Genotoxic biomonitoring study of population residing in pesticide contaminated regions in Göksu Delta: Micronucleus, chromosomal aberrations and sister chromatid exchanges

Ergene, Serap; Çelık, Ayla; Çavaş, Tolga; Kaya, Filiz

doi:10.1016/j.envint.2007.04.003

Cited by 65 publications

(30 citation statements)

References 46 publications

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“…Moreover, toxicants with oxidative stress potential might assault DNA leading to damage at molecular level (Jha, 2008;Hussain et al, 2014). Frequencies of nuclear and morphological anomalies in erythrocytes of fish such as irregular nucleus shape, poikilocytosis, binuclear, and micronuclei indicate genotoxic effects which could have taken place due to arsenic intoxication (Ergene et al, 2007;Strunjak-Perovic et al, 2010). A high proportion of abnormal shapes erythrocytes could also be due to increased erythropoiesis to compensate the impaired function and to eliminate the damaged cells (Witeska et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Ghaffar¹,

Hussain²,

Aslam³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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This study was conducted to assess hemato-biochemical and different protoplasmic changes, which are indicators of genotoxicity in erythrocytes of Labeo rohita exposed to arsenic and urea. For this purpose, 20 fish were kept in glass aquaria for acclimatization (5 days), randomly divided into five equal groups: A (control) and exposed to arsenic (in mg/L) + urea (in g/L) B (8+0.2), C (10+0.4), D (12+0.6) and E (15+0.8), respectively for 9 days. Blood samples with anticoagulant were collected at 3 rd , 6 th and 9 th experiment days for hematology. Serum was separated from blood samples collected without anticoagulants for biochemical studies. Results showed that exposure with arsenic and urea in higher concentrations (groups D and E) significantly decreased (P<0.05) erythrocyte counts, hemoglobin concentration, packed cell volume, mean corpuscular hemoglobin concentration, lymphocyte, and monocyte counts, accompanied by significant increase in mean corpuscular volume and leucocyte counts than those in control group (A). Alanine and aspartate amino transaminase activities at 6 th and 9 th days in high dose (groups D and E) were significantly increased whereas glucose and total protein decreased (P<0.05). It is concluded that arsenic has hemato-biochemical and genotoxic effects even at the low dose rate in the presence of nitrogenous chemicals in aquatic ecosystem.

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Section: Discussionmentioning

confidence: 99%

Untitled

Ghaffar¹,

Hussain²,

Aslam³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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“…Although there was no study with micronucleus assay in cyclohexanedione herbicides, MN test system was used by many investigators for other pesticide groups and they reported increases in MN formation (Zeljezic and Garaj-Vrhovac 2004;Yüzbasioglu et al 2006;Ergene et al 2007;Ali et al 2008;Revankar and Shyama 2009). These results support our findings.…”

Section: Discussionmentioning

confidence: 99%

“…Genotoxicity and cytotoxicity studies are frequently used to test numerous agrochemical compounds with different test systems (Ergene et al 2007;Lin and Garry 2000;Rakitsky et al 2000;Soloneski et al 2007Soloneski et al , 2008Soloneski and Larramendy 2010;Zeljezic et al 2006). These test systems are valuable and very well-known tools for the early and sensitive detection or estimation of genotoxic potential of chemicals.…”

Section: Introductionmentioning

confidence: 99%

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

et al. 2014

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This study evaluates the cytotoxic and genotoxic potential of alloxydim sodium using micronucleus (MN) assay, in human peripheral lymphocytes. MN assay was used to investigate the genotoxic effects of alloxydim sodium in human peripheral lymphocytes treated with 250, 500, 750, 1,000 lg/ml concentrations of alloxydim sodium for 24 and 48 h. Solvent, negative and positive controls were also used in the experiments in parallel. The obtained results were evaluated in statistical analyses by using Dunnett-t test (two sided) and p \ 0.05 was accepted as significant. Alloxydim sodium significantly increased the MN formation compared with the negative control, at both 750 and 1,000 lg/ml concentrations and treatment periods. We also evaluated the nuclear division index (NDI) for cytotoxicity of this pesticide in the experiment, and finally observed a significant decrease of the NDI values at all concentrations of alloxydim sodium and at both treatment periods.

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“…Both groups of authors did adjust statistical analysis for confounding factors. The incidence of chromosomal aberrations has been significantly affected (MANOVA, P<0.01) by pesticide exposure only in the study of Ergene et al, (2007) indicating that residential exposure to pesticides may also adversely affect genome integrity. Nevertheless, findings of studies documenting the presence of dicentric chromosomes and other complex chromosome-type aberrations in lymphocytes showed necessity of using novel cytogenetic approaches that may provide more detailed knowledge regarding the potential of pesticides to affect genome integrity and induce complex genome rearrangements considered as a driving force for carcinogenicity.…”

Section: Wwwintechopencommentioning

confidence: 99%

“…Costa et al (2006) detected complex structural alterations in lymphocytes of sprayers applying 33 different active ingredients without specifying the type. Ergene et al, (2007) detected dicentrics detected in residentially exposed subjects living in region contaminated mostly with organochlorines, organophosphates, carbamates, pyrethroids and benzoyl ureas. Both groups of authors did adjust statistical analysis for confounding factors.…”

Section: Wwwintechopencommentioning

confidence: 99%