Genotypes and subtypes of Cryptosporidium spp. in diarrheic lambs and goat kids in northern Greece

Papanikolopoulou, Vasiliki; Baroudi, Djamel; Guo, Yaqiong; Wang, Yuanfei; Papadopoulos, Elias; Lafi, Shawkat Q.; El-Tawab, M.M. Abd; Diakou, Αnastasia; Giadinis, Nektarios D.; Feng, Yaoyu; Xiao, Lihua

doi:10.1016/j.parint.2018.04.007

Cited by 32 publications

(29 citation statements)

References 32 publications

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“…Subtypes IIdA18G1 and IIdA22G1 have previously been detected in calves and goat kids in Turkey [22]. In a study conducted in Greece, IIdA16G1 subtype was identified in diarrheic lambs and goat kids [13]. Within the subtype IId family, IIdA16G1, IIdA18G1 and IIdA22G1 subtypes have previously been reported in calves, lambs, and goat kids in Spain [7].…”

Section: Discussionmentioning

confidence: 95%

“…The distribution of Cryptosporidium species in goat kids has been reported to be similar to those of lambs, with the occurrence of mainly C. parvum, C. xiaoi, and C. ubiquitum [7][8][9][10][11][12][13]. However, geographic differences exist in the distribution of Cryptosporidium species in lambs, with C. parvum as the dominant species in European countries, C. ubiquitum in the Americas, C. xiaoi in developing countries, and all three species common in Australia [14].…”

Section: Introductionmentioning

confidence: 98%

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Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Kabir

Ceylan

et al. 2020

Parasitology International

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Background: Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of animals as well as humans. The studies on Cryptosporidium infections of animals in Turkey are mostly rely on microscopic observation. Few data are available regarding the distribution of Cryptosporidium genotypes and subtypes infection. The aim of this study is to analyse the zoonotic potential of Cryptosporidium oocysts shed from young ruminant livestock. Methods: A total of 415 diarrheic fecal specimens from 333 calves, 67 lambs, and 15 goat kids were examined for the presence of Cryptosporidium oocysts by microscopy. Microscopic positive specimens were then analyzed for Cryptosporidium genotypes and subtypes detection by use of nested PCR of the small subunit ribosomal RNA (SSU rRNA) gene and the highly polymorphic 60 kDa glycoprotein (gp60) gene followed by sequence analyses. Results: The results of this study revealed that 25.6% (106 of 415) of the specimens were positive for Cryptosporidium spp. infection by microscopic examination and molecular analysis. We identified 27.4% (91/333), 19.4% (13/67), and 13.4% (2/15) of positivity in calves, lambs and goat kids, respectively. Genotyping of the SSU rRNA indicated that almost all positive specimens were of C. parvum , except for one calf which was of C. bovis. Sequence analysis of the gp60 gene revealed the most common zoonotic subtypes (IIa and IId) of C. parvum. We detected 11 subtypes (IIaA11G2R1,

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Kabir

Ceylan

et al. 2020

Parasitology International

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“…However, it is noteworthy that this prospective cohort study highlighted, when genotyping could be performed, frequent characterization of C. parvum with zoonotic genotypes. Cryptosporidium parvum infections are common in Europe [ 28 , 31 , 37 , 42 ], but not on other continents, where C. xiaoi and C. ubiquitum predominate [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

et al. 2020

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Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C. ubiquitum, C. xiaoi and C. parvum. Due to a lack of relevant information about such infections in France, we investigated the situation on five dairy sheep farms in the Pyrénées-Atlantiques Department in south-western France in December 2017. Individual fecal samples were collected from 79 female lambs (5–17 days old) and their mothers (72 ewes). Oocysts were screened using Heine staining before and after Bailenger concentrations. Cryptosporidium species identification and genotyping were performed using real-time PCR and gp60 gene sequencing. No cases of clinical cryptosporidiosis were observed in the 79 lambs. Microscopically, Cryptosporidium spp. oocysts were observed in only one lamb on one farm (prevalence 1.3%) and one ewe on another farm (prevalence 1.4%). By contrast, Cryptosporidium spp. DNA was detected in 17 ewes (prevalence ranging from 10.5% to 50% depending on the farm) and in 36 lambs (prevalence ranging from 0% to 77.8% depending on the farm). Only zoonotic Cryptosporidium parvum IId and IIa genotypes were identified when genotyping was possible. Cryptosporidium ubiquitum and C. xiaoi were detected on one and three farms, respectively. We conclude that healthy young lambs and their mothers during the peripartum period could be a source of environmental contamination with oocysts.

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“…In the Slovak Republic, only several cases of an infection of humans caused by Cryptosporidium have been reported. The IIdA15G1 subtype, confirmed in Slovakia and only rarely occurring in other countries, induces mild or moderate diarrhoea [29]. The presented case report indicates a potential danger of outbreaks of zoonotic cryptosporidiosis and the need for strict control and hygiene practices during the handling of young animals, especially calves [30,31].…”

Section: Resultsmentioning

confidence: 64%

<i>Cryptosporidium parvum</i> – zoonotic subtype IIdA15G1 in a Slovakian patient

Mravcová¹,

Štrkolcová²,

Mucha³

et al. 2020

Ann Agric Environ Med.

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Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.

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Genotypes and subtypes of Cryptosporidium spp. in diarrheic lambs and goat kids in northern Greece

Cited by 32 publications

References 32 publications

Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Molecular detection of genotypes and subtypes of Cryptosporidium infection in diarrheic calves, lambs, and goat kids from Turkey

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

<i>Cryptosporidium parvum</i> – zoonotic subtype IIdA15G1 in a Slovakian patient

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