Genomic characterization of fourEscherichia colistrains isolated from oral lichen planus biopsies

Abstract

Oral lichen planus (OLP) is a chronic T cell-mediated inflammatory disease that affects the mucus membrane of the oral cavity. We previously proposed a potential role of intracellular bacteria detected within OLP lesions in the pathogenesis of OLP and isolated four Escherichia coli strains from OLP tissues that were phylogenetically close to K-12 MG1655 strain. We sequenced the genomes of the four OLP-isolated E. coli strains and generated 6.71 Gbp of Illumina MiSeq data (166-195x coverage per strain). The size of the assembled draft genomes was 4.69 Mbp, with a GC content of 50.7%, in which 4360 to 4367 protein-coding sequences per strain were annotated. We also identified 368 virulence factors and 53 antibiotic resistance genes. Comparative genomics revealed that the OLP-isolated strains shared more pangenome orthologous groups with pathogenic strains than did the K-12 MG1655 strain, a derivative of K-12 strain isolated from human feces. Although the OLP-isolated strains did not have the major virulence factors (VFs) of the pathogenic strains, a number of VFs involved in adherence/invasion, colonization, or systemic infection were identified. The genomic characteristics of E. coli first isolated from the oral cavity would benefit future investigations on the pathogenic potential of these bacteria.

Advancements in the Relationship Between Oral Lichen Planus and Microbial Infections

“…The researchers also discovered that when the relative abundance of most flora in OLP tissue declined, the relative abundance of the E. coli K12 strain grew dramatically. Furthermore, researchers [17] discovered 368 different types of virulence factors (VFS) from OLP patients' E. coli strains. It was discovered by detection that a variety of VFS can assist in bacteria invasion and colonization.…”

Molecular detection of pathogenic bacteria (K. pneumoniae, P. aeruginosa, and E.coli) from human saliva

“…P. aeruginosa is a Gram-negative rod-shaped microorganism that is pathogenic to humans, animals, and plants (Diggle & Whiteley 2020). E. coli is anaerobic, single straight rodshaped bacteria, can be motile or nonmotile and have the ability of both a respiratory metabolism and fermentation and do not produce the oxidase enzyme (Min et al 2021).…”