Genomic comparison between two Inonotus hispidus strains isolated from growing in different tree species

Abstract

Inonotus hispidus mainly growing in broad-leaved trees, including Morus alba, Fraxinus mandshurica, and Ulmus macrocarpa etc. The fruiting body of I. hispidus growing in M. alba (hereafter as MA) is used as a traditional Chinese medicine “Sanghuang”. However, differences between the genetic material basis of I. hispidus growing in other tree species have not been reported. Therefore, in this paper, the genomic comparison between MA and I. hispidus growing in F. mandshurica (hereafter as FM) were studied. The whole genome of MA monokaryon was sequenced by Illumina combined with Pac Bio platform. Next, genome assembly, genome component prediction and genome functional annotation were performed. Comparative genomics analysis was performed between FM monokaryon and MA monokaryon, using MA as the reference. The results showed that, MA had 24 contigs with a N50 length of 2.6 Mb. Specifically, 5,342, 6,564, 1,595, 383 and 123 genes were annotated from GO, KEGG, KOG, CAZymes and CYP450, respectively. Moreover, comparative genomics showed that, the coding genes and total number of genes annotated in different databases of FM were higher than that of MA. This study provides a foundation for the medicinal application of FM as MA from the perspective of genetic composition.

Whole genome sequencing and analysis of Inonotus hispidus isolated from the Chengde Mountain Resort, China

“…This result is consistent with previous reports, in which secondary metabolic gene clusters involved in terpene regulation were most frequently identi ed in the genomes of the Xiajin strain (Shandong Province) and the Aksu Prefecture strain (Xinjiang Province) of I. hispidus. Speci cally, 8 of 15 secondary metabolism gene clusters were found to be associated with terpene regulation in the Xiajin strain and 12 of 20 in the Aksu Prefecture strain (Zhang, Feng et al 2022, Wang, Bao et al 2023). Hence, triterpenes serve as signi cant secondary metabolites in I. hispidus and play important roles in biological functions that promote human health.…”

“…The I. hispidus strain from Chengde used in this study was isolated from a mulberry tree over 300 years old at the Chengde Mountain Resort, giving this strain signi cant historical and research value. Although the I. hispidus strains of Aksu (Xinjiang), Harbin (Heilongjiang), Xiajin (Shandong), and Linqing (Shandong) have been sequenced, whole genome sequencing of the I. hispidus strain from Chengde Mountain Resort still has great signi cance for studying the genomic and functional differences of I. hispidus from different regions, and to enrich the genomic diversity of I. hispidus (Tang, Jin et al 2022, Zhang, Feng et al 2022, Jinglin 2023, Wang, Bao et al 2023). In addition, access to genomic information on I. hispidus "CDMR" strain can contribute to genetic and genomic research on its nutritional and therapeutic traits.…”