Genomics of Nitrogen Use Efficiency in Maize: From Basic Approaches to Agronomic Applications

Hirel, Bertrand; Lea, Peter J.

doi:10.1007/978-3-319-97427-9_16

Cited by 9 publications

(6 citation statements)

References 195 publications

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“…The majority of NH 4 + is subsequently used for glutamine formation by the glutamine synthase/glutamine oxoglutarate aminotransferase (GS/GOGAT) cycle in which reduced ferredoxin (Fd) or NADH is used as a reductant [9]. Consistent with the variety of metabolic roles and different specificities towards the reductants, NiR, GS, and GOGAT are present in maize in several isoforms; specifically, there are two types of NiR isoforms (Fd-NiR and NADH-NiR), two types of GOGAT isoforms (Fd-GOGAT and NADH-GOGAT), and two types of GS isoforms (cytosolic and chloroplastic GS-GS1 and GS2) [10,11]. In maize, the GS1 isoforms are encoded by five genes, and the GS2 isoforms are encoded by a single gene [12].…”

Section: Introductionmentioning

confidence: 99%

Exogenous DCPTA Increases the Tolerance of Maize Seedlings to PEG-Simulated Drought by Regulating Nitrogen Metabolism-Related Enzymes

Xie

et al. 2019

Agronomy

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2-(3,4-Dichlorophenoxy) triethylamine (DCPTA) regulates plant development; however, the molecular basis of this regulation is poorly understood. In this study, RNA sequencing (RNA-seq) analysis and physiological indexes of maize seedlings (three-leaf stage) treated with 15% polyethylene glycol (PEG) with/without DCPTA were investigated to explore the possible mechanism of exogenous DCPTA-improved drought tolerance. In the library pair comparisons of DCPTA vs. the control, PEG vs. the control, and PEG + DCPTA vs. PEG, totals of 19, 38 and 20 differentially expressed genes (DEGs) were classified as being involved in metabolic processes, respectively; totals of 5, 11, and 6 DEGs were enriched in the nitrogen (N) metabolic pathway, respectively. The genes encoding nicotinamide adenine dinucleotide-nitrate reductase (NADH-NR), ferredoxin-nitrite reductase (Fd-NiR), reduced ferredoxin-glutamate synthase (Fd-GOGAT), and chloroplastic glutamine synthetase (GS 2) were common in response to PEG-simulated drought stress with/without DCPTA treatment. Moreover, DCPTA maintained stable gene relative expression levels and protein abundances of NADH-NR, Fd-NiR, GS2, and Fd-GOGAT. Moreover, exogenous DCPTA partially mitigated PEG-simulated drought-induced reductions in the enzymatic activities of NR, nitrite reductase (NiR), glutamine synthase (GS), glutamine oxoglutarate aminotransferase (GOGAT), and transaminase, as well as in the contents of nitrate (NO 3 − ), nitrite (NO 2 − ) and soluble proteins and increases in the contents of ammonium (NH 4 + ) and free amino acids. Together, our results indicate that exogenous DCPTA improved plant growth and drought tolerance by regulating N-mechanism enzymatic activities involved in transcription and enzymatic protein synthesis.

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Section: Introductionmentioning

confidence: 99%

Exogenous DCPTA Increases the Tolerance of Maize Seedlings to PEG-Simulated Drought by Regulating Nitrogen Metabolism-Related Enzymes

Xie

et al. 2019

Agronomy

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“…Genes encoding GDH have been sequenced from several plant species: A. thaliana [139], maize [140] and triticale [141]. In wheat, the partial sequence of GDH demonstrated that GDH is less polymorphic than NADH-NAR1 and Fd-GOGAT between sequences obtained from diploid species T. monococcum, T. speltoides, T. tauschii and two cultivars of hexaploid T. aestivum, viz Arc Arche and Rec Récital [116].…”

Section: Glutamate Dehydrogenase (Gdh)mentioning

confidence: 99%

Genetic regulation of the traits contributing to wheat nitrogen use efficiency

et al. 2021

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“…Eight significant single nucleotide polymorphisms, SNPs, were found located in the coding or promoter regions of core senescence genes identified in a previous RNAseq experiment. One of the candidate genes, Zm00001d026501, codifies the plastidic glutamine synthetase 2 enzyme (GS2), which is involved in the assimilation of photorespiratory ammonium, as reported by several authors, and, in different species, these results work together with other genes [ 7 , 8 , 9 ].…”

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confidence: 91%