Genotype Analysis of Hepatitis C Virus in Brazilian Hemophiliacs and Blood Donors

Martins, Regina Maria Bringel; Barbosa, Adriana P.; Oliveira, Jaqueline Mendes de; Vanderborght, Bart; Yoshida, Clara Fumiko Tachibana

doi:10.1159/000031192

Cited by 2 publications

(3 citation statements)

References 3 publications

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“…The distribution of HCV genotypes in the studied population did not significantly differ from the published data on hemophiliac and non-hemophiliac populations in Latin America, where genotype 1 infection is predominant (9)(10)(11)13,14). Actually, the distribution differs between Brazil and developed countries, where the distribution of HCV genotype among hemophiliacs usually reflects the origin of the blood donors used in the manufacture of pooled factor VIII and IX concentrates, with some of them imported from distant countries (27,28).…”

Section: Discussionmentioning

confidence: 53%

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Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study

Carmo

Oliveira

Guimarães

et al. 2002

Braz J Med Biol Res

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We determined and analyzed risk factors of hepatitis C virus (HCV)-infected Brazilian hemophiliacs according to their virological, clinical and epidemiological characteristics. A cross-sectional and retrospective study of 469 hemophiliacs was carried out at a Brazilian blood center starting in October 1997. The prevalence of HCV infection, HCV genotypes and factors associated with HCV RNA detection was determined. The seroprevalence of anti-HCV antibodies (ELISA-3.0) was 44.6% (209/469). Virological, clinical and epidemiological assessments were completed for 162 positive patients. There were seven (4.3%) anti-HCV seroconversions between October 1992 and October 1997. During the same period, 40.8% of the positive anti-HCV hemophiliacs had abnormal alanine transaminase (ALT) levels. Plasma HCV RNA was detected by nested-RT-PCR in 116 patients (71.6%). RFLP analysis showed the following genotype distribution: HCV-1 in 98 hemophiliacs (84.5%), HCV-3 in ten (8.6%), HCV-4 in three (2.6%), HCV-2 in one (0.9%), and not typeable in four cases (3.4%). Univariate analysis indicated that older age (P = 0.017) and abnormal ALT levels (P = 0.010) were associated with HCV viremia, while the presence of inhibitor antibodies (P = 0.024) and HBsAg (P = 0.007) represented a protective factor against the presence of HCV RNA. These findings may contribute to a better understanding of the relationship between HCV infection and hemophilia.

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Section: Discussionmentioning

confidence: 53%

“…Mixed infection with several genotypes has also been documented in a small minority of patients. The only four studies which assessed HCV genotype distribution in Latin American hemophiliac patients have indicated the predominance of HCV genotype 1 (11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study

Carmo

Oliveira

Guimarães

et al. 2002

Braz J Med Biol Res

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“…Among HIV-1 HCV co-infected subjects, all of them intravenous drug users, HCV subtype 3a predominated and one case was HCV subtype 1a. In selected Brazilian populations, HCV subtype 1 followed by genotypes 3 and 2 have been described [Oliveira et al, 1999;Martins et al, 2000;Campiotto et al, 2005]. The spread of HCV subtypes 3a and 1a among injection drug users co-infected with HIV has been reported by a recent multi-country European study [van Asten et al, 2004].…”

Section: Discussionmentioning

confidence: 99%

Human immunodeficiency virus type 1 and hepatitis C virus Co‐infection and viral subtypes at an HIV testing center in Brazil

Pereira

Stefani

Martelli

et al. 2006

Journal of Medical Virology

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Human immunodeficiency virus (HIV) testing sites have been recognized recently as potential settings for hepatitis C virus (HCV) screening since both viruses share common routes of transmission. HIV and HCV prevalence, predictors, co-infection rates, and viral subtypes were studied in 592 attendants at an anonymous HIV Counseling and Testing Center in central Brazil. Anti-HIV-1 and -HCV antibodies were screened by ELISA, and Western blots were used to confirm HIV infection. Among HIV-seropositive samples, reverse transcriptase-polymerase chain reaction (RT-PCR) and nested-PCR were used to subtype HIV-1 by the Heteroduplex Mobility Analysis (HMA) and HCV by the line probe assay (INNO-LiPA). HIV and HCV seroprevalence was 3.2% (95% CI 2.0-4.9) and 2.5% (95% CI 1.5-4.0), respectively. Intravenous drug use was the risk factor most strongly associated with both HIV and HCV infections, even in a population with few intravenous drug users (n = 6); incarceration was also associated with HCV. HIV/AIDS-positive sexual partner and homosexual/bisexual behaviors were associated independently with HIV-1. The prevalence of HCV infection among HIV-positive persons was 42% (95% CI 20-66), higher than in HIV-negative persons (1.2%; 95% CI 0.5-2.5). HIV-1 subtype B was identified in the env and gag regions of the genome. HCV subtype 3a predominated among co-infected persons and one HCV subtype 1a was detected. Overall, a similar prevalence of HIV and HCV infections and a higher prevalence of HCV among HIV-positive persons were observed. Integrated HIV and HCV screening at HIV testing sites may represent a unique opportunity to provide diagnosis and prevention strategies at a single visit.

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Genotype Analysis of Hepatitis C Virus in Brazilian Hemophiliacs and Blood Donors

Cited by 2 publications

References 3 publications

Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study

Hepatitis C virus infection among Brazilian hemophiliacs: a virological, clinical and epidemiological study

Human immunodeficiency virus type 1 and hepatitis C virus Co‐infection and viral subtypes at an HIV testing center in Brazil

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