Genomic Signatures of Selection Associated With Litter Size Trait in Jining Gray Goat

Wang, Jun-Jie; Zhang, Teng; Chen, Qiu-Ming; Zhang, Ruiqian; Li, Lan; Cheng, Shun‐Feng; Shen, Wei; Lei, Chuzhao

doi:10.3389/fgene.2020.00286

Cited by 25 publications

(21 citation statements)

References 84 publications

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“…It is important to elucidate the genetic mechanisms of LS to efficiently utilize these prolific resources. Therefore, numerous studies have been separately performed to enhance our understanding of goat and sheep LS [3][4][5][6][7]. Compared with sheep, knowledge of hircine prolificacy is in its infancy.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analyses Reveal Genetic Convergence of Prolificacy between Goats and Sheep

Lin

Jiang

et al. 2021

Genes

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The litter size of domestic goats and sheep is an economically important trait that shows variation within breeds. Strenuous efforts have been made to understand the genetic mechanisms underlying prolificacy in goats and sheep. However, there has been a paucity of research on the genetic convergence of prolificacy between goats and sheep, which likely arose because of similar natural and artificial selection forces. Here, we performed comparative genomic and transcriptomic analyses to identify the genetic convergence of prolificacy between goats and sheep. By combining genomic and transcriptomic data for the first time, we identified this genetic convergence in (1) positively selected genes (CHST11 and SDCCAG8), (2) differentially expressed genes (SERPINA14, RSAD2, and PPIG at follicular phase, and IGF1, GPRIN3, LIPG, SLC7A11, and CHST15 at luteal phase), and (3) biological pathways (genomic level: osteoclast differentiation, ErbB signaling pathway, and relaxin signaling pathway; transcriptomic level: the regulation of viral genome replication at follicular phase, and protein kinase B signaling and antigen processing and presentation at luteal phase). These results indicated the potential physiological convergence and enhanced our understanding of the overlapping genetic makeup underlying litter size in goats and sheep.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Analyses Reveal Genetic Convergence of Prolificacy between Goats and Sheep

Lin

Jiang

et al. 2021

Genes

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“…The Macheng black goat is highly fertile, the kidding rate and fecundity rate are approximately 219% and 346% ( Tao et al, 2018 ). Recent studies indicate that a high ovulation rate can increase the probability of higher litter size ( Wang et al, 2020 ). As a high propagation capability goat breed, how lncRNAs regulate its follicular development is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis

et al. 2022

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Long noncoding RNAs (lncRNAs) were identified recently as a large class of noncoding RNAs (ncRNAs) with a length ≥200 base pairs (bp). The function and mechanism of lncRNAs have been reported in a growing number of species and tissues. In contrast, the regulatory mechanism of lncRNAs in the goat reproductive system has rarely been reported. In the present study, we sequenced and analyzed the lncRNAs using bioinformatics to identify their expression profiles. As a result, 895 lncRNAs were predicted in the pre-ovulatory ovarian follicles of goats. Eighty-eight lncRNAs were differentially expressed in the Macheng black goat when compared with Boer goat. In addition, the lncRNA XR_311113.2 acted as a sponge of chi-miR-424-5p, as assessed via a luciferase activity assay. Taken together, our findings demonstrate that lncRNAs have potential effects in the ovarian follicles of goats and may represent a promising new research field to understand follicular development.

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“…In the current study, we analyzed the polymorphic variations of the gene coding for the GDF9, a member of the transforming growth factor β superfamily. This superfamily has a vital role in female fertility and the GDF9 protein is essential for ovarian follicular development in sheep, especially during the early stages of folliculogenesis [35]. This study focused on scanning for the G1 point mutation located in exon 1 of the GDF9 gene in three Sudanese Desert sheep tribes.…”

Section: Source Of Variation Df Sm Ms F-ratio Pr>fmentioning

confidence: 99%

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

et al. 2021

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Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

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Genomic Signatures of Selection Associated With Litter Size Trait in Jining Gray Goat

Cited by 25 publications

References 84 publications

Genome-Wide Analyses Reveal Genetic Convergence of Prolificacy between Goats and Sheep

Genome-Wide Analyses Reveal Genetic Convergence of Prolificacy between Goats and Sheep

Characterization of XR_311113.2 as a MicroRNA Sponge for Pre-ovulatory Ovarian Follicles of Goats via Long Noncoding RNA Profile and Bioinformatics Analysis

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

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