Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan

Abstract

Background Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA’s virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnostic assays. The objective of the present study is to perform whole-genome sequencing of MRSA isolated from Sudan using Illumina Next Generation Sequencing (NGS) platform. Results The genome of MRSA strain SO-1977 consists of 2,827,644 bp with 32.8% G + C, 59 RNAs and 2629 predicted coding sequences (CDSs). The genome has 26 systems, one of which is the major class in the disease virulence and defence. A total of 83 genes were annotated to virulence disease and defence category some of these genes coding as functional proteins. Based on genome analysis, it is speculated that the SO-1977 strain has resistant genes to Teicoplanin, Fluoroquinolones, Quinolone, Cephamycins, Tetracycline, Acriflavin and Carbapenems. The results revealed that the SO-1977, strain isolated from Sudan has a wide range of antibiotic resistance compared to related strains. Conclusion The study reports for the first time the whole genome sequence of Sudan MRSA isolates. The release of the genome sequence of the strain SO-1977 will avail MRSA in public databases for further investigations on the evolution of resistant mechanism and dissemination of the -resistant genes of MRSA.

First insights into Molecular basis Identification of 16s Ribosomal RNA Gene of Staphylococcus aureus Isolated from Sudan

“…The isolates distributed in the three branches with those from Japan (LC508802), Nigeria (MN606199), Germany (MF664194), Italy (MN811085), Ethiopia (MK217496), Iraq (MN555444), Pakistan (MN611106), Bangladesh (MN611246), Egypt (MN556575), China (MN652637), India (MK165143) and USA (MF385261). The current results agree with previouly reported study in Sudan by Mohamed et al that found genetic similarity in genomic sequence analyses of different S.aureus strains in the world in relation with isolated strains [26]. Similar to the current study results, Raed et al indicated that genetic dimension between Iraq and the isolates of the world is extremely relative, and 16s rRNA analysis is considered a good discrimination approach for distinguishing unrelated isolates [27].…”

First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan

“…The isolates distributed in the three branches with those from Japan (LC508802), Nigeria (MN606199), Germany (MF664194), Italy (MN811085), Ethiopia (MK217496), Iraq (MN555444), Pakistan (MN611106), Bangladesh (MN611246), Egypt (MN556575), China (MN652637), India (MK165143) and USA (MF385261). The current results agree with previously reported study in Sudan by Mohamed et al [ 28 ] that found genetic similarity in genomic sequence analyses of different S. aureus strains in the world in relation with isolated strains. Similar to the current study results, Raed et al .…”

Characterization of methicillin-resistant Staphylococcus aureus through genomics approach

“…Many of these established techniques are costly and time-consuming, and the discriminatory abilities of these techniques are also different (Du et al 2011 ). However, the spa typing method has been considered as a rapid and inexpensive method for genotyping and it provides high discriminating power than other typing methods (Shittu et al 2011 ; O’Hara et al 2016 ; Goudarzi et al 2017 ; Ali et al 2019 ; Rezai et al 2019 ; Kareem et al 2020 ). Both phenotypic and molecular typing methods have been used widely to detect and differentiate several MRSA strains, but these techniques have certain limitations in infection control and investigating the nosocomial transmission as these techniques provide low resolution and more time-consuming.…”