forests based on inferences of physiological responses: an example of Ulmus pumila

Ding

2021

Trees

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“…Humans use the stem and root parts of U. pumila for the treatment of various ailments such as edema, mastitis, gastric cancer, and inflammation in the traditional system of medicine [9,10]. Not surprisingly, a number of bioactive natural products have been identified from extracts of U. pumila [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Elm (Ulmus pumila) Fruit to Identify Phytonutrients Associated Genes and Pathways

Zhang¹,

Li²,

Wang³

et al. 2019

Forests

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Plant fruit is an important source of natural active phytonutrients that are profitable for human health. Elm (Ulmus pumila) fruit is considered as natural plant food in China that is rich in nutrients. In the present study, high-throughput RNA sequencing was performed in U. pumila edible fruits and leaves and 11,386 unigenes were filtered as dysregulated genes in fruit samples, including 5231 up- and 6155 downregulated genes. Hundreds of pathways were predicted to participate in seed development and phytonutrient biosynthesis in U. pumila by GO, MapMan, and KEGG enrichment analysis, including “seed maturation”, “glycine, serine, and threonine metabolism” and “phenylpropanoid biosynthesis”. ABA-mediated glucose response-related ethylene-activated signaling pathway (e.g., ABI4) were supposed to associate with elm fruit development; unsaturated fatty acids pathway (e.g., ACX2 and SAD) were predicted to participate in determination of fatty acid composition in elm fruit; flavonoid and coumarins biosynthesis (e.g., CYP98A3 and CCoAOMT1) were demonstrated to correlate with the bioactivity of elm fruits in human cancer and inflammation resistance. To provide more information about fruit developmental status, the qRT-PCR analysis for key genes of “phenylpropanoid biosynthesis” and “alpha-Linolenic acid metabolism” were conducted in samples of young fruits, ripe fruit, old fruit, and leaves. Two biosynthetic pathways for unsaturated fatty acid and Jasmonic acid (JA) were deduced to be involved in fruit development in U. pumila and the phenylpropanoid glycoside, syringin, was speculated to accumulate in the early development stages of elm fruit. Our transcriptome data supports molecular clues for seed development and biologically active substances in elm fruits.

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“…China has 100 million hm 2 of salinized land suitable for the cultivation of saline-tolerant bioenergy tree species. Ulmus pumila is highly adaptable to adverse conditions, with disease resistance, cold tolerance and drought hardiness, and high tolerance to salt and barren soil [16][17][18][19][20]. It has been reported to be able to tolerate 100-150 mM NaCl (0.3-0.5%), and its growth is not affected at these concentrations [21].…”

Section: Introductionmentioning

confidence: 99%

Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance in Ulmus pumila

Chen

Liu

Zhang

et al. 2021

IJMS

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Ulmus pumila L. is an excellent afforestation and biofuel tree that produces high-quality wood, rich in starch. In addition, U. pumila is highly adaptable to adverse environmental conditions, which is conducive to its utilization for vegetating saline soils. However, little is known about the physiological responses and transcriptional regulatory network of U. pumila under salt stress. In this study, we exposed five main cultivars in saline–alkali land (Upu2, 5, 8, 11, and 12) to NaCl stress. Of the five cultivars assessed, Upu11 exhibited the highest salt resistance. Growth and biomass accumulation in Upu11 were promoted under low salt concentrations (<150 mM). However, after 3 months of continuous treatment with 150 mM NaCl, growth was inhibited, and photosynthesis declined. A transcriptome analysis conducted after 3 months of treatment detected 7009 differentially expressed unigenes (DEGs). The gene annotation indicated that these DEGs were mainly related to photosynthesis and carbon metabolism. Furthermore, PHOTOSYNTHETIC ELECTRON TRANSFERH (UpPETH), an important electron transporter in the photosynthetic electron transport chain, and UpWAXY, a key gene controlling amylose synthesis in the starch synthesis pathway, were identified as hub genes in the gene coexpression network. We identified 25 and 62 unigenes that may interact with PETH and WAXY, respectively. Overexpression of UpPETH and UpWAXY significantly increased the survival rates, net photosynthetic rates, biomass, and starch content of transgenic Arabidopsis plants under salt stress. Our findings clarify the physiological and transcriptional regulators that promote or inhibit growth under environmental stress. The identification of salt-responsive hub genes directly responsible for photosynthesis and starch synthesis or metabolism will provide targets for future genetic improvements.

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Genotypic variation in salt tolerance of Ulmus pumila plants obtained by shoot micropropagation

Cited by 5 publications

References 69 publications

Developing a salinity tolerance indicator for tree varieties at challenging sites and urban forests based on inferences of physiological responses: an example of Ulmus pumila

Developing a salinity tolerance indicator for tree varieties at challenging sites and urban forests based on inferences of physiological responses: an example of Ulmus pumila

Transcriptome Analysis of Elm (Ulmus pumila) Fruit to Identify Phytonutrients Associated Genes and Pathways

Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance in Ulmus pumila

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