Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures

Perreault, François; Melegari, Sílvia Pedroso; Costa, Cassiane da; Rossetto, Ana Letícia; Popovic, Radovan; Matias, William Gerson

doi:10.1016/j.scitotenv.2012.09.065

Cited by 116 publications

(71 citation statements)

References 52 publications

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“…In vitro studies indicate that CuONPs can induce DNA damage, DNA strand breaks and chromosome alterations in human lung epithelial, skin, peripheral blood and cancer cell lines [12][13][14][15][16][17]. In addition, DNA fragmentation, DNA methylation, and chromosome damage have also been reported in murine cell lines exposed to CuONPs [17,18]. Some in vivo studies reported an increase in the frequency of micronucleus formation in peripheral blood cells of mice treated acutely with CuONPs [19].…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Carmona

Inostroza‐Blancheteau

Obando

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Introductionmentioning

confidence: 99%

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Carmona

Inostroza‐Blancheteau

Obando

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Given the likely role of free-living protozoa in supporting growth of L. pneumophila reviewed in Lau and Ashbolt (30), it is also interesting that exposure to 80 g ml Ϫ1 CuO-NPs (30-nm diameter) induced significant changes in the composition of Tetrahymena thermophila membrane fatty acids (5). On a more cautionary note, cell lines exposed to 40 and 80 g ml Ϫ1 of CuO-NPs (20 to 40 nm) also resulted in cell damage or loss of viability and oxidative activity (10,31), indicating that these NPs may be unsuitable for the treatment of water that comes in direct contact with humans. While CuO-NPs as engineered nanomaterials showed potential for disinfection of legionellae, like all technological developments, unintended risks need to be fully examined, such as described in EPA's New Chemical Consent Orders and Significant New Use Rules (SNURs) (http://www.epa.gov/oppt /newchems/pubs/cnosnurs.htm).…”

Section: Discussionmentioning

confidence: 99%

Legionella pneumophila Transcriptional Response following Exposure to CuO Nanoparticles

Struewing

Buse

et al. 2013

Appl Environ Microbiol

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eCopper ions are an effective antimicrobial agent used to control Legionnaires' disease and Pontiac fever arising from institutional drinking water systems. Here, we present data on an alternative bactericidal agent, copper oxide nanoparticles (CuONPs), and its efficacy on Legionella pneumophila. In broth cultures, the CuO-NPs caused growth inhibition, which appeared to be concentration and exposure time dependent. The transcriptomic response of L. pneumophila to CuO-NP exposure was investigated by using a whole-genome microarray. The expression of genes involved in metabolism, transcription, translation, DNA replication and repair, and unknown/hypothetical proteins was significantly affected by exposure to CuO-NPs. In addition, expression of 21 virulence genes was also affected by exposure to CuO-NP and further evaluated by quantitative reverse transcription-PCR (qRT-PCR). Some virulence gene responses occurred immediately and transiently after addition of CuO-NPs to the cells and faded rapidly (icmV, icmW, lepA), while expression of other genes increased within 6 h (ceg29, legLC8, legP, lem19, lem24, lpg1689, and rtxA), 12 h (cegC1, dotA, enhC, htpX, icmE, pvcA, and sidF), and 24 h (legP, lem19, and ceg19), but for most of the genes tested, expression was reduced after 24 h of exposure. Genes like ceg29 and rtxA appeared to be the most responsive to CuO-NP exposures and along with other genes identified in this study may prove useful to monitor and manage the impact of drinking water disinfection on L. pneumophila.

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“…The toxicity of particles is often explained by oxidative damage mechanism (76). The ability of CuONPs to generate OTS in vitro studies has been demonstrated; however, information on the neurotoxicity of the CuONPs in vivo is low (77,78).…”

Section: Copper Oxide Nanoparticlesmentioning

confidence: 99%

Neurological Disorders and Oxidative Toxic Stress: A Role of Metal Nanoparticles

Mazdeh

Rahiminejad

Nili‐Ahmadabadi

et al. 2016

Jundishapur J Nat Pharm Prod

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Context: Oxidative stress is a hallmark of many types of neuropathology disorders and underlying mechanism in several neurodegenerative diseases and brain injuries. The CNS is particularly susceptible to oxidative toxic stress (OTS). Reactive oxygen spices are the basic inflammation and neurotoxicity mediators in ischemia/reperfusion injuries. The purpose of the present review is to provide an overview of some nanoparticles (NPs) in developing OTS conditions and neurological disorders. Evidence Acquisition: Here, a nanotechnology approach is evaluated using NPs in human neuronal protection against OTS. It may be wide therapeutic applications in the case of acute and/or chronic neurodegenerative disorders related to OTS. Results: In the brain of mice treated with nanosize TiO 2 , a significant association was found between the ability to induce the production of ROS and metabolic stress in intracellular environments and inflammatory responses in mice brai. The large surface area of AgNPs may efficiently facilitate the radicals generation including ROS in various organs. The production of ROS may cause DNA damage, cellular apoptosis, and activation of the mitogen activated protein kinase (MAPK) pathways which is responsible for regulating many cellular processes. Prolonged and excessive OTS may contribute to the activation of transcription factors and genes responsible for inflammation responses such as NF-κB and AP-1. Furthermore, OTS may contribute to the onset of neurodegenerative diseases. The ability of CuONPs to generate OTS in vitro studies has been demonstrated; however, information on the neurotoxicity of the CuONPs in vivo is low. Conclusions:The NPs-induced OTS may increase the pro-inflammatory responses. On the other hand, administration of antioxidants such as NAC and vitamin C and E prior to exposure to metal NPs significantly decreases OTS conditions.

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Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures

Cited by 116 publications

References 52 publications

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster

Legionella pneumophila Transcriptional Response following Exposure to CuO Nanoparticles

Neurological Disorders and Oxidative Toxic Stress: A Role of Metal Nanoparticles

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