Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of <i>Escherichia Coli</i> Strains from Piglets in Korea

Lee, Su-In; Rayamahji, Nabin; Lee, Won Jung; Bin, Seung; Shin, Min Kyung; Roh, Yu Mi; Yoo, Han Sang

doi:10.1177/104063870902100413

Cited by 15 publications

(8 citation statements)

References 18 publications

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“…In this study, all VTEC isolated were multidrug-resistant, in which high resistance rates were observed for Amp, Aml, Cip, Enr, Tet, Sxt, Chl and Nal (Figure 1). This is similar to recent reports from Thailand and Korea [15,26]. This suggests that the swine farms in those countries may have used the similar antimicrobials for prophylactics as the swine farms in Malaysia.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia

Tan

Ooi

et al. 2013

BMC Vet Res

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BackgroundPostweaning diarrhea caused by pathogenic Escherichia coli, in particular verotoxigenic E. coli (VTEC), has caused significant economic losses in the pig farming industry worldwide. However, there is limited information on VTEC in Malaysia. The objective of this study was to characterize pathogenic E. coli isolated from post-weaning piglets and growers with respect to their antibiograms, carriage of extended-spectrum beta-lactamases, pathotypes, production of hemolysins and fimbrial adhesins, serotypes, and genotypes.ResultsPCR detection of virulence factors associated with different E. coli pathotypes (ETEC, EPEC, EHEC, and VTEC) revealed that VTEC was the only pathotype identified from six swine farms located at north-western Peninsular Malaysia. A low prevalence rate of VTEC was found among the swine samples (n = 7/345) and all 7 VTEC isolates were multidrug resistant. Five of these isolates from different hosts raised in the same pen were likely to be of the same clone as they shared identical sero-pathotypes (O139:H1, VT2e/α-hly/F18), resistance profiles and DNA fingerprinting profiles. Two other serotypes, O130: H26 (n = 1) and O168: H21 (n = 1) carrying virulence factors were also identified. O168: H21 is possibly a new serotype as this has not been previously reported.ConclusionsThe occurrence of VTEC with infrequently encountered serotypes that are multidrug resistant and harbouring virulence factors may be of public health concern. The detection of possible clones in this study also showed that the combination of different typing tools including phenotyping and genotyping methods is useful for molecular epidemiologic surveillance and studies.

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Section: Discussionsupporting

confidence: 89%

Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia

Tan

Ooi

et al. 2013

BMC Vet Res

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“…A study performed in Korea showed how E. coli strains isolated from diarrhoeic pigs were multi-resistant (resistant to more than 4 antibiotics) with high levels of resistance to several antibiotics: gentamicin (77%), trimethoprim-sulphametoxazole (75.7%), amoxicillin (75.7%), ampicillin (73%) and enrofloxacin (64.9%) [ 57 ].…”

Section: Introductionmentioning

confidence: 99%

Swine enteric colibacillosis: diagnosis, therapy and antimicrobial resistance

2017

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Intestinal infection with enterotoxigenic Escherichia coli (ETEC) is an important disease in swine resulting in significant economic losses. Knowledge about the epidemiology, the diagnostic approach and methods of control are of fundamental importance to tackle the disease. The ETEC causing neonatal colibacillosis mostly carry the fimbriae F4 (k88), F5 (k99), F6 (987P) or F41, while the ETEC of post-weaning diarrhoea carry the fimbriae F4 (k88) and F18. These fimbriae adhere to specific receptors on porcine intestinal brush border epithelial cells (enterocytes), starting the process of enteric infection. After this colonization, the bacteria produce one or more enterotoxins inducing diarrhoea, such as the heat stable toxin a (STa), the heat stable toxin b (STb), and the heat labile toxin (LT). A role in the pathogenesis of the disease was demonstrated for these toxins. The diagnosis of enteric colibacillosis is based on the isolation and quantification of the pathogenic E.coli coupled with the demonstration by PCR of the genes encoding for virulence factors (fimbriae and toxins). The diagnostic approach to enteric colibacillosis must consider the differential diagnosis and the potential different causes that can be involved in the outbreak.Among the different methods of control of colibacillosis, the use of antimicrobials is widely practiced and antibiotics are used in two main ways: as prophylactic or metaphylactic treatment to prevent disease and for therapeutic purposes to treat diseased pigs.An accurate diagnosis of enteric colibacillosis needs an appropriate sampling for the isolation and quantification of the ETEC responsible for the outbreak by using semi-quantitative bacteriology. Definitive diagnosis is based on the presence of characteristic lesions and results of bacteriology along with confirmation of appropriate virulence factors to identify the isolated E.coli. It is important to confirm the diagnosis and to perform antimicrobial sensitivity tests because antimicrobial sensitivity varies greatly among E. coli isolates. Growing concern on the increase of antimicrobial resistance force a more rational use of antibiotics and this can be achieved through a correct understanding of the issues related to antibiotic therapy and to the use of antibiotics by both practitioners and farmers.

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“…In our previous studies of antimicrobial resistance in Enterobacteriaceae isolated from farm animals, we identified some important antimicrobial resistance genes associated with transferable plasmids of similar sizes. In addition to antibiotic resistance genes, plasmids may also bear important toxin genes that could be maintained and disseminated to a wide range of microbes, especially members of the Enterobacteriaceae, from farm animals that share common environmental niches (9,10,13,15).…”

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Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

Rayamajhi

Bin

Shin

et al. 2011

Appl Environ Microbiol

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In this study, we focused on determining the distribution and prevalence of major plasmid replicons in ␤-lactam-resistant Escherichia fergusonii and Enterobacteriaceae of animal and human origin. A high degree of plasmid variability and multiple plasmid replicons were observed among the isolates. The IncF and IncI1 replicons were the most prevalent in E. fergusonii and Salmonella enterica serovar Indiana isolated from swine and poultry in South Korea, respectively. The presence of broad-host-range plasmid replicons such as IncN, IncA/C, IncHI1, and IncHI2 that are associated with important virulence genes and toxins as well as antimicrobial resistance determinants indicates that E. fergusonii has the potential to become an important pig pathogen and possible emerging opportunistic zoonotic pathogen.

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Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

Cited by 15 publications

References 18 publications

Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia

Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia

Swine enteric colibacillosis: diagnosis, therapy and antimicrobial resistance

Plasmid Typing and Resistance Profiling of Escherichia fergusonii and Other Enterobacteriaceae Isolates from South Korean Farm Animals

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