Genomic analyses of aminergic signaling systems (dopamine, octopamine and serotonin) in Daphnia pulex

Low environmental levels of neuro-active pharmaceuticals alter phototactic behaviour and reproduction in Daphnia magna

“…It was also shown that SSRIs de-regulate genes related to serotonin signalling pathways in D. magna (Campos et al, 2013a). Putative 5-HT 1,7 receptors and serotonin transporter (SERT) gene homologues have also been found in the D. magna close relative D. pulex genome (McCoole et al, 2012a). The above mentioned data, however, do not demonstrate that SSRI act similarly than humans.…”

Trace Amines and Their Receptors

“…Dedicated tyrosine decarboxylase enzymes are found in various invertebrate species (Livingstone and Tempel, 1983;Ishida and Ozaki, 2012;McCoole et al, 2012;Christie et al, 2014) and, at least in Drosophila, distinct neuronal and non-neuronal isozymes exist (Cole et al, 2005). Although an AADC-like, DOPA decarboxylase enzyme may also be present, this does not appear to act on L-tyrosine (Han et al, 2010).…”

In silico characterization of the insect diapause-associated protein couch potato (CPO) in Calanus finmarchicus (Crustacea: Copepoda)

“…Genome mining was accomplished as described in several earlier publications (Christie et al, 2011a;McCoole et al, 2011;Tilden et al, 2011;McCoole et al, 2012aMcCoole et al, , 2012b using BLAST+ 2.2.23 software (downloaded from the National Center for Biotechnology Information, Bethesda, MD, USA; ftp://ftp.ncbi.nlm.nih.gov/blast/executables/ blast+/) and the Beta3-release of the D. pulex Genes2010 frozen genome assembly (Indiana University Genome Informatics Laboratory and Center for Genomics and Bioinformatics at Indiana University, Bloomington, IN, USA; http://wfleabase.org/; Colbourne et al, 2011); the sequence of first of the two identified C. finmarchicus CPO isoforms was used as the protein query (see 3.1.2).…”

“…Moreover, on-line software programs available to search these data sets, translate nucleotide sequences, and predict the post-translational processing and structural domains present in the deduced proteins, make genome/transcriptome mining a rapid and readily accessible approach for protein discovery in crustaceans (e.g. Christie et al, 2008;Gard et al, 2009;Ma et al, 2009Ma et al, , 2010Christie et al, , 2011aMcCoole et al, 2011;Tilden et al, 2011;McCoole et al, 2012aMcCoole et al, , 2012b. Here, we have used both publicly-accessible expressed sequence tags (ESTs) and our own 454 pyrosequencing data to search for homologs of CPO in C. finmarchicus and other crustaceans.…”