Genotypic Diversity of Culturable Vibrio Species Associated with the Culture of Oysters and Clams in Galicia and Screening of Their Pathogenic Potential

Guisande, José Antonio; Lago, Estela Pérez; Prado, Susana; Nieto, T. P.; Seguin, Rosa Farto

doi:10.2983/0730-8000(2008)27[801:gdocvs]2.0.co;2

Cited by 13 publications

(24 citation statements)

References 31 publications

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“…For bacteria, experimental infections are also often used to explore pathogenicity (Gay et al, 2004;Goarant et al, 2006b), coupled with phenotypic and/or molecular characterization of virulence factors. Such experiments are used in epidemiological studies to assess the dynamics of infectious diseases (Goarant et al, 2006a;Guisande et al, 2008;Saulnier et al, 2000).…”

Section: Potential and Limitations Of Bath Challenges Vs Injectionmentioning

confidence: 99%

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

Decker

Normand

Saulnier

et al. 2011

Journal of Invertebrate Pathology

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Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohistochemical analyses showed the affinity of Vibrio for gonadic tissues, highlighting the importance of sexual maturity for vibriosis infection processes in oysters. Mortality rate results showed poor repeatability between tanks, however, in this bath challenge. We then tested a standardized and repeatable injection protocol using two different doses of the same combination of two Vibrio species on related diploid and triploid oysters at four different times over a year. Statistical analyses of mortality kinetics over a 6-day period after injection revealed that active gametogenesis periods correspond to higher susceptibility to vibriosis and that there is a significant interaction of this seasonal effect with ploidy. However, no significant advantage of triploidy was observed. Triploid oysters even showed lower survival than diploid counterparts in winter. Results are discussed in relation to differing energy allocation patterns between diploid and triploid Pacific oysters.

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Section: Potential and Limitations Of Bath Challenges Vs Injectionmentioning

confidence: 99%

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

Decker

Normand

Saulnier

et al. 2011

Journal of Invertebrate Pathology

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“…TH-K8, which was most closely related to V. kanaloae by 16S rRNA gene sequences, was pathogenic to Strongylocentrotus intermedius larvae. The pathogenicity of V. kanaloae for mollusks has previously been reported (Guisande et al 2008), but we suggest that Vibrio sp. TH-K8 is pathogenic for echinoderms.…”

Section: Discussionmentioning

confidence: 82%

Involvement of the cell-specific pigment genes pks and sult in bacterial defense response of sea urchins Strongylocentrotus intermedius

Kiselev

Ageenko²,

Kurilenko³

2013

Dis. Aquat. Org.

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Bacterial infections are one of the most important problems in mass aquaculture, causing the loss of millions of juvenile organisms. We isolated 22 bacterial strains from the cavity fluid of the sea urchin Strongylocentrotus pallidus and used phylogenetic analysis based on 16S rRNA gene sequences to separate the bacterial strains into 9 genera (Aliivibrio, Bizionia, Colwellia, Olleya, Paenibacillus, Photobacterium, Pseudoalteromonas, Shewanella, and Vibrio). Incubating Strongylocentrotus intermedius larvae with a strain from each of the 9 bacterial genera, we investigated the viability of the larvae, the amount of pigment cells, and the level of polyketide synthase (pks) and sulfotransferase (sult) gene expression. Results of the assay on sea urchin development showed that all bacterial strains, except Pseudoalteromonas and Bizionia, suppressed sea urchin development (resulting in retardation of the embryos' development with cellular disorders) and reduced cell viability. We found that pks expression in the sea urchin larvae after incubation with the bacteria of 9 tested genera was significantly increased, while the sult expression was increased only after the treatment with Pseudoalteromonas and Shewanella. Shikimic acid, which is known to activate the biosynthesis of naphthoquinone pigments, increased the tolerance of the sea urchin embryos to the bacteria. In conclusion, we show that the cell-specific pigment genes pks and sult are involved in the bacterial defense response of sea urchins.

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“…The findings of Garnier et al (2007) showed the evidence for the involvement of V. splendidus group in summer mortality of C. gigas in the gonadal maturation phase. Two strains of V. splendidus caused the death of the clam, Venerupis rhomboids (Pennant, 1777) by bath challenge (Guisande et al 2008). Larvae and juveniles of oyster (C. gigas) (Sugumar et al 1998, Waechter et al 2002 and carpet shell clams (Ruditapes decussatus) (G omez-Le on et al 2005), their mortality has been associated with V. splendidus biovar II.…”

Section: Discussionmentioning

confidence: 97%

“…The predominant species, Vibrio tasmaniensis was associated with the culture of oysters and clams (Guisande et al 2008). Lago et al (2009) demonstrated that five strains of V. tasmaniensis from healthy reproductive oyster (Guisande et al 2008) colonized the tissues of Venerupis rhomboids and induced disease in clam. This species has also been reported as the cause of disease skin ulceration in sea cucumber (Deng et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…For massive mortalities of different bivalves both in the hatchery (for larval and postlarval stages) and in the worldwide (for juveniles and adults) field, Vibrio has been reported as the primary pathogen (Nicolas et al 1996, Paillard et al 2004, Torkildsen et al 2005, Sandlund et al 2006, Guisande et al 2008, Kesarcodi-Watson et al 2009, Beaz-Hidalgo et al 2010a, 2010b, Teng et al 2012, Genard et al 2013, Liu et al 2013, Prado et al 2014. In China, frequent outbreaks of vibriosis have caused high mortality of cultured Yesso scallop, resulting in huge-economic losses (Teng et al 2012, Liu et al 2013; however, information about the identity of pathogenic bacteria causing mortality in the Yesso scallop larvae is limited.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Vibrio Infections Associated with Yesso Scallop (Patinopecten yessoensis) Larval Culture

Liu¹,

Sun²,

Li³

et al. 2015

Journal of Shellfish Research

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Genotypic Diversity of Culturable Vibrio Species Associated with the Culture of Oysters and Clams in Galicia and Screening of Their Pathogenic Potential

Cited by 13 publications

References 31 publications

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

Involvement of the cell-specific pigment genes pks and sult in bacterial defense response of sea urchins Strongylocentrotus intermedius

Vibrio Infections Associated with Yesso Scallop (Patinopecten yessoensis) Larval Culture

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