GENOMES OF THE AUSTRALIAN WILD SPECIES OF COTTON. I.GOSSYPIUM STURTIANUM, THE STANDARD FOR THE C GENOME

Abstract

A detailed karyotype of Gossypium sturtianum Willis is presented to establish the C genome karyotype for comparison with other Australian wild species of cotton. Gossypium sturtianum has one chromosome with a median centromere, eight with slightly submedian centromeres, two with submedian centromeres and two with highly submedian centromeres. Chromosomes 8 and 9 have satellites. The total complement length is 75.04 micrometers.

Karyotype analysis of gossypium bickii prokh.

“…Its karyotype data was correspondently listed in Table 1 and Fig. 2 with the data reported by Edwards (1979) according to the parameters of chromosome morphology. These two data were contrasted and analysed as follows.…”

“…Furthermore, the positions of some m or sm chromosome in both data were also different. wards 1977, 1979, Edwards and Mirza 1979, Nie Ruzhi and Li Maoxue 1985. The result of this test also proved that the chromosome of G. bickii was smaller than that of C and A genomes, but larger than that of D genome.…”

“…In brief, on the basis of karyotype analysis of G. bickii, combined with the result of its phenetic analysis (Fryxell 1971), DNA content of cells (Edwards et a!. 1975) and electrophero gram of seed protein (Johnson and Thein 1970), classifying G. bickii as a new G genome in Gossypium had a lot of scientific foundation.…”

Genomes of the Australian Wild Species of Cotton. III.Gossypium Australe

“…The primary radicle as collected, placed in a saturated solution of mono-bromonaphthalene for 2.5 hours, and fixed in 3:1 ethanol-acetic acid for two days to two weeks. The root-tips were stained in Schiff's reagent and metaphase plates were drawn using the procedure of EDWARDS ( 1977 ). All measurements presented in Table 1 are the means of five metaphase plates.…”

The karyotype of Hampea rovirosae standl.

“…The root tips were stained with Feulgen's stain and the chromosomes were drawn and measured using the procedures outlined by Edwards (1977). Ten cells were selected in which all 26 chromosomes could be measured, and all means of Table 1 were determined from these cells.…”