Genotoxicity reduction in bagasse waste of sugar industry by earthworm technology

Abstract

The aim of the present study was to assess the genotoxicity reduction in post vermicompost feed mixtures of bagasse (B) waste using earthworm Eisenia fetida. The genotoxicity of bagasse waste was determined by using Allium cepa root chromosomal aberration assay. Bagasse was amended with cattle dung in different proportions [0:100 (B0) 25:75 (B25), 50:50 (B50), 75:25 (B75) and 100:0 (B100)] on dry weight basis. Genotoxic effects of initial and post vermicompost bagasse extracts were analysed on the root tips cells of Allium cepa. Root length and mitotic index (MI) was found to be increased in post vermicompost extracts when compared to initial bagasse waste. The maximum percent increase of root length was observed in the B50 bagasse extract (96.60 %) and the maximum MI was observed in B100 mixture (14.20 ± 0.60) 6 h treatment which was similar to the control. Genotoxicity analysis of post vermicompost extracts of bagasse revealed a 21–44 % decline in the aberration frequencies and the maximum reduction was found in B75 extract (44.50 %). The increase in root length and mitotic index, as well as decrease in chromosomal aberrations indicates that E. fetida has the ability to reduce the genotoxicity of the bagasse waste.

Toxicological effects of the waste of the sugarcane industry, used as agricultural fertilizer, on the test system Allium cepa

“…However, these compounds are not released by the process of solubilization of the sample, but have the ability to persist in the cell, acting later on them. These results are different from those obtained by Bhat et al (2014Bhat et al ( , 2016, which have found genotoxic effects for SCFC and SCB, prior to vermicompost practice. Based on our results and according to Siedlecka (1995), plants have a great potential to absorb metals, both essential for its metabolism (ex.…”

“…Probably, these associations still contained hydrosoluble contaminants with cytotoxic activity or inhibitors of cell division. Similar results were obtained by Bhat et al (2014), in which study the root growth and the MI of A. cepa was inhibited after exposure to pressmud sludge extract (pre and post-vermicompost with Eisenia fetida) for 3 and 6 h. Bhat et al (2016) also found a decreased MI in A. cepa cells induced by SCB, but the authors did not suggest a reason for this result because of most of cytotoxic effects were neutralized after vermicomposting. One of the possible causes of the decline of the MI may be related to the presence of Cu, Zn and Fe in the sample, as observed in the chemical analysis.…”

Pulping and Pretreatment Affect the Characteristics of Bagasse Inks for Three-dimensional Printing

“…The cytotoxicity effect is evidenced in the BS sample (Figure ), since it seems to have a higher load of silica particles, compared to the BHS pulp (Figure ). There are precedents about the elimination of cane bagasse toxicity by different methods. , In this work, the beneficial effect of the hydrothermal and alkaline treatments to reduce the cytotoxicity of bagasse CNF has been demonstrated. Importantly, these findings also emphasize the necessity of characterizing CNF properly, considering chemical, structural, and biological aspects.…”

Cytotoxic activity of ethanolic extracts of a selection of macromycetes

“…In addition, the model is sensitive to different types of chemical substances such as metals, pesticides, aromatic hydrocarbons, fabric industry residuals, disinfectants, ashes, environmental toxic contaminants (Leme and Marin-Morales 2009) or extracts of natural compound isolated from plants (Akinboro and Bakare 2007;Kundu and Ray 2017). Furthermore, this assay is acceptable for assessing cytotoxicity and genotoxicity (Bhat et al 2016;Ribeiro et al 2016).…”