Genosensors as an alternative diagnostic sensing approaches for specific detection of virus species: A review of common techniques and outcomes

Babaei, Abouzar; Pouremamali, Amir; Rafiee, Nastaran; Sohrabi, Hessamaddin; Mokhtarzadeh, Ahad; Guárdia, Miguel de la

doi:10.1016/j.trac.2022.116686

Cited by 21 publications

(13 citation statements)

References 275 publications

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“…The functioning of a genosensor is based on the immobilization of a probe (nucleic acid sequence: DNA, RNA or aptamers) onto the surface of a physical transducer, to sense its complementary sequence via hybridization [86] . Compared to other biosensors, genosensors are of great interest due to their ultra‐sensitivity towards the target sequence and applicability in early state detection [87] …”

Section: Voltammetric Sensors For Diagnosis Of Sars‐cov‐2 and Its Bio...mentioning

confidence: 99%

“…[86] Compared to other biosensors, genosensors are of great interest due to their ultra-sensitivity towards the target sequence and applicability in early state detection. [87] For the detection of SARS-CoV-2 nucleic acids, the sensing platform is modified with probe strands that are complementary to a target genome sequence. [55] Most of these strategies use a redox tags/labels to analyze the binding of the target since it can offer higher sensitivity to the sensors.…”

Section: Voltammetric Genosensors For Sars-cov-2mentioning

confidence: 99%

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Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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The recent events of outbreaks of corona virus caused by the Severe Acute Respiratory Syndrome Coronavirus −2(SARS‐CoV‐2) raised a concern and increased urge for quick and rapid detection techniques of disease causing pathogens. The detection of the virus with high sensitivity and accuracy holds great importance. Nowadays, the most commonly employed technique is the Quantitative Reverse Transcription Polymerase Chain Reaction test (qRT‐PCR) and antigen test. While RT‐PCR technique is time consuming, expensive and labour intensive, antigen tests though simple, can often give false positive and false negatives. In this context, electrochemical biosensors developed in recent times have been identified as a potential strategy to overcome the limitations of these common techniques. This review article summarizes the current advancements in the field of voltammetric sensors for the detection of COVID‐19 virus and various biomarkers associated with it.

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Section: Voltammetric Sensors For Diagnosis Of Sars‐cov‐2 and Its Bio...mentioning

confidence: 99%

Section: Voltammetric Genosensors For Sars-cov-2mentioning

confidence: 99%

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

2023

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“…The typical bioreceptors used for this purpose are oligonucleotides (mainly ssDNA) that are immobilized on the surface of modified electrodes [ 89 ]. DNA sensors are currently used in genetic diagnostics, food quality evaluation, or in the detection of a wide range of pathogens [ 90 , 91 ].…”

Section: Role Of Carbon Nanomaterials and Their Composites In The Des...mentioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

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To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

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“…Furthermore, since viral infections as well as virus-associated gastroenteritis are an important threat for humans around our planet, and especially in developing countries, there is an increasing requisition to detect these pathogens by advanced techniques [ 33 , 34 , 35 ]. Therefore, to overcome the restrictions of the existing diagnostic approaches [ 36 ], biosensors [ 37 , 38 ], as ultra-sensitive and specific minimized detection systems, were fabricated for early detection of virus-associated gastroenteritis from the stool and clinical specimens in recent years [ 26 , 39 , 40 ]. Consequently, their efforts resulted in designing several types of cheap, easy, fast, and highly sensitive biosensing platforms, such as immunoassay and nucleotide-assay-based sensors for the screening of viruses from clinical samples [ 36 , 39 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, to overcome the restrictions of the existing diagnostic approaches [ 36 ], biosensors [ 37 , 38 ], as ultra-sensitive and specific minimized detection systems, were fabricated for early detection of virus-associated gastroenteritis from the stool and clinical specimens in recent years [ 26 , 39 , 40 ]. Consequently, their efforts resulted in designing several types of cheap, easy, fast, and highly sensitive biosensing platforms, such as immunoassay and nucleotide-assay-based sensors for the screening of viruses from clinical samples [ 36 , 39 , 41 , 42 , 43 , 44 , 45 ]. Hence, in this review article, we focus on the newest improvements in the biosensor field and an overview of the various types of constructed and tested biosensing platforms for viral gastroenteritis detection.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

et al. 2022

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Gastroenteritis, as one of the main worldwide health challenges, especially in children, leads to 3–6 million deaths annually and causes nearly 20% of the total deaths of children aged ˂5 years, of which ~1.5 million gastroenteritis deaths occur in developing nations. Viruses are the main causative agent (~70%) of gastroenteritis episodes and their specific and early diagnosis via laboratory assays is very helpful for having successful antiviral therapy and reduction in infection burden. Regarding this importance, the present literature is the first review of updated improvements in the employing of different types of biosensors such as electrochemical, optical, and piezoelectric for sensitive, simple, cheap, rapid, and specific diagnosis of human gastroenteritis viruses. The Introduction section is a general discussion about the importance of viral gastroenteritis, types of viruses that cause gastroenteritis, and reasons for the combination of conventional diagnostic tests with biosensors for fast detection of viruses associated with gastroenteritis. Following the current laboratory detection tests for human gastroenteritis viruses and their limitations (with subsections: Electron Microscope (EM), Cell Culture, Immunoassay, and Molecular Techniques), structural features and significant aspects of various biosensing methods are discussed in the Biosensor section. In the next sections, basic information on viruses causing gastroenteritis and recent developments for fabrication and testing of different biosensors for each virus detection are covered, and the prospect of future developments in designing different biosensing platforms for gastroenteritis virus detection is discussed in the Conclusion and Future Directions section as well.

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Genosensors as an alternative diagnostic sensing approaches for specific detection of virus species: A review of common techniques and outcomes

Cited by 21 publications

References 275 publications

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

Voltammetric Sensors: A Versatile Tool in COVID‐19 Diagnosis and Prognosis

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Recent Advances in Early Diagnosis of Viruses Associated with Gastroenteritis by Biosensors

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